Identification of vhhP2, a novel genetic marker of Vibrio harveyi, and its application in the quick detection of V. harveyi from animal specimens and environmental samples

Journal of Applied Microbiology

Volumn 107, Issue 4, 2009, Pages 1251-1257

  Sun, K ^a,b   Hu, Y H ^a,b   Zhang, X H ^c   Bai, F F ^c   Sun, L ^a  

^a INSTITUTE OF OCEANOLOGY   (China)

^b UNIVERSITY OF CHINESE ACADEMY OF SCIENCES   (China)

^c OCEAN UNIVERSITY OF CHINA   (China)

Author keywords

Genetic marker; PCR detection; Phylogenetic; Vibrio harveyi

Indexed keywords

ANIMALS; BIOLOGICAL MEMBRANES; GENE ENCODING; POLYMERASE CHAIN REACTION;

CRUDE SAMPLES; ENVIRONMENTAL SAMPLE; GENETIC MARKERS; ITS APPLICATIONS; PCR DETECTION; PHYLOGENETICS; QUICKEST DETECTION; SPECIES SPECIFICS; SPECIFIC DETECTION; VIBRIO HARVEYI;

PHYLOGENETIC;

BACTERIAL PROTEIN; OUTER MEMBRANE PROTEIN; UNCLASSIFIED DRUG; VIBRIO HARVEYI HYPOTHETICAL PROTEIN 2;

BACTERIUM; DETECTION METHOD; ENVIRONMENTAL ASSESSMENT; GENETIC MARKER; IDENTIFICATION METHOD; PATHOGEN; PHYLOGENETICS; POLYMERASE CHAIN REACTION; PROTEIN; RELATEDNESS;

ANIMAL TISSUE; ARTICLE; BACTERIAL STRAIN; BACTERIUM DETECTION; BACTERIUM IDENTIFICATION; BACTERIUM ISOLATE; GENE IDENTIFICATION; GENETIC CODE; GENETIC CONSERVATION; GENETIC MARKER; GEOGRAPHIC DISTRIBUTION; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PROTEIN ANALYSIS; PROTEIN FUNCTION; SPECIES DIFFERENCE; VIBRIO ALGINOLYTICUS; VIBRIO HARVEYI; VIBRIO PARAHAEMOLYTICUS;

ANIMALIA; VIBRIO HARVEYI;

EID: 70349099962     PISSN: 13645072     EISSN: 13652672     Source Type: Journal    
DOI: 10.1111/j.1365-2672.2009.04304.x     Document Type: Article

References (38)

