메뉴 건너뛰기




Volumn 92, Issue 3, 2009, Pages 991-1001

The DNA integrity of cryopreserved spermatozoa separated for use in assisted reproductive technology is unaffected by the type of cryoprotectant used but is related to the DNA integrity of the fresh separated preparation

Author keywords

Cryopreservation; cryoprotectants; density gradient centrifugation; DNA fragmentation; reproductive technology; spermatozoa; TUNEL

Indexed keywords

CELL DNA; DNA FRAGMENT; PRESERVATION SOLUTION;

EID: 69049093082     PISSN: 00150282     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.fertnstert.2008.07.1747     Document Type: Article
Times cited : (18)

References (54)
  • 1
    • 0015624492 scopus 로고
    • Synopsis of the use of frozen human semen since 1964: state of the art of human semen banking
    • Sherman J.K. Synopsis of the use of frozen human semen since 1964: state of the art of human semen banking. Fertil Steril 24 (1973) 397-412
    • (1973) Fertil Steril , vol.24 , pp. 397-412
    • Sherman, J.K.1
  • 3
    • 5144230414 scopus 로고    scopus 로고
    • Cryopreservation of human spermatozoa: comparison of two cryopreservation methods and three cryoprotectants
    • Nallella K.P., Sharma R.K., Allamaneni S.S., Aziz N., and Agarwal A. Cryopreservation of human spermatozoa: comparison of two cryopreservation methods and three cryoprotectants. Fertil Steril 82 (2004) 913-918
    • (2004) Fertil Steril , vol.82 , pp. 913-918
    • Nallella, K.P.1    Sharma, R.K.2    Allamaneni, S.S.3    Aziz, N.4    Agarwal, A.5
  • 4
    • 0024337024 scopus 로고
    • Role of glutathione peroxidase in protecting mammalian spermatozoa from loss of motility caused by spontaneous lipid peroxidation
    • Alvarez J.G., and Storey B.T. Role of glutathione peroxidase in protecting mammalian spermatozoa from loss of motility caused by spontaneous lipid peroxidation. Gamete Res 23 (1989) 77-90
    • (1989) Gamete Res , vol.23 , pp. 77-90
    • Alvarez, J.G.1    Storey, B.T.2
  • 5
    • 34249783130 scopus 로고    scopus 로고
    • Impact of cryopreservation and reactive oxygen species on DNA integrity, lipid peroxidation, and functional parameters in ram sperm
    • Peris S.I., Bilodeau J.-F., Dufour M., and Bailey J.L. Impact of cryopreservation and reactive oxygen species on DNA integrity, lipid peroxidation, and functional parameters in ram sperm. Mol Reprod Dev 74 (2007) 878-892
    • (2007) Mol Reprod Dev , vol.74 , pp. 878-892
    • Peris, S.I.1    Bilodeau, J.-F.2    Dufour, M.3    Bailey, J.L.4
  • 6
    • 0034969575 scopus 로고    scopus 로고
    • Assessment of DNA integrity and morphology of ejaculated spermatozoa from fertile and infertile men before and after cryopreservation
    • Donnelly E.T., Steele E.K., McClure N., and Lewis S.E. Assessment of DNA integrity and morphology of ejaculated spermatozoa from fertile and infertile men before and after cryopreservation. Hum Reprod 16 (2001) 1191-1199
    • (2001) Hum Reprod , vol.16 , pp. 1191-1199
    • Donnelly, E.T.1    Steele, E.K.2    McClure, N.3    Lewis, S.E.4
  • 7
    • 0028242903 scopus 로고
    • Mechanism of superoxide dismutase loss from human sperm cells during cryopreservation
    • Lasso J.L., Noiles E.E., Alvarez J.G., and Storey B.T. Mechanism of superoxide dismutase loss from human sperm cells during cryopreservation. J Androl 15 (1994) 255-265
