Role of the Y134F mutation in cyp51 and overexpression of cyp51 in the sensitivity response of Puccinia triticina to epoxiconazole

Crop Protection

Volumn 28, Issue 10, 2009, Pages 891-897

  Stammler, G ^a   Cordero, J ^a   Koch, A ^a   Semar, M ^a   Schlehuber, S ^a  

^a BASF SE   (Germany)

Author keywords

Cytochrome P450 sterol 14 demethylase enzyme; Demethylation inhibitor; Puccinia triticina; Sensitivity; Triazoles; Wheat leaf rust

Indexed keywords

ENZYME ACTIVITY; FUNGICIDE; GENE EXPRESSION; MUTATION; PESTICIDE RESISTANCE; RUST DISEASE; WHEAT;

PUCCINIA TRITICINA; TRITICUM AESTIVUM;

EID: 69249222646     PISSN: 02612194     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.cropro.2009.05.007     Document Type: Article

References (23)

1. Albertini C., Gredt M., and Leroux P. Polymorphism of 14α-demethylase gene (cyp51) in the Cereal Eyespot fungi Tapesia acuformis and Tapesia yallundae. European Journal of Plant Pathology 109 (2003) 117-128
2. Délye C., Laigret F., and Corio-Costet M.F. A mutation in the 14α-demethylase gene of Uncinula necator that correlates with resistance to a sterol biosynthesis inhibitor. Applied and Environmental Microbiology 63 (1997) 2966-2970
3. Délye C., Bousset L., and Corio-Costet M.F. PCR cloning and detection of point mutations in the eburicol 14 alpha-demethylase (CYP51) gene from Erysiphe graminis f. sp. hordei, a "recalcitrant" fungus. Current Genetics 34 (1998) 399-403
4. FRAC, 2008. Available from: www.frac.info.
5. Hamamoto H., Hasegawa K., Nakaune R., Lee Y.J., Makizumi Y., Akutsu K., and Hibi T. Tandem repeat of a transcriptional enhancer upstream of the sterol 14 alpha-demethylase gene (cyp51) in Penicillium digitatum. Applied and Environmental Microbiology 66 (2000) 3421-3426
6. Hysing S.C., Singh R.P., Huerta J., Merker A., Liljeroth E., and Diaz O. Leaf rust (Puccinia triticina) resistance in wheat (Triticum aestivum) cultivars grown in Northern Europe 1992-2002. Hereditas 143 (2006) 1-14
7. Leroux P., Albertini C., Gautier A., Gredt M., and Walker A.S. Mutations in the CYP51 with changes in sensitivity to sterol 14α-demethylase inhibitors in field isolates of Mycosphaerella graminicola. Pest Management Science 63 (2007) 688-698
8. Livak K.J., and Schmittgen T.D. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods 25 (2001) 402-408
9. Luo C.X., and Schnabel G. The cytochrome P450 lanosterol 14 alpha-demethylase gene is a demethylation inhibitor fungicide resistance determinant in Monilinia fructicola field isolates from Georgia. Applied and Environmental Microbiology 74 (2008) 59-66
10. Lupetti A., Danesi R., Campa M., Del Tacca M., and Kelly S. Molecular basis of resistance to azole antifungals. Trends in Molecular Medicine 2 (2002) 76-81
11. Lyons C.N., and White T.C. Transcriptional analyses of antifungal drug resistance in Candida albicans. Antimicrobial Agents and Chemotherapy 44 (2000) 2296-2303
12. Ma Z., Proffer T.J., Jacobs J.L., and Sundin G.W. Overexpression of the 14 alpha-demethylase target gene (CYP51) mediates fungicide resistance in Blumeriella jaapii. Applied and Environmental Microbiology 72 (2006) 2581-2585
13. Marichal P., Vanden Bossche H., Odds F.C., Nobels G., Warnock D.W., Timmerman V., Van Broeckhoven C., Fay S., and Mose-Larsen P. Molecular biological characterization of an azole resistant Candida glabrata isolates. Antimicrobial Agents and Chemotherapy 41 (1997) 2229-2237
14. Nakaune R., Adachi K., Nawata O., Tomiyama M., Akutsu K., and Hibi T. A novel ATP-Binding cassette transporter involved in multidrug resistance in phytopathogenic fungus Penicillium digitatum. Antimicrobial Agents and Chemotherapy 64 (1998) 3983-3988
15. Pfaffl M.W., Horgan G.W., and Dempfle L. Relative software (REST©) for group-wise comparison and statistical analysis of relative expression results in real-time PCR. Nucleic Acids Research 30 (2002) 1-10
16. Podust L.M., Stojan J., Poulos T.L., and Waterman M.R. Substrate recognition sites in 14α-esterol demethylase from comparative analysis of amino acid sequences of Mycobacterium tuberculosis CYP51. Journal of Inorganic Biochemistry 87 (2001) 227-235
17. Sanglard D., Ischer F., Monod M., and Bille J. Cloning of Candida albicans genes conferring resistance to azole CDR2, a new multidrug ABC transporter. Microbiology 143 (1997) 405-416
18. Schnabel G., and Jones A.L. The 14 α-Demethylase Cyp51A1 gene is overexpressed in Venturia inaequalis strains resistant to myclobutanil. Phytopathology 91 (2001) 102-110
19. Stammler G., Carstensen M., Koch A., Semar M., Strobel D., and Schlehuber S. Frequency of different CYP51-haplotypes of Mycosphaerella graminicola and their impact on epoxiconazole-sensitivity and -field efficacy. Crop Protection 27 (2008) 1148-1456
20. Uesugi Y. Fungicide classes: chemistry, uses and mode of action. In: Hutson D., and Miyamoto J. (Eds). Fungicidal Activity Chemical and Biological Approaches to Plant Protection (1998), John Wiley & Sons, England 20-23
21. Wyand R.A., and Brown J.K. Sequence variation in the CYP51 gene of Blumeria graminis associated with resistance to sterol demethylase inhibiting fungicides. Fungal Genetics and Biology 42 (2005) 726-735
22. Yoshida Y., Aoyama Y., Noshiro M., and Gotoh O. Sterol 14 alpha-demethylase P450 (CYP51) provides a breakthrough for the discussion of the evolution of cytochrome P450 gene superfamily. Biochemical and Biophysical Research Communications 273 (2000) 799-804
23. Zwiers L.H., Stergiopoulos I., Van Nistelrooy J.G., and De Waard M.A. ABC transporters and azole susceptibility in laboratory strains of the wheat pathogen Mycosphaerella graminicola. Antimicrobial Agents and Chemotherapy 46 (2002) 3900-3906