Analysis of the CYP51 gene and encoded protein in propiconazole-resistant isolates of Mycosphaerella fijiensis

Pest Management Science

Volumn 65, Issue 8, 2009, Pages 892-899

  Cañas Gutiérrez, Gloria P ^a   Angarita Velásquez, Mónica J ^a   Restrepo Flórez, Juan M ^a   Rodríguez, Paola ^b   Moreno, Claudia X ^c   Arango, Rafael ^a,c  

^a Corporación para Investigaciones Biológicas UNALMED CIB ^*  (Colombia)

^b Centro de Investigaciones del Banano   (Colombia)

^c UNIVERSIDAD NACIONAL DE COLOMBIA   (Colombia)

Author keywords

Azole; Black sigatoka; DMI fungicides; Fungicide resistance; Mycosphaerella fijiensis; Propiconazole

Indexed keywords

CYTOCHROME P450; FUNGAL PROTEIN; PROPICONAZOLE; STEROL 14ALPHA DEMETHYLASE; TRIAZOLE DERIVATIVE;

DISEASE CONTROL; FUNGAL DISEASE; FUNGICIDE; FUNGUS; GENE EXPRESSION; GENETIC ANALYSIS; HOMOLOGY; INHIBITOR; PESTICIDE RESISTANCE; PROTEIN; QUANTITATIVE ANALYSIS;

AMINO ACID SEQUENCE; ANTIFUNGAL RESISTANCE; ARTICLE; ASCOMYCETES; CHEMICAL STRUCTURE; CHEMISTRY; ENZYME ACTIVE SITE; FUNGAL GENE; GENE EXPRESSION REGULATION; GENETICS; MOLECULAR GENETICS; MUTATION; PHYSIOLOGY; SEQUENCE HOMOLOGY;

AMINO ACID SEQUENCE; ASCOMYCOTA; CATALYTIC DOMAIN; CYTOCHROME P-450 ENZYME SYSTEM; DRUG RESISTANCE, FUNGAL; FUNGAL PROTEINS; GENE EXPRESSION REGULATION, FUNGAL; GENES, FUNGAL; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTATION; SEQUENCE HOMOLOGY; STEROL 14-DEMETHYLASE; TRIAZOLES;

FUNGI; MYCOBACTERIUM TUBERCULOSIS; MYCOSPHAERELLA FIJIENSIS;

EID: 69249221482     PISSN: 1526498X     EISSN: 15264998     Source Type: Journal    
DOI: 10.1002/ps.1770     Document Type: Article

References (35)

