Application of the SILAC (stable isotope labelling with amino acids in cell culture) technique in quantitative comparisons for tissue proteome expression

Biotechnology and Applied Biochemistry

Volumn 54, Issue 1, 2009, Pages 11-20

  Xu, Yuhuan ^a   Liang, Shufang ^a   Shen, Guobo ^a   Xu, Xuejiao ^b   Liu, Qingping ^b   Xu, Zhizhong ^a   Gong, Fengming ^a   Tang, Minghai ^a   Wei, Yuquan ^a  

^a SICHUAN UNIVERSITY   (China)

^b FUDAN UNIVERSITY   (China)

Author keywords

2H leucine labelling; Cytochrome c oxidase; Quantitative proteomics; Stable isotope labelling with amino acids in cell culture(SILAC); Tissue proteome

Indexed keywords

[2H]LEUCINE LABELLING; CANCEROUS TISSUES; CULTURED CELL; CYTOCHROME C OXIDASE; EXPRESSION LEVELS; HYDRATASE; IMMUNOHISTOCHEMISTRY; IN-CELL; INTERNAL STANDARDS; ISOFORMS; KIDNEY TISSUES; MASS SPECTRA; PAGE ANALYSIS; PEAK INTENSITY; PEPTIDE FRAGMENTS; PROTEIN EXPRESSION LEVELS; PROTEOME ANALYSIS; PROTEOMES; QUANTITATIVE COMPARISON; QUANTITATIVE PROTEOMICS; QUANTITATIVE TECHNIQUES; RENAL CARCINOMA; STABLE ISOTOPE LABELLING WITH AMINO ACIDS IN CELL CULTURE(SILAC); STABLE ISOTOPES; TISSUE PROTEINS; TUMOURS; VIMENTIN; WESTERN-BLOT ANALYSIS;

AMINATION; AMINES; AMINO ACIDS; ANIMAL CELL CULTURE; CELL MEMBRANES; FARM BUILDINGS; HISTOLOGY; ISOTOPES; MASS SPECTROMETERS; MASS SPECTROMETRY; MOLECULAR BIOLOGY; ORGANIC ACIDS; PROTEINS; TUMORS;

CELL CULTURE;

CYTOCHROME C OXIDASE; CYTOCHROME C OXIDASE SUBUNIT 4 ISOFORM 1; ENOYL COENZYME A HYDRATASE; LEUCINE; PEPTIDE FRAGMENT; UNCLASSIFIED DRUG; VIMENTIN; DEUTERIUM; PROTEOME;

ARTICLE; CELL CULTURE; CONTROLLED STUDY; DOWN REGULATION; HUMAN; HUMAN CELL; HUMAN TISSUE; IMMUNOHISTOCHEMISTRY; ISOTOPE LABELING; KIDNEY PARENCHYMA; MASS SPECTROMETRY; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN BLOOD LEVEL; PROTEIN EXPRESSION; PROTEOMICS; QUANTITATIVE ANALYSIS; STABLE ISOTOPE LABELLING WITH AMINO ACID IN CELL CULTURE; UPREGULATION; WESTERN BLOTTING; AMINO ACID SEQUENCE; BIOSYNTHESIS; CELL LINE; CHEMISTRY; KIDNEY TUMOR; METABOLISM; METHODOLOGY; MOLECULAR GENETICS; REPRODUCIBILITY; TANDEM MASS SPECTROMETRY; TUMOR CELL CULTURE;

AMINO ACID SEQUENCE; CELL LINE; DEUTERIUM; DOWN-REGULATION; ELECTRON TRANSPORT COMPLEX IV; ENOYL-COA HYDRATASE; HUMANS; IMMUNOHISTOCHEMISTRY; ISOTOPE LABELING; KIDNEY NEOPLASMS; LEUCINE; MOLECULAR SEQUENCE DATA; PROTEOME; PROTEOMICS; REPRODUCIBILITY OF RESULTS; TANDEM MASS SPECTROMETRY; TUMOR CELLS, CULTURED; VIMENTIN;

EID: 70149101679     PISSN: 08854513     EISSN: None     Source Type: Journal    
DOI: 10.1042/BA20090007     Document Type: Article

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