Identification of immunorelevant genes from greater wax moth (Galleria mellonella) by a subtractive hybridization approach

Developmental and Comparative Immunology

Volumn 27, Issue 3, 2003, Pages 207-215

  Seitz, V ^a   Clermont, A ^b   Wedde, M ^c   Hummel, M ^b   Vilcinskas, A ^c   Schlatterer, K ^b   Podsiadlowski, L ^b  

^a MAX PLANCK INSTITUTE FOR MOLECULAR GENETICS   (Germany)

^b FREIE UNIVERSITÄT BERLIN   (Germany)

^c UNIVERSITY OF POTSDAM   (Germany)

Author keywords

Galleria mellonella; Gloverin; Innate immunity; Lipopolysaccharide; Peptidoglycan recognition protein; Real time PCR; Subtractive hybridization; Toxin 2

Indexed keywords

BACTERIUM LIPOPOLYSACCHARIDE; COMPLEMENTARY DNA; DEFENSIN; GLOVERIN; PEPTIDOGLYCAN; PROTEIN; RNA BINDING PROTEIN; TOXIN; TOXIN 2; TRANSFERRIN; TYROSINE 3 MONOOXYGENASE; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; BACTERIAL INFECTION; BLOOD CELL; CONTROLLED STUDY; HOST RESISTANCE; HYBRIDIZATION; IMMUNITY; IMMUNOMODULATION; MOTH; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN DOMAIN;

ARTHROPODA; BACTERIA (MICROORGANISMS); GALLERIA MELLONELLA; LEPIDOPTERA;

EID: 0037363719     PISSN: 0145305X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0145-305X(02)00097-6     Document Type: Article

References (43)