1. Cañas-Gutiérrez GP, Patiño LF, Rodríguez-Arango E and Arango R, Molecular characterization of benomyl-resistant isolates of Mycosphaerella fijiensis, collected in Colombia. J Phytopathol 154:403-409 (2006).
2. Fraaije BA, Cools HJ, Kim SH, Motteram J, Clark WS and Lucas JA, A novel substitution I381V in the sterol 14α-demethylase (CYP51) of Mycosphaerella graminicola is differentially selected by azole fungicides. Mol Plant Pathol 8:245-254 (2007).
3. Lepesheva G and Waterman M, CYP51 - the omnipotent P450. Mol Cell Endocrinol 215:165-170 (2004).
4. Lamb D, Kelly D and Kelly S, Molecular diversity of sterol 14α-demethylase substrates in plants, fungi and humans. FEBS Lett 425:263-265 (1998).
5. Ma Z and Michailides T, Advances in understanding molecular mechanisms of fungicide resistance and molecular detection of resistant genotypes in phytopathogenic fungi. Crop Prot 24:853-863 (2005).
6. Knight S, Wirz M, Amil A and Cook A, Fungicide resistance in Mycosphaerella fijiensis Morelet: current status and outlook. Proc 15th Acorbat Meeting, Cartagena de Indias, Colombia, pp. 163-166 (2002).
7. Romero RA and Sutton TB, Sensitivity of Mycosphaerella fijiensis, causal agent of Black Sigatoka of banana, to propiconazole. Phytopathology 87:96-100 (1996).
8. Balashov SV, Gardiner R, Park S and Perlin DS, Rapid, high-throughput, multiplex, real-time PCR for identification of mutations in the cyp51A gene of Aspergillus fumigatus that confer resistance to itraconazole. J Clin Microbiol 43:214-222 (2005).
9. Délye C, Laigret F and Corio-Costet M-F, A mutation in the 14α-demethylase gene of Uncinula necator that correlates with resistance to a sterol biosynthesis inhibitor. Appl Environ Microbiol 63:2966-2970 (1997).
10. Wyand R and Brown J, Sequence variations in the CYP51 gene of Blumeria graminis associated with resistance to sterol demethylase inhibiting fungicides. Fungal Genet Biol 42:726-735 (2005).
11. Ma Z, Proffer TJ, Jacobs JL and Sundin GW, Overexpressionofthe14α-demethylase target gene (CYP51) mediates fungicide resistance in Blumeriella jaapii. Appl Environ Microbiol 72:2581-2585 (2006).
12. Schnabel G and Jones AL, The 14α-demethylase (CYP51A1) gene is overexpressed in Venturia inequalis strains resistant to myclobutanil. Phytopathology 91:102-110 (2001).
13. Hayashi K, Schoonbeek HJ and De Waard MA, Expression of the ABC transporter BcatrD from Botrytis cinerea reduces sensitivity to sterol demethylation inhibitor fungicides. Pestic Biochem Physiol 73:110-121 (2002).
14. Zwiers LH, Stergiopoulos I, Van Nistelrooy JGM and De Waard MA, ABC transporters and azole susceptibility in laboratory strains of the wheat pathogen Mycosphaerella graminicola. Antimicrob Agents Chemother 46:3900-3906 (2002).
15. Podust L, Yermalitskaya LV, Lepesheva GI, Podust VN, Dalmasso EA and Waterman MR, Estriol bound and ligand-free structures of sterol 14alpha-demethylase. Structure 12:1937-1945 (2004).
16. Pelaez JE, Vásquez LE, Díaz TJ, Castañeda DA, Rodríguez E and Arango RE, Use of a micro-titre plate dilution assay to measure activity of antifungal compounds against Mycosphaerella fijiensis Morelet. Rev Fac Nal Agr Medellín 59:3425-3433(2006).
17. Cools H, Ishii JH, Butters JA and Hollomon DW, Cloning and sequence analysis of the eburicol 14α-demethylase encoding gene (CYP 51) from the Japanese pear scab fungus Venturia nashicola. J Phytopathol 150:444-450 (2002).
18. Huang SH, Inverse polymerase chain reaction. An efficient approach to cloning cDNA ends. Mol Biotechnol 2:15-22 (1994).
19. Siebert P, Chenchik DA, Kellog DE, Lukyanov KA and Lukyanov SA, An improved PCR method for walking in uncloned genomic DNA. Nucleic Acids Res 23:1087-1088 (1995).
20. Thompson JD, Higgins DG and Gibson TJ, CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673-4680 (1994).
21. Délye C, Laigret F and Corio-Costet M-F, Cloning and sequence analysis of the eburicol 14α-demethylase gene of the obligate biotrophic grape powdery mildew fungus. Gene 195:29-33 (1997).
22. Eswar N, Marti-Renom MA, Webb B, Madhusudhan MS, Eramian D, Shen M, et al, Comparative protein structure modeling with MODELLER, in Current Protocols in Bioinformatics, ed. By Baxevanis AD, Petsko GA, Stein LD and Stormo GD. John Wiley & Sons, Inc., Hoboken, NJ, Supplement 15, 5.6.1-5.6.30 (2006).
23. Fiser A, Do RK and Sali A, Modeling of loops in protein structures. Protein Sci 9:1753-1773 (2000).
24. Marti-Renom MA, Stuart A, Fiser A, Sánchez R, Melo F and Sali A, Comparative protein structure modeling of genes and genomes. Annu Rev Biophys Biomol Struct 29:291-325 (2000).
25. Sali A and Blundell TL, Comparative protein modeling by satisfaction of spatial restraints. J Mol Biol 234:779-815 (1993).
26. Gollapudy R, Ajmani S and Kulkarni S, Modelling and interactions of Aspergillus fumigatus lanosterol 14α-demethylase 'A' with azole antifungals. Bioorg Med Chem 12:2937-2950 (2004).
27. Xiao L, Madison V, Chau A, Loebenberg D, Palermo R and McNicholas P, Three dimensional models of wild type and mutated forms of cytochrome P450 14α-sterol demethylase from Aspergillus fumigatus and Candidaalbicans provide insights into posaconazole binding. Antimicrob Agents Chemother 48:568-574 (2004).
28. Guzman M, Current situation of Black Sigatoka disease in Costa Rica and options for the disease management. Proc 15th Acorbat Meeting, Cartagena de Indias, Colombia, pp. 184-192 (2002).
29. Manavathu EK, Kallakuri S, Arganoza MT and Vázquez JA, Amino acid variations of cytochrome P-450 lanosterol 14α-demethylase (CYP51A1) from fluconazole-resistant clinical isolates of Candida albicans. Rev Iberoam Micol 16:198-203 (1999).
30. Podust L, Poulos T and Waterman M, Crystal structure of cytochrome P450 14α-sterol demethylase (CYP51) from Mycobacterium tuberculosis in complex with azole inhibitors. Proc Natl Acad Sci USA 98:3068-3073 (2001).
31. Leroux P, Albertini C, Gautier A, Gredt M and Walker AS, Mutations in the CYP51 gene correlated with changes in sensitivity to sterol 14α-demethylation inhibitors in field isolates of Mycosphaerella graminicola. Pest Manag Sci 63:688-698 (2007).
32. Brunner PC, Stefanato FL and Mcdonald BA, Evolution of the CYP51 gene in Mycosphaerella graminicola: evidence for intragenic recombination and selective replacement. Mol Plant Pathol 9:305-316 (2007).
33. Albertson GD, Niimi M, Cannon RD and Jenkinson H, Multiple efflux mechanisms are involved in Candida albicans fluconazole resistance. Antimicrob Agents Chemother 40:2835-2841 (1996).
34. Sanglard D, Kuchler K, Ischer F, Pagani JL, Monod M and Bille J, Mechanisms of resistance to azole antifungal agents in Candida albicans isolates from AIDS patients involve specific multidrug transporters. Antimicrob Agents Chemother 39:2378-2386 (1995).
35. White TC, The presence of an R467K amino acid substitution and loss of allelic variation correlate with anazole-resistant lanosterol 14alpha-demethylase in Candida albicans. Antimicrob Agents Chemother 41:1488-1494 (1997).