A single amino acid change (Y318F) in the L-arabitol dehydrogenase (LadA) from Aspergillus niger results in a significant increase in affinity for D-sorbitol

BMC Microbiology

Volumn 9, Issue , 2009, Pages

  Rutten, Lucy ^a   Ribot, Cecile ^a,b,c   Trejo Aguilar, Blanca ^a   Wösten, Han A B ^a   De Vries, Ronald P ^a,d  

^a UTRECHT UNIVERSITY   (Netherlands)

^b BAYER AG   (Germany)

^c Campus International de Baillarguet   (France)

^d CBS KNAW FUNGAL BIODIVERSITY CENTRE   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

ALCOHOL DEHYDROGENASE; AMINO ACID; ARABINITOL; IDITOL DEHYDROGENASE; ALDEHYDE REDUCTASE; FUNGAL DNA; SORBITOL; XYLULOSE REDUCTASE;

ARTICLE; ASPERGILLUS NIGER; ENZYME ACTIVATION; ENZYME ACTIVITY; ENZYME SPECIFICITY; FUNGUS; MUTANT; MUTATION; NONHUMAN; NUCLEOTIDE SEQUENCE; PHYLOGENY; PROTEIN DOMAIN; AMINO ACID SUBSTITUTION; CHEMICAL STRUCTURE; DNA SEQUENCE; ENZYMOLOGY; FUNGAL GENE; GENETICS; METABOLISM; SITE DIRECTED MUTAGENESIS;

ASPERGILLUS NIGER; FUNGI;

AMINO ACID SUBSTITUTION; ASPERGILLUS NIGER; D-XYLULOSE REDUCTASE; DNA, FUNGAL; GENES, FUNGAL; L-IDITOL 2-DEHYDROGENASE; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; MUTATION; PHYLOGENY; SEQUENCE ANALYSIS, DNA; SORBITOL; SUBSTRATE SPECIFICITY; SUGAR ALCOHOL DEHYDROGENASES;

EID: 69449092483     PISSN: 14712180     EISSN: 14712180     Source Type: Journal    
DOI: 10.1186/1471-2180-9-166     Document Type: Article

References (22)

1. Aspergillus enzymes involved in degradation of plant cell wall polysaccharides. RP de Vries J Visser, Microb Mol Biol Rev 2001 65 497 522
2. L-arabinose and D-xylose catabolism in Aspergillus niger. CFB Witteveen R Busink P van de Vondervoort C Dijkema K Swart J Visser, J Gen Microbiol 1989 135 2163 2171
3. Isolation and characterization of two specific regulatory Aspergillus niger mutants shows antagonistic regulation of arabinan and xylan metabolism. MJ de Groot PJI van de Vondervoort RP de Vries PA vanKuyk GJ Ruijter J Visser, Microbiol 2003 149 1183 1191
4. Metabolic control analysis of Aspergillus niger L-arabinose catabolism. MJ de Groot W Prathumpai J Visser GJ Ruijter, Biotechnol Prog 2005 21 1610 1616
5. Regulation and control of L-arabinose catabolism in Aspergillus niger. MJL de Groot, PhD thesis Wageningen University, Microbiology 2005 http://www.library.wur.nl/wda/dissertations/dis3819.pdf
6. Characterisation of an Aspergillus nidulans L-arabitol dehydrogenase mutant. RP de Vries MJ Flipphi CF Witteveen J Visser, FEMS Microbiol Lett 1994 123 83 90
7. The metabolic role and evolution of L-arabinitol 4-dehydrogenase of Hypocrea jecorina. M Pail T Peterbauer B Seiboth C Hametner I Druzhinina CP Kubicek, Eur J Biochem 2004 271 1864 1872
8. Cloning and expression of a fungal L-arabinitol 4-dehydrogenase gene. P Richard J Londesborough M Putkonen N Kalkkinen, J Biol Chem 2001 276 40631 40637
9. D-Xylose metabolism in Hypocrea jecorina: Loss of the xylitol dehydrogenase step can be partially compensated for by lad1-encoded L-arabinitol-4-dehydrogenase. B Seiboth L Hartl M Pail CP Kubicek, Eukaryotic Cell 2003 2 867 875
10. The Aspergillus niger D-xylulose kinase gene is co-expressed with genes encoding arabinan degrading enzymes and is essential for growth on arabinose and xylose. PA vanKuyk MJ de Groot GJ Ruijter RP de Vries J Visser, Eur J Biochem 2001 268 5414 5423
11. Isolation and characterisation of two xylitol dehydrogenases from Aspergillus niger. CFB Witteveen F Weber R Busink J Visser, Microbiol 1994 140 1679 1685
12. X-ray crystallographic and kinetic studies of human sorbitol dehydrogenase. TA Pauly JL Ekstrom DA Beebe B Chrunyk D Cunningham M Griffor A Kamath SE Lee R Madura D Mcguire, Structure 2003 11 1071 1085
13. Crystal structure of sorbitol dehydrogenase. K Johansson M El-Ahmad C Kaiser H Jörnvall H Eklund J-O Höög S Ramaswamy, Chemico-Biological Interactions 2001 132 351 358
14. Lambda ZAP: a bacteriophage expression vector with in vivo excision properties. JM Short JM Fernandez JA Sorge WD Huse, Nucl Acids Res 1988 16 7583 7600
15. Molecular cloning -a laboratory manual. J Sambrook EF Fritsch T Maniatis, Cold Spring Harbour, N.Y.: Cold Spring Harbour Laboratory 2 1989
16. A comprehensive set of sequence analysis programs for the vax. J Devereux P Haeberli O Smithies, Nucl Acids Res 1984 12 387 395
17. Basic local alignment search tool. SF Altschul W Gish W Miller EW Myers DJ Lipman, J Mol Biol 1990 215 403 410
18. Multiple sequence alignment using ClustalW and ClustalX. JD Thompson TJ Gibson DG Higgins, Curr Protoc Bioinformatics 2002 Chapter 2 Unit 2 3
19. Protein modeling by E-mail. MC Peitsch, Bio/Technol 1995 13 658 660
20. SWISS-MODEL and the Swiss-PdbViewer: An environment for comparative protein modelling. N Guex MC Peitsch, Electrophoresis 1997 18 2714 2723
21. SWISS-MODEL: an automated protein homology-modeling server. T Schwede J Kopp N Guex MC Peitsch, Nucl Acids Res 2003 31 3381 3385
22. The PyMOL molecular graphics system. http://www.pymol.org