Spodoptera frugiperda X-tox protein, an immune related defensin rosary, has lost the function of ancestral defensins

PLoS ONE

Volumn 4, Issue 8, 2009, Pages

  Destoumieux Garzón, Delphine ^a,b   Brehelin, Michel ^b,c   Bulet, Philippe ^d   Boublik, Yvan ^e   Girard, Pierre Alain ^b,c   Baghdiguian, Stephen ^b   Zumbihl, Robert ^b,c   Escoubas, Jean Michel ^b,c  

^a UNIVERSITÉ MONTPELLIER 2   (France)

^b UNIV MONTPELLIER   (France)

^c University of Montpellier II   (France)

^d UNIV GRENOBLE ALPES   (France)

^e CNRS   (France)

Author keywords

[No Author keywords available]

Indexed keywords

DEFENSIN; INSECT PROTEIN; PROTEIN 11 TOX; PROTEIN X TOX; UNCLASSIFIED DRUG;

ANIMAL CELL; ANTIMICROBIAL ACTIVITY; ARTICLE; ASSAY; CELLULAR DISTRIBUTION; CONTROLLED STUDY; GRANULOCYTE; HEMOLYMPH; IMMUNOCHEMISTRY; INNATE IMMUNITY; LEPIDOPTERA; MOLECULAR EVOLUTION; NONHUMAN; PHAGOCYTOSIS; PLASMA CELL; PROTEIN EXPRESSION; PROTEIN SECRETION; ANIMAL; ARMYWORM; CELL FRACTIONATION; CONFOCAL MICROSCOPY; ENZYME LINKED IMMUNOSORBENT ASSAY; FLUORESCENT ANTIBODY TECHNIQUE; GENE EXPRESSION REGULATION; GENETICS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; IMMUNOLOGY; METABOLISM; PHYSIOLOGY; WESTERN BLOTTING;

BACTERIA (MICROORGANISMS); EUKARYOTA; HEXAPODA; LEPIDOPTERA; SPODOPTERA FRUGIPERDA;

ANIMALS; BLOTTING, WESTERN; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; ENZYME-LINKED IMMUNOSORBENT ASSAY; FLUORESCENT ANTIBODY TECHNIQUE; GENE EXPRESSION REGULATION; INSECT PROTEINS; MICROSCOPY, CONFOCAL; PHAGOCYTOSIS; SPODOPTERA; SUBCELLULAR FRACTIONS;

EID: 69449103610     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0006795     Document Type: Article

References (31)