    • (1994) J Androl , vol.15 , pp. 255-265
    • Lasso, J.L.1    Noiles, E.E.2    Alvarez, J.G.3    Storey, B.T.4
  • 8
    • 0028320976 scopus 로고
    • A free radical theory of male infertility
    • discussion 24
    • Aitken R.J. A free radical theory of male infertility. Reprod Fertil Dev 6 (1994) 19-23 discussion 24
    • (1994) Reprod Fertil Dev , vol.6 , pp. 19-23
    • Aitken, R.J.1
  • 10
    • 1842532313 scopus 로고    scopus 로고
    • DNA integrity and motility of human spermatozoa after standard slow freezing versus cryoprotectant-free vitrification
    • Isachenko E., Isachenko V., Katkov II, Rahimi G., Schondorf T., Mallmann P., et al. DNA integrity and motility of human spermatozoa after standard slow freezing versus cryoprotectant-free vitrification. Hum Reprod 19 (2004) 932-939
    • (2004) Hum Reprod , vol.19 , pp. 932-939
    • Isachenko, E.1    Isachenko, V.2    Katkov II3    Rahimi, G.4    Schondorf, T.5    Mallmann, P.6
  • 11
    • 27944478245 scopus 로고    scopus 로고
    • DNA damage to spermatozoa has impacts on fertilization and pregnancy
    • Lewis S.E., and Aitken R.J. DNA damage to spermatozoa has impacts on fertilization and pregnancy. Cell Tissue Res 322 (2005) 33-41
    • (2005) Cell Tissue Res , vol.322 , pp. 33-41
    • Lewis, S.E.1    Aitken, R.J.2
  • 12
    • 0038060337 scopus 로고    scopus 로고
    • Sperm DNA fragmentation decreases the pregnancy rate in an assisted reproductive technique
    • Benchaib M., Braun V., Lornage J., Hadj S., Salle B., Lejeune H., et al. Sperm DNA fragmentation decreases the pregnancy rate in an assisted reproductive technique. Hum Reprod 18 (2003) 1023-1028
    • (2003) Hum Reprod , vol.18 , pp. 1023-1028
    • Benchaib, M.1    Braun, V.2    Lornage, J.3    Hadj, S.4    Salle, B.5    Lejeune, H.6
  • 13
    • 4143065747 scopus 로고    scopus 로고
    • Extent of nuclear DNA damage in ejaculated spermatozoa impacts on blastocyst development after in vitro fertilization
    • Seli E., Gardner D.K., Schoolcraft W.B., Moffatt O., and Sakkas D. Extent of nuclear DNA damage in ejaculated spermatozoa impacts on blastocyst development after in vitro fertilization. Fertil Steril 82 (2004) 378-383
    • (2004) Fertil Steril , vol.82 , pp. 378-383
    • Seli, E.1    Gardner, D.K.2    Schoolcraft, W.B.3    Moffatt, O.4    Sakkas, D.5
  • 14
    • 33845726528 scopus 로고    scopus 로고
    • Sperm deoxyribonucleic acid fragmentation as a prognostic indicator of assisted reproductive technology outcome
    • Benchaib M., Lornage J., Mazoyer C., Lejeune H., Salle B., and Francois Guerin J. Sperm deoxyribonucleic acid fragmentation as a prognostic indicator of assisted reproductive technology outcome. Fertil Steril 87 (2007) 93-100
    • (2007) Fertil Steril , vol.87 , pp. 93-100
    • Benchaib, M.1    Lornage, J.2    Mazoyer, C.3    Lejeune, H.4    Salle, B.5    Francois Guerin, J.6
  • 15
    • 33749993552 scopus 로고    scopus 로고
    • Sperm DNA fragmentation: paternal effect on early post-implantation embryo development in ART
    • Borini A., Tarozzi N., Bizzaro D., Bonu M.A., Fava L., Flamigni C., et al. Sperm DNA fragmentation: paternal effect on early post-implantation embryo development in ART. Hum Reprod 21 (2006) 2876-2881
    • (2006) Hum Reprod , vol.21 , pp. 2876-2881