1. Austin, B. (1988) Marine Microbiology. Cambridge, UK : Cambridge University Press.
2. Austin, B. Zhang, X.H. (2006) Vibrio harveyi: a significant pathogen of marine vertebrates and invertebrates. Lett Appl Microbiol 43, 119 124.
3. Chen, C.Y., Wu, K.M., Chang, Y.C., Chang, C.H., Tsai, H.C., Liao, T.L., Liu, Y.M., Chen, H.J. et al. (2003) Comparative genome analysis of Vibrio vulnificus, a marine pathogen. Genome Res 13, 2577 2587.
4. Conejero, M.J.U. Hedreyda, C.T. (2003) Isolation of partial toxR gene of Vibrio harveyi and design of toxR-targeted PCR primers for species detection. J Appl Microbiol 95, 602 611.
5. Conejero, M.J.U. Hedreyda, C.T. (2004) PCR detection of hemolysin (vhh) gene in Vibrio harveyi. J Gen Appl Microbiol 50, 137 142.
6. Dorsch, M., Lane, D. Stackebrandt, E. (1992) Towards a phylogeny of the genus Vibrio based on 16S rRNA sequences. Int J Syst Bacteriol 42, 58 63.
7. Gomez-Gil, B., Thompson, F.L., Thompson, C.C. Swings, J. (2003) Vibrio rotiferianus sp. nov., isolated from cultures of the rotifer Brachionus plicatilis. Int J Syst Evol Microbiol 53, 239 243.
8. Gomez-Gil, B., Soto-Rodríguez, S., García-Gasca, A, Roque, A., Vazquez-Juarez, R., Thompson, F.L. Swings, J. (2004) Molecular identification of Vibrio harveyi-related isolates associated with diseased aquatic organisms. Microbiology 150, 1769 1777.
9. Jayasree, L., Janakiram, P. Madhavi, R. (2006) Characterization of Vibrio spp. associated with diseased shrimp from culture ponds of Andhra Pradesh (India). J World Aquac Soc 37, 523 532.
10. Lavilla-Pitogo, C.R., Baticados, M.C.L., Cruz-Lacierda, E.R. de la Pena, L.D. (1990) Occurrence of luminous bacterial disease of Penaeus monodon larvae in the Philippines. Aquaculture 91, 1 13.
11. Lee, S.E., Shin, S.H., Kim, S.Y., Kim, Y.R., Shin, D.H., Chung, S.S., Lee, Z.H., Lee, J.Y. et al. (2000) Vibrio vulnificus has the transmembrane transcription activator ToxRS stimulating the expression of the hemolysin gene vvhA. J Bacteriol 182, 3405 3415.
12. Lightner, D.V. (1993) Diseases of cultured penaeid shrimp. In CRC Handbook of Mariculture, Crustacean Aquaculture ed. Mcvey, J.P. pp. 393 486. Boca Raton, FL : CRC Press.
13. Lin, Z., Kumagai, K., Baba, K., Mekalanos, J.J. Nishibuchi, M. (1993) Vibrio parahaemolyticus has a homolog of the Vibrio cholerae toxRS operon that mediates environmentally induced regulation of the thermostable direct hemolysin gene. J Bacteriol 175, 3844 3855.
14. Liu, P.C., Lee, K.K., Yii, K.C., Kou, G.H. Chen, S.N. (1996) Isolation of Vibrio harveyi from diseased kuruma prawns Penaeus japonicus. Curr Microbiol 33, 129 132.
15. Nagpal, M.L., Fox, K.F. Fox, A. (1998) Utility of 16S-23S rRNA spacer region methodology: how similar are interspace regions within a genome and between strains for closely related organisms? J Microbiol Methods 33, 211 219.
16. Oakey, H.J., Levy, N., Bourne, D.G., Cullen, B. Thomas, A. (2003) The use of PCR to aid in the rapid identification of Vibrio harveyi isolates. J Appl Microbiol 95, 1293 1303.
17. Osoril, C.R. Klose, K.E. (2000) A region of the transmembrane regulatory protein ToxR that tethers the transcriptional activation domain to the cytoplasmic membrane displays wide divergence among Vibrio species. J Bacteriol 182, 526 528.
18. Owens, L. Busico-Salcedo, N. (2006) Vibrio harveyi: pretty problems in paradise. In The Biology of Vibrios ed Thompson, F.L., Austin, B. Swings, J. pp. 266 280 Washington DC : ASM Press.
19. Pang, L., Zhang, X.H., Zhong, Y., Chen, J., Li, Y. Austin, B. (2006) Identification of Vibrio harveyi using PCR amplification of the toxR gene. Lett Appl Microbiol 43, 249 255.
20. Pass, D.A., Dybadahl, R. Manion, M.M. (1987) Investigations into the causes of mortality in the pearl oyster, Pinctata maxima (Jamson), in western Australia. Aquaculture 65, 149 169.
21. Prayitno, S.B. Latchford, J.W. (1995) Experimental infections of crustaeans with luminous bacteria related to Photobacterium and Vibrio. Effect of salinity and pH on infectiosity. Aquaculture 132, 105 112.
22. Robertson, P.A.W., Xu, H.S. Austin, B. (1998) An enzyme-linked immunosorbent assay (ELISA) for the detection of Vibrio harveyi in Penaeid shrimp and water. J Microbiol Methods 34, 31 39.
23. Ruangpan, L. Kitao, T. (1991) Vibrio bacteria isolated from black tiger shrimp, Penaeus monodon Fabricuius. J Fish Dis 14, 385.
24. Sambrook, J., Fritsch, E.F. Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, 2nd edn. Cold Spring Harbor, NY : Cold Spring Harbor Laboratory.
25. San Luis, B.B. Hedreyda, C.T. (2006) Analysis of a gene (vch) encoding hemolysin isolated and sequenced from Vibrio campbellii. J Gen Appl Microbiol 52, 303 313.
26. Sawabe, T., Kita-Tsukamoto, K. Thompson, F.L. (2007) Inferring the evolutionary history of vibrios by means of multilocus sequence analysis. J Bacteriol 189, 7932 7936.
27. Sithigorngul, P., Rukpratanporn, S., Pecharaburanin, N., Suksawat, P., Longyant, S., Chaivisuthangkura, P. Sithigorngul, W. (2007) A simple and rapid immunochromatographic test strip for detection of pathogenic isolates of Vibrio harveyi. J Microbiol Methods 71, 256 264.
28. Sparagano, O.A.E., Robertson, P.A.W., Purdom, I., McInnes, J., Li, Y., Yu, D.H., Du, Z.J., Xu, H.S. et al. (2002) PCR and molecular detection for differentiating Vibrio species. Ann N Y Acad Sci 969, 60 65.
29. Tendencia, E.A. (2002) Vibrio harveyi isolated from cage-culture seabass Lates calcarifer Bloch in the Philippines. Aquac Res 33, 455 458.
30. Thaithongnum, S., Ratanama, P., Weeradechapol, K., Sukhoom, A. Vuddhakul, V. (2006) Detection of V. harveyi in shrimp postlarvae and hatchery tank water by the Most Probable Number technique with PCR. Aquaculture 261, 1 9.
31. Thompson, F.L., Iida, T. Swings, J. (2004) Biodiversity of vibrios. Microbiol Mol Biol Rev 68, 403 431.
32. Thompson, F.L., Gomez-Gil, B., Teresa, A., Vasconcelos, R. Sawabe, T. (2007) Multilocus sequence analysis reveals that Vibrio harveyi and V. campbellii are distinct species. Appl Environ Microbiol 73, 4279 4285.
33. Vandenberghe, J., Thompson, F., Gomez-Gil, B. Swings, J. (2003) Phenotypic diversity amongst isolates from marine aquaculture systems. Aquaculture 219, 9 20.
34. Yu, C.S., Chen, Y.C., Liu, C.H. Hwang, J.K. (2006) Prediction of protein subcellular localization. Proteins 64, 643 651.
35. Zhang, W. Sun, L. (2007) Cloning, characterization and molecular application of a beta-agarase gene from Vibrio sp. strain V134. Appl Environ Microbiol 73, 2825 2831.
36. Zhang, X.H., Meaden, P.G. Austin, B. (2001) Duplication of hemolysin genes in a virulent isolate of Vibrio harveyi. Appl Environ Microbiol 67, 3161 3167.
37. Zhang, M., Sun, K. Sun, L. (2008a) Regulation of autoinducer 2 production and luxS expression in a pathogenic Edwardsiella tarda strain. Microbiology 154, 2060 2069.
38. Vh, a serine protease and a protective immunogen from a pathogenic Vibrio harveyi strain. Appl Environ Microbiol 74, 6254 6262.