Structural transitions within human Rad51 nucleoprotein filaments

Proceedings of the National Academy of Sciences of the United States of America

Volumn 106, Issue 31, 2009, Pages 12688-12693

  Robertson, Ragan B ^a   Moses, Dana N ^a   Kwon, YoungHo ^b   Chan, Pamela ^a   Chi, Peter ^b   Klein, Hannah ^c   Sung, Patrick ^b   Greene, Eric C ^a  

^a Och Spine at New York Presbyterian Hospitals   (United States)

^b YALE UNIVERSITY SCHOOL OF MEDICINE   (United States)

^c NEW YORK UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

DNA curtain; Homologous recombination; Single molecule imaging

Indexed keywords

ADENOSINE PHOSPHATE; ADENOSINE TRIPHOSPHATE; ADENYLYLIMIDODIPHOSPHATE; DNA; DOUBLE STRANDED DNA; NUCLEOPROTEIN; NUCLEOTIDE; OLIGOMER; RAD51 PROTEIN; CALCIUM; RAD51 PROTEIN, HUMAN; SODIUM CHLORIDE;

ARTICLE; COMPRESSION; CONFORMATIONAL TRANSITION; CONTROLLED STUDY; DISSOCIATION; DISSOCIATION CONSTANT; DOUBLE STRANDED DNA BREAK; HOMOLOGOUS RECOMBINATION; HUMAN; HYDROLYSIS; MICROSCOPY; MOLECULE; MUTANT; PRIORITY JOURNAL; PROTEIN ASSEMBLY; PROTEIN CONFORMATION; PROTEIN DNA BINDING; PROTEIN STRUCTURE; RNA TRANSLATION; STRUCTURE ANALYSIS; WILD TYPE; CHEMISTRY; FLUORESCENCE MICROSCOPY; PHYSIOLOGY;

EUKARYOTA;

ADENOSINE TRIPHOSPHATE; CALCIUM; DNA; HUMANS; HYDROLYSIS; MICROSCOPY, FLUORESCENCE; PROTEIN CONFORMATION; RAD51 RECOMBINASE; SODIUM CHLORIDE;

EID: 69149089856     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.0811465106     Document Type: Article

References (31)