    • Borini, A.1    Tarozzi, N.2    Bizzaro, D.3    Bonu, M.A.4    Fava, L.5    Flamigni, C.6
  • 16
    • 0036906176 scopus 로고    scopus 로고
    • Sperm DNA quality predicts intrauterine insemination outcome: a prospective cohort study
    • Duran E.H., Morshedi M., Taylor S., and Oehninger S. Sperm DNA quality predicts intrauterine insemination outcome: a prospective cohort study. Hum Reprod 17 (2002) 3122-3128
    • (2002) Hum Reprod , vol.17 , pp. 3122-3128
    • Duran, E.H.1    Morshedi, M.2    Taylor, S.3    Oehninger, S.4
  • 18
    • 0031039736 scopus 로고    scopus 로고
    • Determination of optimal cryoprotectants and procedures for their addition and removal from human spermatozoa
    • Gilmore J.A., Liu J., Gao D.Y., and Critser J.K. Determination of optimal cryoprotectants and procedures for their addition and removal from human spermatozoa. Hum Reprod 12 (1997) 112-118
    • (1997) Hum Reprod , vol.12 , pp. 112-118
    • Gilmore, J.A.1    Liu, J.2    Gao, D.Y.3    Critser, J.K.4
  • 19
    • 0031668481 scopus 로고    scopus 로고
    • Comparison of various methods of processing human cryopreserved-thawed semen samples
    • Srisombut C., Morshedi M., Lin M.H., Nassar A., and Oehninger S. Comparison of various methods of processing human cryopreserved-thawed semen samples. Hum Reprod 13 (1998) 2151-2157
    • (1998) Hum Reprod , vol.13 , pp. 2151-2157
    • Srisombut, C.1    Morshedi, M.2    Lin, M.H.3    Nassar, A.4    Oehninger, S.5
  • 20
    • 0033967301 scopus 로고    scopus 로고
    • Cryoprotective agent and temperature effects on human sperm membrane permeabilities: convergence of theoretical and empirical approaches for optimal cryopreservation methods
    • Gilmore J.A., Liu J., Woods E.J., Peter A.T., and Critser J.K. Cryoprotective agent and temperature effects on human sperm membrane permeabilities: convergence of theoretical and empirical approaches for optimal cryopreservation methods. Hum Reprod 15 (2000) 335-343
    • (2000) Hum Reprod , vol.15 , pp. 335-343
    • Gilmore, J.A.1    Liu, J.2    Woods, E.J.3    Peter, A.T.4    Critser, J.K.5
  • 22
    • 22244472706 scopus 로고    scopus 로고
    • Sperm DNA fragmentation negatively correlates with velocity and fertilization rates but might not affect pregnancy rates
    • Huang C.-C., Lin D.P.-C., Tsao H.-M., Cheng T.-C., Liu C.-H., and Lee M.-S. Sperm DNA fragmentation negatively correlates with velocity and fertilization rates but might not affect pregnancy rates. Fertil Steril 84 (2005) 130-140
    • (2005) Fertil Steril , vol.84 , pp. 130-140
    • Huang, C.-C.1    Lin, D.P.-C.2    Tsao, H.-M.3    Cheng, T.-C.4    Liu, C.-H.5    Lee, M.-S.6
  • 23
    • 26644464402 scopus 로고    scopus 로고
    • On the relation between initial value and slope
    • Byth K., and Cox D.R. On the relation between initial value and slope. Biostatistics 6 (2005) 395-403
    • (2005) Biostatistics , vol.6 , pp. 395-403
    • Byth, K.1    Cox, D.R.2
  • 24
    • 0033027767 scopus 로고    scopus 로고
    • Effect of freeze-thawing procedure on chromatin stability, morphological alteration and membrane integrity of human spermatozoa in fertile and subfertile men
    • Hammadeh M.E., Askari A.S., Georg T., Rosenbaum P., and Schmidt W. Effect of freeze-thawing procedure on chromatin stability, morphological alteration and membrane integrity of human spermatozoa in fertile and subfertile men. Int J Androl 22 (1999) 155-162