1. Irving P., Troxler L., Heuer T.S., Belvin M., Kopczynski C., Reichhart J.M., Hoffmann J.A., Hetru C. A genome-wide analysis of immune responses in Drosophila. Proc Natl Acad Sci USA. 98:(26):2001;15119-15124.
2. Hultmark D., Engström A., Andersson K., Steiner H., Bennich H., Boman H.G. Insect immunity. Attacins, a family of antibacterial proteins from Hyalophora cecropia. EMBO J. 2:(4):1983;571-576.
3. Sun S.C., Lindström I., Boman H.G., Faye I., Schmidt O. Hemolin: an insect-immune protein belonging to the immunoglobulin superfamily. Science. 250:(4988):1990;1729-1732.
4. Kanost M.R., Zepp M.K., Ladendorff N.E., Andersson L.A. Isolation and characterization of a hemocyte aggregation inhibitor from hemolymph of Manduca sexta larvae. Arch Insect Biochem Physiol. 27:(2):1994;123-136.
5. Sumida M., Ichimori H., Johchi S., Takaoka A., Yuhki T., Mori H., Matsubara F. Antibacterial activity inducible in the haemolymph of the silkworm, Bombyx mori, by injection of formalin-treated Escherichia coli K-12 during the fifth larval instar and pharate adult development. Comp Biochem Physiol B. 101:(1-2):1992;165-171.
6. Phipps D.J., Chadwick J.S., Aston W.P. Gallysin-1, an antibacterial protein isolated from hemolymph of Galleria mellonella. Dev Comp Immunol. 18:(1):1994;13-23.
7. De Verno P.J., Chadwick J.S., Aston W.P., Dunphy G.B. The in vitro generation of an antibacterial activity from the fat body and hemolymph of non-immunized larvae of Galleria mellonella. Dev Comp Immunol. 8:(3):1984;537-546.
8. Beresford P.J., Basinski-Gray J.M., Chiu J.K., Chadwick J.S., Aston W.P. Characterization of hemolytic and cytotoxic Gallysins: a relationship with arylphorins. Dev Comp Immunol. 21:(3):1997;253-266.
9. Wedde M., Weise C., Kopacek P., Franke P., Vilcinskas A. Purification and characterization of an inducible metalloprotease inhibitor from the hemolymph of greater wax moth larvae, Galleria mellonella. Eur J Biochem. 255:(3):1998;535-543.
10. Frobius A.C., Kanost M.R., Gotz P., Vilcinskas A. Isolation and characterization of novel inducible serine protease inhibitors from larval hemolymph of the greater wax moth Galleria mellonella. Eur J Biochem. 267:(7):2000;2046-2053.
11. Kopacek P., Weise C., Götz P. The prophenoloxidase from the wax moth Galleria mellonella: purification and characterization of the proenzyme. Insect Biochem Mol Biol. 25:(10):1995;1081-1091.
12. Diatchenko L., Lau Y.F., Campbell A.P., Chenchik A., Moqadam F., Huang B., Lukyanov S., Lukyanov K., Guskaya N., Sverdlov E.D., Siebert P.D. Suppression subtractive hybridization: a method for generating differentially regulated or tissue-specific cDNA probes and libraries. Proc Natl Acad Sci USA. 93:(12):1996;6025-6030.
13. Gurskaya N.G., Diatchenko L., Chenchik A., Siebert P.D., Khaspekov G.L., Lukyanov K.A., Vagner L.L., Ermolaeva O.D., Lukyanov S.A., Sverdlov E.D. Equalizing cDNA subtraction based on selective suppression of polymerase chain reaction: cloning of Jurkat cell transcripts induced by phytohemaglutinin and phorbol 12-myristate 13-acetate. Anal Biochem. 240:(1):1996;90-97.
14. Vilcinskas A., Matha V., Göetz P. Inhibition of phagocytic activity of plasmatocytes isolated from Galleria mellonella by entomogenous fungi and their secondary metabolites. J Ins Physiol. 43:1997;475-483.
15. Morrison T.B., Weis J.J., Wittwer C.T. Quantification of low-copy transcripts by continuous SYBR Green I monitoring during amplification. Biotechniques. 24:(6):1998;954-958. See also p. 960 and 962.
16. Kumagai T., Awai M., Okada S. Mobilization of iron and iron-related proteins in rat spleen after intravenous injection of lipopolysaccharides (LPS). Pathol Res Pract. 188:(7):1992;931-941.
17. Stolzenberg E.D., Anderson G.M., Ackermann M.R., Whitlock R.H., Zasloff M. Epithelial antibiotic induced in states of disease. Proc Natl Acad Sci USA. 94:(16):1997;8686-8690.
18. Ganz T., Lehrer R.I. Antimicrobial peptides of leukocytes. Curr Opin Hematol. 4:(1):1997;53-58.
19. Kwon T.H., Kim M.S., Choi H.W., Joo C.H., Cho M.Y., Lee B.L. A masquerade-like serine proteinase homologue is necessary for phenoloxidase activity in the coleopteran insect, Holotrichia diomphalia larvae. Eur J Biochem. 267:(20):2000;6188-6196.