1. Symington LS (2002) Role of RAD52 Epistasis group of genes in homologous recombination and double-strand break repair. Microbiol Mol Biol Rev 66:630-670.
2. San Filippo J, Sung P, Klein H (2008) Mechanism of eukaryotic homologous recombination. Annu Rev Biochem 77:229-257.
3. West SC (2003) Molecular views of recombination proteins and their control. Nat Rev 4:1-11.
4. Sung P, Krejci L, Van Komen S, Sehorn MG (2003) Rad51 recombinase and recombination mediators. J Biol Chem 278:42729-42732.
5. Baumann P, Benson FE, West SC (1996) Human Rad51 protein promotes ATP-dependent homologous pairing and strand transfer reactions in vitro. Cell 87:757-766.
6. Bianco PR, Tracy RB, Kowalczykowski SC (1998) DNA strand exchange proteins: A biochemical and physical comparison. Front Biosci 3:d530-603.
7. Eggler A, Inman R, Cox M (2002) Oct 18) The Rad51-dependent pairing of long DNA substrates is stabilized by replication protein A. J Biol Chem 277:39280-39288.
8. Lisby M, Rothstein R (2004 Jun) DNA damage checkpoint and repair centers. Curr Opin Cell Biol 16:328-334.
9. Symington LS, Heyer WD (2006) Some disassembly required: Role of DNA translocases in the disruption of recombination intermediates and dead-end complexes. Genes Dev 20:2479-2486.
10. Baumann P, West SC (1998) Role of the human Rad51 protein in homologous recombination and double-stranded-break repair. Trends Biochem Sci 23:247-251.
11. Yu X, Jacobs SA, West SC, Ogawa T, Egelman EH (2001) Domain structure and dynamics in the helical filaments formed by RecA and Rad51 on DNA. Proc Natl Acad Sci USA 98:8419-8424.
12. Sheridan SD, et al. (2008) A comparative analysis of Dmc1 and Rad51 nucleoprotein filaments. Nucleic Acids Res 36:4057-4066.
13. Kinebuchi T, et al. (2004) Structural basis for octomeric ring formation and DNA interaction of the human homologous-pairing protein Dmc1. Mol Cell 14:363-374.
14. Passy SI, Yu X, Li Z, Radding CM, Masson J-Y, West SC, Egelman EH (1999) Human Dmc1 protein binds DNA as an octomeric ring. Proc Natl Acad Sci USA 96:10684-10688.
15. Yu X, Egelman EH (1997) The RecA hexamer is a structural homologue of ring helicases. Nat Struct Biol 4:101-104.
16. Yang S, Yu X, Seitz EM, Kowalczykowski SC, Egelman EH (2001) Archaeal RadA protein binds DNA as both helical filaments and octameric rings. J Mol Biol 314:1077-1085.
17. Prasad TK, Yeykal CC, Greene EC (2006) Visualizing the assembly of human Rad51 filaments on double-stranded DNA. J Mol Biol 363:713-728.
18. Graneli A, Yeykal CC, Prasad TK, Greene EC (2006) Organized arrays of individual DNA molecules tethered to supported lipid bilayers. Langmuir 22:292-299.
19. Hilario J, Amitani I, Baskin RJ, Kowalczykowski SC (2009) Direct imaging of human Rad51 nucleoprotein dynamics on individual DNA molecules. Proc Natl Acad Sci USA 106:361-368.
20. Busygina V, Sehorn MG, Shi IY, Tsubouchi H, Roeder GS, Sung P (2008) Hed1 regulates Rad51-mediated recombination via a novel mechanism. Genes Dev 22:786-795.
21. 2+ activates human homologous recombination protein Rad51 by modulating its ATPase activity. Proc Natl Acad Sci USA 101:9988-9993.
22. Stark JM, Hu P, Pierce AJ, Moynahan ME, Ellis N, Jasin M (2002) ATP hydrolysis by mammalian Rad51 has a key role during homology-directed DNA repair. J Biol Chem 277:20185-20194.
23. Chi P, Van Komen S, Sehorn MG, Sigurdsson S, Sung P (2006) Roles of ATP binding and ATP hydrolysis in human Rad51 recombinase function. DNA Repair 5:381-391.
24. Nishinaka T, Doi Y, Hara R, Yashima E (2007) Elastic behavior of RecA-DNA helical filaments. J Mol Biol 370:837-845.
25. van Mameren J, Modesti M, Kanaar R, Wyman C, Peterman EJ, Wuite GJ (2009) Counting Rad51 proteins disassembling from nucleoprotein filaments under tension. Nature 457:745-748.
26. Galkin VE, et al. (2006) The Rad51/RadA N-terminal domain activates nucleoprotein filament ATPase activity. Structure 14:983-992.
27. Galkin VE, Esashi F, Yu X, Yang S, West SC, Egelman EH (2005) BRCA2 BRC motifs bind Rad51-DNA filaments. Proc Natl Acad Sci USA 102:8537-8542.
28. Esashi F, Galkin VE, Yu X, Egelman EH, West SC (2007) Stabilization of RAD51 nucleoprotein filaments by the C-terminal region of BRCA2. Nat Struct Mol Biol 14:468-474.
29. Petalcorin MI, Galkin VE, Yu X, Egelman EH, Boulton SJ (2007) Stabilization of RAD-51-DNA filaments via an interaction domain in Caenorhabditis elegans BRCA2. Proc Natl Acad Sci USA 104:8299-8304.
30. Jayathilaka K, et al. (2008) A chemical compound that stimulates the human homologous recombination protein RAD51. Proc Natl Acad Sci USA 105:15848-15853.
31. Prasad TK, Robertson RB, Visnapuu ML, Chi P, Sung P, Greene EC (2007) A DNA-translocating Snf2 molecular motor: Saccharomyces cerevisiae Rdh54 displays processive translocation and extrudes DNA loops. J Mol Biol 369:940-953.