Expression, refolding, purification, and bioactivity of recombinant bifunctional protein, hIL-2/GM-CSF

Protein Expression and Purification

Volumn 39, Issue 2, 2005, Pages 131-136

  Wang, Qi Rui ^a   Ma, Li ^a   Zhou, Ming Qian ^a   Liu, Nu Yun ^a   Jing, Shen Rong ^b   Zou, Quan Ming ^b   Wang, Xiao Ning ^a  

^a SOUTHERN MEDICAL UNIVERSITY   (China)

^b CHONGQING MEDICAL UNIVERSITY   (China)

Author keywords

Bifunctional molecule; Bioactivity; Expression; Granulocyte macrophage colony stimulating factor; Interleukin 2; Purification; Renaturation

Indexed keywords

ENDOTOXIN; GRANULOCYTE MACROPHAGE COLONY STIMULATING FACTOR; HISTIDINE; HYBRID PROTEIN; INTERLEUKIN 2; ISOPROPYL THIOGALACTOSIDE;

ARTICLE; CELL INCLUSION; CELL PROLIFERATION; CHEMISTRY; DRUG EFFECT; ESCHERICHIA COLI; GENE VECTOR; GENETICS; GROWTH, DEVELOPMENT AND AGING; ION EXCHANGE CHROMATOGRAPHY; ISOLATION AND PURIFICATION; LIMULUS LYSATE TEST; METABOLISM; PLASMID; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN RENATURATION; WESTERN BLOTTING;

BLOTTING, WESTERN; CELL PROLIFERATION; CHROMATOGRAPHY, ION EXCHANGE; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENDOTOXINS; ESCHERICHIA COLI; GENETIC VECTORS; GRANULOCYTE-MACROPHAGE COLONY-STIMULATING FACTOR; HISTIDINE; INCLUSION BODIES; INTERLEUKIN-2; ISOPROPYL THIOGALACTOSIDE; LIMULUS TEST; PLASMIDS; PROTEIN RENATURATION; RECOMBINANT FUSION PROTEINS;

ESCHERICHIA COLI;

EID: 11944249399     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2004.09.017     Document Type: Article

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