Vascular smooth muscle cells promote endothelial cell adhesion via microtubule dynamics and activation of paxillin and the extracellular signal-regulated kinase (ERK) pathway in a co-culture system

European Journal of Cell Biology

Volumn 88, Issue 11, 2009, Pages 701-709

  Wang, Yan Hua ^a   Yan, Zhi Qiang ^a   Shen, Bao Rong ^a   Zhang, Lu ^a   Zhang, Ping ^a   Jiang, Zong Lai ^a  

^a SHANGHAI JIAO TONG UNIVERSITY   (China)

Author keywords

Co culture; Endothelial cell; ERK; Paxillin; Trichostatin A; Vascular smooth muscle cell

Indexed keywords

MITOGEN ACTIVATED PROTEIN KINASE; PAXILLIN; TRICHOSTATIN A;

ARTICLE; CELL ADHESION; COCULTURE; CONTROLLED STUDY; ENDOTHELIUM CELL; ENZYME ACTIVATION; FOCAL ADHESION; HUMAN; HUMAN CELL; MICROTUBULE ASSEMBLY; MOLECULAR MECHANICS; PRIORITY JOURNAL; SMOOTH MUSCLE FIBER; VASCULAR SMOOTH MUSCLE;

CELL ADHESION; COCULTURE TECHNIQUES; ENDOTHELIAL CELLS; EXTRACELLULAR SIGNAL-REGULATED MAP KINASES; HUMANS; MAP KINASE SIGNALING SYSTEM; MICROTUBULES; MUSCLE, SMOOTH, VASCULAR; PAXILLIN; UMBILICAL CORD;

EID: 69949109540     PISSN: 01719335     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ejcb.2009.06.001     Document Type: Article

References (39)

