Microinjection of Cre recombinase protein into zygotes enables specific deletion of two eukaryotic selection cassettes and enhances the expression of a DsRed2 reporter gene in Ccr2/Ccr5 double-deficient mice

Genesis

Volumn 47, Issue 8, 2009, Pages 545-558

  Luckow, Bruno ^a   Hänggli, Amy ^b   Maier, Holger ^a,c   Chilla, Silvia ^a   Loewe, Robert P ^a   Dehmel, Stefan ^a   Schlöndorff, Detlef ^a,d   Loetscher, Pius ^b   Zerwes, Hans Günter ^b   Müller, Matthias ^b  

^a LUDWIG MAXIMILIANS UNIVERSITY   (Germany)

^b NOVARTIS PHARMA AG   (Switzerland)

^c INSTITUTE OF EPIDEMIOLOGY   (Germany)

^d MOUNT SINAI SCHOOL OF MEDICINE   (United States)

Author keywords

Bacteriophage P1 recombinase Cre; Chemokine receptors; Knockout mice; Microinjections

Indexed keywords

CHEMOKINE RECEPTOR CCR2; CHEMOKINE RECEPTOR CCR5; CRE RECOMBINASE; ENHANCED GREEN FLUORESCENT PROTEIN; GENE PRODUCT; HYBRID PROTEIN; PROTEIN DSRED2; UNCLASSIFIED DRUG;

ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; BLASTOCYST; CONTROLLED STUDY; EMBRYO; EMBRYONIC STEM CELL; EUKARYOTE; FEMALE; GENE CASSETTE; GENE DELETION; GENE EXPRESSION; GENE INACTIVATION; GENE SEQUENCE; GENETIC RECOMBINATION; GENETIC TRANSFECTION; MOUSE; MOUSE STRAIN; NONHUMAN; PRIORITY JOURNAL; REPORTER GENE; ZYGOTE;

ANIMALS; BASE SEQUENCE; DNA PRIMERS; EMBRYONIC STEM CELLS; GENE DELETION; GENES, REPORTER; GENETIC VECTORS; GERM CELLS; IN SITU HYBRIDIZATION, FLUORESCENCE; INTEGRASES; MICE; MICE, INBRED BALB C; MICE, INBRED C57BL; MICROINJECTIONS; RECEPTORS, CCR2; RECEPTORS, CCR5; RECOMBINATION, GENETIC; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; ZYGOTE;

EUKARYOTA; MUS;

EID: 70349229789     PISSN: 1526954X     EISSN: 1526968X     Source Type: Journal    
DOI: 10.1002/dvg.20531     Document Type: Article

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