1. Ferrandon D, Imler JL, Hetru C, Hoffmann JA (2007) The Drosophila systemic immune response: sensing and signalling during bacterial and fungal infections. Nat Rev Immunol 7: 862-874.
2. Lemaitre B, Hoffmann J (2007) The Host Defense of Drosophila melanogaster. Annu Rev Immunol.
3. Evans JD, Aronstein K, Chen YP, Hetru C, Imler JL, et al. (2006) Immune pathways and defence mechanisms in honey bees Apis mellifera. Insect Mol Biol 15: 645-656.
4. Tanaka H, Ishibashi J, Fujita K, Nakajima Y, Sagisaka A, et al. (2008) A genome-wide analysis of genes and gene families involved in innate immunity of Bombyx mori. Insect Biochem Mol Biol.
5. Waterhouse RM, Kriventseva EV, Meister S, Xi Z, Alvarez KS, et al. (2007) Evolutionary dynamics of immune-related genes and pathways in disease-vector mosquitoes. Science 316: 1738-1743.
6. Zou Z, Evans JD, Lu Z, Zhao P, Williams M, et al. (2007) Comparative genomic analysis of the Tribolium immune system. Genome Biol 8: R177.
7. Sun S-C, Lindström I, Boman HG, Faye I, Schmidt O (1990) Hemolin: an insect-immune protein belonging to the immunoglobulin superfamily. Science 250: 1729-1732.
8. Yu XQ, Kanost MR (2002) Binding of hemolin to bacterial lipopolysaccharide and lipoteichoic acid. An immunoglobulin superfamily member from insects as a pattern-recognition receptor. Eur J Biochem 269: 1827-1834.
9. Eleftherianos I, Gokcen F, Felfoldi G, Millichap PJ, Trenczek TE, et al. (2007) The immunoglobulin family protein Hemolin mediates cellular immune responses to bacteria in the insect Manduca sexta. Cell Microbiol 9: 1137-1147.
10. Girard PA, Boublik Y, Wheat CW, Volkoff AN, Cousserans F, et al. (2008) X-tox: An atypical defensin derived family of immune-related proteins specific to Lepidoptera. Dev Comp Immunol 32: 575-584.
11. Cornet B, Bonmatin JM, Hetru C, Hoffmann JA, Ptak M, et al. (1995) Refined three-dimensional solution structure of insect defensin A. Structure 3: 435-448.
12. Seitz V, Clermont A, Wedde M, Hummel M, Vilcinskas A, et al. (2003) Identification of immunorelevant genes from greater wax moth (Galleria mellonella) by a subtractive hybridization approach. Dev Comp Immunol 27: 207-215.
13. Taniai K, Lee JH, Lee IH (2006) Bombyx mori cell line as a model of immunesystem organs. Insect Mol Biol 15: 269-279.
14. Ribeiro C, Vignes M, Brehelin M (2003) Xenorhabdus nematophila (enterobacteriacea) secretes a cation-selective calcium-independent porin which causes vacuolation of the rough endoplasmic reticulum and cell lysis. J Biol Chem 278: 3030-3039.
15. Sicard M, Brugirard-Ricaud K, Pages S, Lanois A, Boemare NE, et al. (2004) Stages of Infection during the Tripartite Interaction between Xenorhabdus nematophila, Its Nematode Vector, and Insect Hosts. Appl Environ Microbiol 70: 6473-6480.
16. Hetru C, Bulet P (1997) Strategies for the isolation and characterization of antimicrobial peptides of invertebrates. Methods in Molecular Biology 78: 35-49.
17. Nardi JB, Pilas B, Ujhelyi E, Garsha K, Kanost MR (2003) Hematopoietic organs of Manduca sexta and hemocyte lineages. Dev Genes Evol 213: 477-491.
18. Brehélin M, Zachary D (1986) Insect haemocytes: a new classification to rule out the controversy. In: Brehélin M, ed. Immunity in Invertebrates. Berlin: Springer. pp 36-48.
19. Ribeiro C, Brehelin M (2006) Insect haemocytes: what type of cell is that? J Insect Physiol 52: 417-429.
20. Lamberty M, Ades S, Uttenweiler-Joseph S, Brookhart G, Bushey D, et al. (1999) Insect immunity. Isolation from the lepidopteran Heliothis virescens of a novel insect defensin with potent antifungal activity. J Biol Chem 274: 9320-9326.
21. Ganz T (2003) Defensins: antimicrobial peptides of innate immunity. Nat Rev Immunol 3: 710-720.
22. Mitta G, Vandenbulcke F, Hubert F, Roch P (1999) Mussel defensins are synthesised and processed in granulocytes then released into the plasma after bacterial challenge. J Cell Sci 112: 4233-4242.
23. Destoumieux D, Munoz M, Cosseau C, Rodriguez J, Bulet P, et al. (2000) Penaeidins, antimicrobial peptides with chitin-binding activity, are produced and stored in shrimp granulocytes and released after microbial challenge [In Process Citation]. J Cell Sci 113: 461-469.
24. Shigenaga T, Muta T, Toh Y, Tokunaga F, Iwanaga S (1990) Antimicrobial tachyplesin peptide precursor. cDNA cloning and cellular localization in the horseshoe crab (Tachypleus tridentatus). J Biol Chem 265: 21350-21354.
25. Lamberty M, Zachary D, Lanot R, Bordereau C, Robert A, et al. (2001) Insect immunity. Constitutive expression of a cysteine-rich antifungal and a linear antibacterial peptide in a termite insect. J Biol Chem 276: 4085-4092.
26. Bevins CL, Zasloff M (1990) Peptides from frog skin. Annu Rev Biochem 59: 395-414.
27. Casteels-Josson K, Capaci T, Casteels P, Tempst P (1993) Apidaecin multipeptide precursor structure: a putative mechanism for amplification of the insect antibacterial response. Embo J 12: 1569-1578.
28. Shelby KS, Popham HJ (2009) Analysis of ESTs generated from immunestimulated hemocytes of larval Heliothis virescens. J Invertebr Pathol 101(2): 86-95.
29. Cytrynska M, Mak P, Zdybicka-Barabas A, Suder P, Jakubowicz T (2007) Purification and characterization of eight peptides from Galleria mellonella immune hemolymph. Peptides 28: 533-546.
30. Lee YS, Yun EK, Jang WS, Kim I, Lee JH, et al. (2004) Purification, cDNA cloning and expression of an insect defensin from the great wax moth, Galleria mellonella. Insect Mol Biol 13: 65-72.
31. Volkoff AN, Rocher J, d'Alencon E, Bouton M, Landais I, et al. (2003) Characterization and transcriptional profiles of three Spodoptera frugiperda genes encoding cysteine-rich peptides. A new class of defensin-like genes from lepidopteran insects? Gene 319: 43-53.