    • (1999) Int J Androl , vol.22 , pp. 155-162
    • Hammadeh, M.E.1    Askari, A.S.2    Georg, T.3    Rosenbaum, P.4    Schmidt, W.5
  • 25
    • 0032866801 scopus 로고    scopus 로고
    • In vitro sperm-binding assay to distinguish differences in populations of human sperm or damage to sperm resulting from cryopreservation
    • Amann R.P., Shabanowitz R.B., Huszar G., and Broder S.J. In vitro sperm-binding assay to distinguish differences in populations of human sperm or damage to sperm resulting from cryopreservation. J Androl 20 (1999) 648-654
    • (1999) J Androl , vol.20 , pp. 648-654
    • Amann, R.P.1    Shabanowitz, R.B.2    Huszar, G.3    Broder, S.J.4
  • 26
    • 33748103006 scopus 로고    scopus 로고
    • Effect of cryopreservation on sperm apoptotic deoxyribonucleic acid fragmentation in patients with oligozoospermia
    • de Paula T.S., Bertolla R.P., Spaine D.M., Cunha M.A., Schor N., and Cedenho A.P. Effect of cryopreservation on sperm apoptotic deoxyribonucleic acid fragmentation in patients with oligozoospermia. Fertil Steril 86 (2006) 597-600
    • (2006) Fertil Steril , vol.86 , pp. 597-600
    • de Paula, T.S.1    Bertolla, R.P.2    Spaine, D.M.3    Cunha, M.A.4    Schor, N.5    Cedenho, A.P.6
  • 27
    • 0034941859 scopus 로고    scopus 로고
    • Cryopreservation-thawing of fractionated human spermatozoa is associated with membrane phosphatidylserine externalization and not DNA fragmentation
    • Duru N.K., Morshedi M.S., Schuffner A., and Oehninger S. Cryopreservation-thawing of fractionated human spermatozoa is associated with membrane phosphatidylserine externalization and not DNA fragmentation. J Androl 22 (2001) 646-651
    • (2001) J Androl , vol.22 , pp. 646-651
    • Duru, N.K.1    Morshedi, M.S.2    Schuffner, A.3    Oehninger, S.4
  • 28
    • 9744233682 scopus 로고    scopus 로고
    • Cryopreservation and thawing is associated with varying extent of activation of apoptotic machinery in subsets of ejaculated human spermatozoa
    • Paasch U., Sharma R.K., Gupta A.K., Grunewald S., Mascha E.J., Thomas Jr. A.J., et al. Cryopreservation and thawing is associated with varying extent of activation of apoptotic machinery in subsets of ejaculated human spermatozoa. Biol Reprod 71 (2004) 1828-1837
    • (2004) Biol Reprod , vol.71 , pp. 1828-1837
    • Paasch, U.1    Sharma, R.K.2    Gupta, A.K.3    Grunewald, S.4    Mascha, E.J.5    Thomas Jr., A.J.6
  • 29
    • 0031045420 scopus 로고    scopus 로고
    • Detection of deoxyribonucleic acid fragmentation in human sperm: correlation with fertilization in vitro
    • Sun J.G., Jurisicova A., and Casper R.F. Detection of deoxyribonucleic acid fragmentation in human sperm: correlation with fertilization in vitro. Biol Reprod 56 (1997) 602-607
    • (1997) Biol Reprod , vol.56 , pp. 602-607
    • Sun, J.G.1    Jurisicova, A.2    Casper, R.F.3
  • 30
    • 0031895751 scopus 로고    scopus 로고
    • Sperm deoxyribonucleic acid fragmentation is increased in poor-quality semen samples and correlates with failed fertilization in intracytoplasmic sperm injection
    • Lopes S., Sun J.G., Jurisicova A., Meriano J., and Casper R.F. Sperm deoxyribonucleic acid fragmentation is increased in poor-quality semen samples and correlates with failed fertilization in intracytoplasmic sperm injection. Fertil Steril 69 (1998) 528-532
