The partial substrate dethiaacetyl-coenzyme A mimics all critical carbon acid reactions in the condensation half-reaction catalyzed by Thermoplasma acidophilum citrate synthase

Biochemistry

Volumn 48, Issue 33, 2009, Pages 7878-7891

  Kurz, Linda C ^a   Constantine, Charles Z ^b   Jiang, Hong ^b   Kappock, T Joseph ^c  

^a WASHINGTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b WASHINGTON UNIVERSITY   (United States)

^c PURDUE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

[CARBONYL; ACETYL-COA; ACTIVE SITE RESIDUES; CARBON ACID; CITRATE SYNTHASE; COENZYME A; ENOLATE INTERMEDIATES; HALF-REACTIONS; ISOTOPE EFFECT; METHYL CARBON; REACTION CATALYZED; STABLE COMPLEXES; STEADY-STATE FLUORESCENCE; STEADY-STATE KINETICS; STOPPED FLOW; THIOESTERS;

CONDENSATION; ENZYMES; ISOTOPES; KETONES; SUBSTRATES;

CONDENSATION REACTIONS;

ACETYL COENZYME A; ASPARTIC ACID; CARBOXYAMIDOMETHYL COENZYME A; CITRATE SYNTHASE; COENZYME A; DETHIACITRYL COENZYME; DIETHIAACETYL COENZYME A; OXALOACETIC ACID; UNCLASSIFIED DRUG;

ARTICLE; CATALYSIS; COMPLEX FORMATION; CONTROLLED STUDY; DISSOCIATION CONSTANT; ENZYME KINETICS; ENZYME SUBSTRATE; FLUORESCENCE ANALYSIS; PH; POLYMERIZATION; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS; STEADY STATE; THERMOPLASMA ACIDOPHILUM;

ACETYL COENZYME A; ANIMALS; ASPARTIC ACID; CATALYSIS; CATALYTIC DOMAIN; CITRATE (SI)-SYNTHASE; CRYSTALLOGRAPHY, X-RAY; ESTERS; HYDROGEN-ION CONCENTRATION; HYDROLYSIS; KETONES; KINETICS; MOLECULAR MIMICRY; SUBSTRATE SPECIFICITY; SWINE; THERMOPLASMA; TRYPTOPHAN;

SUIDAE; THERMOPLASMA ACIDOPHILUM;

EID: 69049092900     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi9006447     Document Type: Article

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