1. Ballestrem C., Wehrle-Haller B., Hinz B., and Imhof B.A. Actin-dependent lamellipodia formation and microtubule-dependent tail retraction control directed cell migration. Mol. Biol. Cell 11 (2000) 2999-3012
2. Brown M.C., and Turner C.E. Roles for the tubulin- and PTP-PEST-binding paxillin LIM domains in cell adhesion and motility. Int. J. Biochem. Cell Biol. 34 (2002) 855-863
3. Brown M.C., Perrotta J.A., and Turner C.E. Identification of LIM3 as the principal determinant of paxillin focal adhesion localization and characterization of a novel motif on paxillin directing vinculin and focal adhesion kinase binding. J. Cell Biol. 135 (1996) 1109-1123
4. Burridge K., Turner C.E., and Romer L.H. Tyrosine phosphorylation of paxillin and pp125FAK accompanies cell adhesion to extracellular matrix: a role in cytoskeletal assembly. J. Cell Biol. 119 (1992) 893-903
5. Cheng C., Liu H., Ge H., Qian J., Qin J., Sun L., and Shen A. Essential role of Src suppressed C kinase substrates in endothelial cell adhesion and spreading. Biochem. Biophys. Res. Commun. 358 (2007) 342-348
6. Daniels R.H., and Bokoch G.M. p21-activated protein kinase: a crucial component of morphological signaling?. Trends Biochem. Sci. 24 (1999) 350-355
7. Danowski B.A. Fibroblast contractility and actin organization are stimulated by microtubule inhibitors. J. Cell Sci. 13 (1989) 255-266
8. Nichilo M.O., and Yamada K.M. Integrin alpha v beta 5-dependent serine phosphorylation of paxillin in cultured human macrophages adherent to vitronectin. J. Biol. Chem. 271 (1996) 11016-11022
9. Finkelstein E., Chang W., Chao P.H., Gruber D., Minden A.D., Hung C.T., and Bulinski J.C. Roles of microtubules, cell polarity, and adhesion in electric field-mediated motility of 3T3 fibroblasts. J. Cell Sci. 117 (2004) 1533-1545
10. Gallant N.D., Michael K.E., and García A.J. Cell adhesion strengthening: contributions of adhesive area, integrin binding, and focal adhesion assembly. Mol. Biol. Cell 16 (2005) 4329-4340
11. Geiger B., and Bershadsky A. Assembly and mechanosensory function of focal contacts. Curr. Opin. Cell Biol. 13 (2001) 584-592
12. Giehl K., Graness A., and Goppelt-Struebe M. The small GTPase Rac-1 is a regulator of mesangial cell morphology and thrombospondin-1 expression. Am. J. Physiol. Renal Physiol 294 (2008) 407-413
13. Huang Y., Sook-Kim M., and Ratovitski E. Midkine promotes tetraspanin-integrin interaction and induces FAK-Stat1α pathway contributing to migration/invasiveness of human head and neck squamous cell carcinoma cells. Biochem. Biophys. Res. Commun. 377 (2008) 474-478
14. Ivanov D., Philippova M., Tkachuk V., Erne P., and Resink T. Cell adhesion molecule T-cadherin regulates vascular cell adhesion, phenotype and motility. Exp. Cell Res. 293 (2004) 207-218
15. Kappert K., Schmidt G., Doerr G., Wollert-Wulf B., Fleck E., and Graf K. Angiotensin II and PDGF-BB stimulate beta(1)-integrin-mediated adhesion and spreading in human VSMCs. Hypertension 35 (2000) 255-261
16. Kaverina I., Krylyshkina O., Gimona M., Beningo K., Wang Y.L., and Small J.V. Enforced polarisation and locomotion of fibroblasts lacking microtubules. Curr. Biol. 10 (2000) 739-742
17. Kirchner J., Kam Z., Tzur G., Bershadsky A.D., and Geiger B. Live-cell monitoring of tyrosine phosphorylation in focal adhesions following microtubule disruption. J. Cell Sci. 116 (2003) 975-986
18. Kroboth K., Newton I.P., Kita K., Dikovskaya D., Zumbrunn J., Waterman-Storer C.M., and Näthke I.S. Lack of adenomatous polyposis coli protein correlates with a decrease in cell migration and overall changes in microtubule stability. Mol. Biol. Cell 18 (2007) 910-918
19. Ku H., and Meier K.E. Phosphorylation of paxillin via the ERK mitogen-activated protein kinase cascade in EL4 thymoma cells. J. Biol. Chem. 275 (2000) 11333-11340
20. Ladwein M., and Rottner K. On the Rho'd: the regulation of membrane protrusions by Rho-GTPases. FEBS Lett. 582 (2008) 2066-2074
21. Lee M.S., Kim Y.B., Lee S.Y., Kim J.G., Kim S.H., Ye S.K., and Lee J.W. Integrin signaling and cell spreading mediated by phorbol 12-myristate 13-acetate treatment. J. Cell. Biochem. 99 (2006) 88-95
22. Liu Z.X., Yu C.F., Nickel C., Thomas S., and Cantley L.G. Hepatocyte growth factor induces ERK-dependent paxillin phosphorylation and regulates paxillin-focal adhesion kinase association. J. Biol. Chem. 277 (2002) 10452-10458
23. Mandato C.A., Benink H.A., and Bement W.M. Microtubule-actomyosin interactions in cortical flow and cytokinesis. Cell Motil. Cytoskeleton 45 (2000) 87-92
24. Pullikuth A., McKinnon E., Schaeffer H.J., and Catling A.D. The MEK1 scaffolding protein MP1 regulates cell spreading by integrating PAK1 and Rho signals. Mol. Cell. Biol. 25 (2005) 5119-5133
25. Reinhart-King C.A., Dembo M., and Hammer D.A. The dynamics and mechanics of endothelial cell spreading. Biophys. J. 89 (2005) 676-689
26. Rozengurt E. Convergent signalling in the action of integrins, neuropeptides, growth factors and oncogenes. Cancer Surv. 24 (1995) 81-96
27. Sabe H., Hata A., Okada M., Nakagawa H., and Hanafusa H. Analysis of the binding of the Src homology 2 domain of Csk to tyrosine-phosphorylated proteins in the suppression and mitotic activation of c-Src. Proc. Natl. Acad. Sci. USA 91 (1994) 3984-3988
28. Small J.V., Geiger B., Kaverina I., and Bershadsky A. How do microtubules guide migrating cells?. Nat. Rev. Mol. Cell Biol. 3 (2002) 957-964
29. 2+ pathways. Cell. Mol. Life Sci. 65 (2008) 2933-2942
30. Teis D., Wunderlich W., and Huber L.A. Localization of the MP1-MAPK scaffold complex to endosomes is mediated by p14 and required for signal transduction. Dev. Cell 3 (2002) 803-814
31. Turner C.E. Paxillin. Int. J. Biochem. Cell Biol. 30 (1998) 955-959
32. Turner C.E. Paxillin interactions. J. Cell Sci. 113 (2000) 4139-4140
33. Ujihara Y., Miyazaki H., and Wada S. Morphological study of fibroblasts treated with cytochalasin D and colchicine using a confocal laser scanning microscopy. J. Physiol. Sci. 58 (2008) 499-506
34. Vasiliev J.M., and Gelfand I.M. Effects of colcemid on morphogenetic processes and locomotion of fibroblasts. In: Goldman R., Pollard T., and Rosenbaum J. (Eds). Cell Motility (1976), Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York 279-304
35. Walsh C.J. The role of actin, actomyosin and microtubules in defining cell shape during the differentiation of Naegleria amebae into flagellates. Eur. J. Cell Biol. 86 (2007) 85-98
36. Wang H.Q., Bai L., Shen B.R., Yan Z.Q., and Jiang Z.L. Coculture with endothelial cells enhances vascular smooth muscle cell adhesion and spreading via activation of beta1-integrin and phosphatidylinositol 3-kinase/Akt. Eur. J. Cell Biol. 86 (2007) 51-62
37. Wehrle-Haller B., and Imhof B.A. Actin, microtubules and focal adhesion dynamics during cell migration. Int. J. Biochem. Cell Biol. 35 (2003) 39-50
38. Zhai Y., Kronebusch P.J., Simon P.M., and Borisy G.G. Microtubule dynamics at the G2/M transition: abrupt breakdown of cytoplasmic microtubules at nuclear envelope breakdown and implications for spindle morphogenesis. J. Cell Biol. 135 (1996) 201-214
39. Zhou X., Li J., and Kucik D.F. The microtubule cytoskeleton participates in control of beta2 integrin avidity. J. Biol. Chem. 276 (2001) 44762-44769