    • (1998) Fertil Steril , vol.69 , pp. 528-532
    • Lopes, S.1    Sun, J.G.2    Jurisicova, A.3    Meriano, J.4    Casper, R.F.5
  • 31
    • 0029116225 scopus 로고
    • Total antioxidant capacity of seminal plasma is different in fertile and infertile men
    • Lewis S.E., Boyle P.M., McKinney K.A., Young I.S., and Thompson W. Total antioxidant capacity of seminal plasma is different in fertile and infertile men. Fertil Steril 64 (1995) 868-870
    • (1995) Fertil Steril , vol.64 , pp. 868-870
    • Lewis, S.E.1    Boyle, P.M.2    McKinney, K.A.3    Young, I.S.4    Thompson, W.5
  • 32
    • 1842585237 scopus 로고    scopus 로고
    • Structure of human sperm DNA and background damage, analysed by in situ enzymatic treatment and digital image analysis
    • Muriel L., Segrelles E., Goyanes V., Gosalvez J., and Fernandez J.L. Structure of human sperm DNA and background damage, analysed by in situ enzymatic treatment and digital image analysis. Mol Hum Reprod 10 (2004) 203-209
    • (2004) Mol Hum Reprod , vol.10 , pp. 203-209
    • Muriel, L.1    Segrelles, E.2    Goyanes, V.3    Gosalvez, J.4    Fernandez, J.L.5
  • 33
    • 0021202164 scopus 로고
    • Freezing of living cells: mechanisms and implications
    • Mazur P. Freezing of living cells: mechanisms and implications. Am J Physiol 247 (1984) C125-C142
    • (1984) Am J Physiol , vol.247
    • Mazur, P.1
  • 34
    • 0027204801 scopus 로고
    • Hyperosmotic tolerance of human spermatozoa: separate effects of glycerol, sodium chloride, and sucrose on spermolysis
    • Gao D.Y., Ashworth E., Watson P.F., Kleinhans F.W., Mazur P., and Critser J.K. Hyperosmotic tolerance of human spermatozoa: separate effects of glycerol, sodium chloride, and sucrose on spermolysis. Biol Reprod 49 (1993) 112-123
    • (1993) Biol Reprod , vol.49 , pp. 112-123
    • Gao, D.Y.1    Ashworth, E.2    Watson, P.F.3    Kleinhans, F.W.4    Mazur, P.5    Critser, J.K.6
  • 35
    • 0025091810 scopus 로고
    • Cryopreservation of mammalian sperm: what we ask them to survive
    • Hammerstedt R.H., Graham J.K., and Nolan J.P. Cryopreservation of mammalian sperm: what we ask them to survive. J Androl 11 (1990) 73-88
    • (1990) J Androl , vol.11 , pp. 73-88
    • Hammerstedt, R.H.1    Graham, J.K.2    Nolan, J.P.3
  • 36
    • 0027237261 scopus 로고
    • Evidence that membrane stress contributes more than lipid peroxidation to sublethal cryodamage in cryopreserved human sperm: glycerol and other polyols as sole cryoprotectant
    • Alvarez J.G., and Storey B.T. Evidence that membrane stress contributes more than lipid peroxidation to sublethal cryodamage in cryopreserved human sperm: glycerol and other polyols as sole cryoprotectant. J Androl 14 (1993) 199-209
    • (1993) J Androl , vol.14 , pp. 199-209
    • Alvarez, J.G.1    Storey, B.T.2
  • 37
    • 0028891932 scopus 로고
    • Differential incorporation of fatty acids into and peroxidative loss of fatty acids from phospholipids of human spermatozoa
    • Alvarez J.G., and Storey B.T. Differential incorporation of fatty acids into and peroxidative loss of fatty acids from phospholipids of human spermatozoa. Mol Reprod Dev 42 (1995) 334-346
    • (1995) Mol Reprod Dev , vol.42 , pp. 334-346
    • Alvarez, J.G.1    Storey, B.T.2
  • 38
    • 0031805926 scopus 로고    scopus 로고