20. Kol A., Lichtman A.H., Finberg R.W., Libby P., Kurt-Jones E.A. Cutting edge: heat shock protein (HSP) 60 activates the innate immune response: CD14 is an essential receptor for HSP60 activation of mononuclear cells. J Immunol. 164:(1):2000;13-17.
21. Axen A., Carlsson A., Engström A., Bennich H. Gloverin, an antibacterial protein from the immune hemolymph of Hyalophora pupae. Eur J Biochem. 247:(2):1997;614-619.
22. Ochiai M., Ashida M. A pattern recognition protein for peptidoglycan. Cloning the cDNA and the gene of the silkworm, Bombyx mori. J Biol Chem. 274:(17):1999;11854-11858.
23. Yoshida H., Kinoshita K., Ashida M. Purification of a peptidoglycan recognition protein from hemolymph of the silkworm, Bombyx mori. J Biol Chem. 271:(23):1996;13854-13860.
24. Yoshida H., Ochiai M., Ashida M. Beta-1,3-glucan receptor and peptidoglycan receptor are present as separate entities within insect prophenoloxidase activating system. Biochem Biophys Res Commun. 141:(3):1986;1177-1184.
25. Kang D., Liu G., Lundström A., Gelius E., Steiner H. A peptidoglycan recognition protein in innate immunity conserved from insects to humans. Proc Natl Acad Sci USA. 95:(17):1998;10078-10082.
26. Yoshimura A., Lien E., Ingalls R.R., Tuomanen E., Dziarski R., Golenbock D. Cutting edge: recognition of Gram-positive bacterial cell wall components by the innate immune system occurs via Toll-like receptor 2. J Immunol. 163:(1):1999;1-5.
27. Werner T., Liu G., Kang D., Ekengren S., Steiner H., Hultmark D. A family of peptidoglycan recognition proteins in the fruit fly Drosophila melanogaster. Proc Natl Acad Sci USA. 97:(25):2000;13772-13777.
28. Kim Y.J., Zuo P., Manley J.L., Baker B.S. The Drosophila RNA-binding protein RBP1 is localized to transcriptionally active sites of chromosomes and shows a functional similarity to human splicing factor ASF/SF2. Genes Dev. 6:(12B):1992;2569-2579.
29. Birman S., Morgan B., Anzivino M., Hirsh J. A novel and major isoform of tyrosine hydroxylase in Drosophila is generated by alternative RNA processing. J Biol Chem. 269:(42):1994;26559-26567.
30. Janeway C.A. Jr. Approaching the asymptote? Evolution and revolution in immunology. Cold Spring Harb Symp Quant Biol. 54:(Pt 1):1989;1-13.
31. Medzhitov R., Janeway C.A. Jr. Innate immune recognition and control of adaptive immune responses. Semin Immunol. 10:(5):1998;351-353.
32. Medzhitov R., Preston-Hurlburt P., Janeway C.A. Jr. A human homologue of the Drosophila Toll protein signals activation of adaptive immunity. Nature. 388:(6640):1997;394-397.
33. Weinberg E.D. The development of awareness of iron-withholding defense. Perspect Biol Med. 36:(2):1993;215-221.
34. Nichol H., Law J.H., Winzerling J.J. Iron metabolism in insects. Annu Rev Entomol. 47:2002;535-559.
35. Selisko B., Garcia C., Becerril B., Gomez-Lagunas F., Garay C., Possani L.D. Cobatoxins 1 and 2 from Centruroides noxius Hoffmann constitute a subfamily of potassium-channel-blocking scorpion toxins. Eur J Biochem. 254:(3):1998;468-479.
36. Martin B.M., Ramirez A.N., Gurrola G.B., Nobile M., Prestipino G., Possani L.D. Novel K(+)-channel-blocking toxins from the venom of the scorpion Centruroides limpidus Karsch. Biochem J. 304:(Pt 1):1994;51-56.
37. Lippens G., Najib J., Wodak S.J., Tartar A. NMR sequential assignments and solution structure of chlorotoxin, a small scorpion toxin that blocks chloride channels. Biochemistry. 34:(1):1995;13-21.
38. Zhu S., Li W., Jiang D., Zeng X. Evidence for the existence of insect defensin-like peptide in scorpion venom. IUBMB Life. 50:(1):2000;57-61.
39. Cornet B., Bonmatin J.M., Hetru C., Hoffmann J.A., Ptak M., Vovelle F. Refined three-dimensional solution structure of insect defensin A. Structure. 3:(5):1995;435-448.
40. Landon C., Sodano P., Hetru C., Hoffmann J., Ptak M. Solution structure of drosomycin, the first inducible antifungal protein from insects. Protein Sci. 6:(9):1997;1878-1884.
41. Bontems F., Roumestand C., Gilquin B., Menez A., Toma F. Refined structure of charybdotoxin: common motifs in scorpion toxins and insect defensins. Science. 254:(5037):1991;1521-1523.
42. Ehret-Sabatier L., Loew D., Goyffon M., Fehlbaum P., Hoffmann J.A., van Dorsselaer A., Bulet P. Characterization of novel cysteine-rich antimicrobial peptides from scorpion blood. J Biol Chem. 271:(47):1996;29537-29544.
43. Dimarcq J.L., Bulet P., Hetru C., Hoffmann J. Cysteine-rich antimicrobial peptides in invertebrates. Biopolymers. 47:(6):1998;465-477.