Frequency of different CYP51-haplotypes of Mycosphaerella graminicola and their impact on epoxiconazole-sensitivity and -field efficacy

Crop Protection

Volumn 27, Issue 11, 2008, Pages 1448-1456

  Stammler, G ^a   Carstensen, M ^a   Koch, A ^a   Semar, M ^a   Strobel, D ^a   Schlehuber, S ^a  

^a BASF SE   (Germany)

Author keywords

14 Demethylase; CYP51; Demethylation inhibitor; Epoxiconazole; Field efficacy; Microtitre assay; Mycosphaerella graminicola; Pyrosequencing; Septoria leaf blotch

Indexed keywords

DISEASE CONTROL; DISEASE RESISTANCE; ENZYME ACTIVITY; FUNGUS; GENETIC ENGINEERING; INHIBITION; MUTATION; WHEAT;

EURASIA; EUROPE;

MYCOSPHAERELLA GRAMINICOLA; SEPTORIA; TRITICUM AESTIVUM;

EID: 53249105343     PISSN: 02612194     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.cropro.2008.07.007     Document Type: Article

References (26)

1. Abram M. Effectiveness of triazole fungicides under question. Farmers Weekly 145 10 (2006) 45
2. Abram M. Old fungicide plays a part in beating wheat disease. Farmers Weekly 147 25 (2007) 50-51
3. Abram M. No let-up for triazoles. Farmers Weekly 148 10 (2008) 62
4. Bean, T., 2005. Amino acid alterations in CYP51 contribute toward reduced triazole sensitivities in a UK field population of Mycosphaerella graminicola. In: BCPC International Congress, Crop Science and Technology, Pests and Diseases, pp. 467-470.
5. Bearchell S.J., Fraaije B.A., Shaw M.W., and Fitt B.D.L. Wheat archive links long-term fungal pathogen population dynamics to air pollution. Proceedings of the National Academy of Sciences of the United States of America 102 (2005) 5438-5442
6. Brunner P.C., Stefano F.L., and McDonald B.A. Evolution of the CYP51 gene in Mycosphaerella graminicola: evidence for intragenic recombination and selective replacement. Molecular Plant Pathology 9 (2008) 305-316
7. Clark W.S. Septoria tritici and azole performance. Fungicide resistance: are we winning the battle but losing the war?. Aspects of Applied Biology 78 (2006) 127-132
8. Cools, H.J., Fraaije, B.A., Lucas, J., 2005. Molecular examination of Septoria tritici isolates with reduced sensitivities to triazoles. In: Modern Fungicides and Antifungal Compounds IV, pp. 103-114.
9. Cools H.J., Fraaije B.A., Kim S.H., and Lucas J.A. Impact of changes in the target P450 CYP51 enzyme associated with altered triazole-sensitivity in fungal pathogens of cereal crops. Biochemical Society Transactions 34 (2006) 1219-1222
10. Cools H.J., and Fraaije B.A. Evolution of azole resistance mechanisms in UK populations of Mycosphaerella graminicola. Fungicide resistance: are we winning the battle but loosing the war?. Aspects of Applied Biology 78 (2006) 21-27
11. Cools, H.J., Bean, T.P., Motteram, T.P., Gilbert, S.R., Fraaije, B.A., 2007. Molecular mechanisms associated with altered azole sensitivity in Mycosphaerella graminicola. In: BCPC International Congress, Crop Science and Technology, Pests and Disease, pp. 406-407.
12. Delye C., Laigret F., and Corio-Costet M.F. A mutation in the 14α-demethylase gene of Uncinula necator that correlates with resistance to a sterol biosynthesis inhibitor. Applied and Environmental Microbiology 63 (1997) 2966-2970
13. Fraaije B.A., Cools H.J., Kim S.H., Motteram J., Clark W.S., and Lucas J.A. A novel substitution I381V in the sterol 14α-demethylase (CYP51) of Mycosphaerella graminicola is differentially selected by azole fungicides. Molecular Plant Pathology 8 (2007) 245-254
14. Fraaije, B.A., Cools, H.J., Motteram, J., Gilbert, S., Kim, S.H., Lucas, J.A., 2008. Adaptation of Mycosphaerella graminicola populations to azole fungicides in the UK. In: Modern Fungicides and Antifungal Compounds V. pp. 121-127.
15. Fraaije B.A., and Cools H.J. Are azole fungicides losing ground against Septoria wheat disease? Resistance mechanisms in Mycosphaerella graminicola. Pest Management Science 64 (2008) 681-684
16. FRAC. Available from: (2008).
17. Leroux P., Walker A.S., Albertini A., and Gredt M. Resistance to fungicides in French populations of Septoria tritici, the causal agent of wheat leaf blotch. Fungicide resistance: are we winning the battle but loosing the war?. Aspects of Applied Biology 78 (2006) 153-162
18. Leroux P., Albertini A., Gautier A., Gredt M., and Walker A.S. Mutations in the CYP51 gene correlated with changes in sensitivity to sterol 14α-demethylation inhibitors in field isolates of Mycosphaerella graminicola. Pest Management Science 63 (2007) 688-698
19. Ma Z., Proffer T.J., Jacobs J.L., and Sundin G.W. Overexpression of the 14α-demethylase target gene (CYP51) mediates fungicide resistance in Blumeriella jaapii. Applied and Environmental Microbiology 72 (2006) 2581-2585
20. Morschhauser J. The genetic basis of fluconazole resistance development in Candida albicans. Biochimica et Biophysica Acta 1578 (2002) 240-248
21. Stammler G., Strobel D., Semar M., and Klappach K. Diagnostics of fungicide resistance and relevance of laboratory data for the field. Fungicide resistance: are we winning the battle but loosing the war?. Aspects of Applied Biology 78 (2006) 29-36
22. Stammler, G., Kern, L., Semar, M., Glaettli, A., Schoefl, U., 2008a. Sensitivity of Mycosphaerella graminicola to DMI fungicides related to mutations in the target gene cyp51 (14α-demethylase). In: Modern Fungicides and Antifungal Compounds V. pp. 137-142.
23. Stammler, G., Semar, M., Strobel, D., Schoefl, U., 2008b. Studies on potential factors affecting the control of Mycosphaerella graminicola in the field. Modern fungicides and Antifungal Compounds V. pp. 187-191.
24. Stergiopoulos I., Van Nistelroy J.G.M., Kema G.H.J., and De Waard M.A. Multiple mechanisms account for variation in base-line sensitivity to azole fungicides in field isolates of Mycosphaerella graminicola. Pest Management Science 59 (2003) 1333-1343
25. Wyand R.A., and Brown J.K. Sequence variation in the CYP51 gene of Blumeria graminis associated with resistance to sterol demethylase inhibiting fungicides. Fungal Genetics and Biology 42 (2005) 726-735
26. Zwiers L.H., Stergiopoulus I., Van Nistelroy J.G.M., and De Waard M.A. ABC transporters and azole sensitivity in laboratory strains of the wheat pathogen Mycosphaerella graminicola. Antimicrobial Agents and Chemotherapy 46 (2002) 3900-3906