    • Analysis of the impact of intracellular reactive oxygen species generation on the structural and functional integrity of human spermatozoa: lipid peroxidation, DNA fragmentation and effectiveness of antioxidants
    • Twigg J., Fulton N., Gomez E., Irvine D.S., and Aitken R.J. Analysis of the impact of intracellular reactive oxygen species generation on the structural and functional integrity of human spermatozoa: lipid peroxidation, DNA fragmentation and effectiveness of antioxidants. Hum Reprod 13 (1998) 1429-1436
    • (1998) Hum Reprod , vol.13 , pp. 1429-1436
    • Twigg, J.1    Fulton, N.2    Gomez, E.3    Irvine, D.S.4    Aitken, R.J.5
  • 41
    • 44349169930 scopus 로고    scopus 로고
    • Effect of cryopreservation on sperm DNA integrity in patients with teratospermia
    • Kalthur G., Adiga S.K., Upadhya D., Rao S., and Kumar P. Effect of cryopreservation on sperm DNA integrity in patients with teratospermia. Fertil Steril 89 (2008) 1723-1727
    • (2008) Fertil Steril , vol.89 , pp. 1723-1727
    • Kalthur, G.1    Adiga, S.K.2    Upadhya, D.3    Rao, S.4    Kumar, P.5
  • 42
    • 11144228328 scopus 로고    scopus 로고
    • Sperm DNA integrity: correlation with sperm plasma membrane integrity in semen evaluated for male infertility
    • Moskovtsev S.I., Willis J., Azad A., and Mullen J.B.M. Sperm DNA integrity: correlation with sperm plasma membrane integrity in semen evaluated for male infertility. Arch Androl 51 (2005) 33-40
    • (2005) Arch Androl , vol.51 , pp. 33-40
    • Moskovtsev, S.I.1    Willis, J.2    Azad, A.3    Mullen, J.B.M.4
  • 43
    • 0021336185 scopus 로고
    • Development of an assay to assess the functional integrity of the human sperm membrane and its relationship to other semen characteristics
    • Jeyendran R.S., Van der Ven H.H., Perez-Pelaez M., Crabo B.G., and Zaneveld L.J. Development of an assay to assess the functional integrity of the human sperm membrane and its relationship to other semen characteristics. J Reprod Fertil 70 (1984) 219-228
    • (1984) J Reprod Fertil , vol.70 , pp. 219-228
    • Jeyendran, R.S.1    Van der Ven, H.H.2    Perez-Pelaez, M.3    Crabo, B.G.4    Zaneveld, L.J.5
  • 44
    • 0033776213 scopus 로고    scopus 로고
    • Membrane fluidity predicts the outcome of cryopreservation of human spermatozoa
    • Giraud M.N., Motta C., Boucher D., and Grizard G. Membrane fluidity predicts the outcome of cryopreservation of human spermatozoa. Hum Reprod 15 (2000) 2160-2164
    • (2000) Hum Reprod , vol.15 , pp. 2160-2164
    • Giraud, M.N.1    Motta, C.2    Boucher, D.3    Grizard, G.4
  • 46
    • 19944433572 scopus 로고    scopus 로고
    • Efficient treatment of infertility due to sperm DNA damage by ICSI with testicular spermatozoa
    • Greco E., Scarselli F., Iacobelli M., Rienzi L., Ubaldi F., Ferrero S., et al. Efficient treatment of infertility due to sperm DNA damage by ICSI with testicular spermatozoa. Hum Reprod 20 (2005) 226-230
    • (2005) Hum Reprod , vol.20 , pp. 226-230
    • Greco, E.1    Scarselli, F.2    Iacobelli, M.3    Rienzi, L.4    Ubaldi, F.5    Ferrero, S.6
  • 47
    • 18344365004 scopus 로고    scopus 로고
    • Sperm count and chromatin structure in men exposed to inorganic lead: lowest adverse effect levels
    • Bonde J.P., Joffe M., Apostoli P., Dale A., Kiss P., Spano M., et al. Sperm count and chromatin structure in men exposed to inorganic lead: lowest adverse effect levels. Occup Environ Med 59 (2002) 234-242
    • (2002) Occup Environ Med , vol.59 , pp. 234-242
    • Bonde, J.P.1    Joffe, M.2    Apostoli, P.3    Dale, A.4    Kiss, P.5    Spano, M.6
  • 48
    • 0031683878 scopus 로고    scopus 로고
    • The applicability of the flow cytometric sperm chromatin structure assay in epidemiological studies
    • Spano M., Kolstad A.H., Larsen S.B., Cordelli E., Leter G., Giwercman A., et al. The applicability of the flow cytometric sperm chromatin structure assay in epidemiological studies. Hum Reprod 13 (1998) 2495-2505
    • (1998) Hum Reprod , vol.13 , pp. 2495-2505
    • Spano, M.1    Kolstad, A.H.2    Larsen, S.B.3    Cordelli, E.4    Leter, G.5    Giwercman, A.6
  • 49
    • 1842833133 scopus 로고    scopus 로고
    • The impact of testicular and accessory sex gland function on sperm chromatin integrity as assessed by the sperm chromatin structure assay (SCSA)
    • Richthoff J., Spano M., Giwercman Y.L., Frohm B., Jepson K., Malm J., et al. The impact of testicular and accessory sex gland function on sperm chromatin integrity as assessed by the sperm chromatin structure assay (SCSA). Hum Reprod 17 (2002) 3162-3169
    • (2002) Hum Reprod , vol.17 , pp. 3162-3169
    • Richthoff, J.1    Spano, M.2    Giwercman, Y.L.3    Frohm, B.4    Jepson, K.5    Malm, J.6
  • 50
    • 0026366134 scopus 로고
    • Individuality of DNA denaturation patterns in human sperm as measured by the sperm chromatin structure assay
    • Evenson D.P., Jost L.K., Baer R.K., Turner T.W., and Schrader S.M. Individuality of DNA denaturation patterns in human sperm as measured by the sperm chromatin structure assay. Reprod Toxicol 5 (1991) 115-125
    • (1991) Reprod Toxicol , vol.5 , pp. 115-125
    • Evenson, D.P.1    Jost, L.K.2    Baer, R.K.3    Turner, T.W.4    Schrader, S.M.5
  • 52
    • 21644458568 scopus 로고    scopus 로고
    • Longitudinal study of sperm DNA fragmentation as measured by terminal uridine nick end-labelling assay
    • Sergerie M., Laforest G., Boulanger K., Bissonnette F., and Bleau G. Longitudinal study of sperm DNA fragmentation as measured by terminal uridine nick end-labelling assay. Hum Reprod 20 (2005) 1921-1927
    • (2005) Hum Reprod , vol.20 , pp. 1921-1927
    • Sergerie, M.1    Laforest, G.2    Boulanger, K.3    Bissonnette, F.4    Bleau, G.5
  • 53
    • 0028097272 scopus 로고
    • The status and the state of the human epididymis
    • Bedford J.M. The status and the state of the human epididymis. Hum Reprod 9 (1994) 2187-2199
    • (1994) Hum Reprod , vol.9 , pp. 2187-2199
    • Bedford, J.M.1
  • 54
    • 0034814823 scopus 로고    scopus 로고
    • Characterization of subsets of human spermatozoa at different stages of maturation: implications in the diagnosis and treatment of male infertility
    • Ollero M., Gil-Guzman E., Lopez M.C., Sharma R.K., Agarwal A., Larson K., et al. Characterization of subsets of human spermatozoa at different stages of maturation: implications in the diagnosis and treatment of male infertility. Hum Reprod 16 (2001) 1912-1921
    • (2001) Hum Reprod , vol.16 , pp. 1912-1921
    • Ollero, M.1    Gil-Guzman, E.2    Lopez, M.C.3    Sharma, R.K.4    Agarwal, A.5    Larson, K.6


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.