Use of the RAPD-PCR fingerprinting and API system for clustering lactic acid bacteria isolated from traditional Sudanese sour milk (Roab)

African Journal of Biotechnology

Volumn 8, Issue 15, 2009, Pages 3399-3404

  Hamza, Abdelhalim A ^a   El Gaali, Eisa I ^a   Mahdi, Ahmed A ^b  

^a National Center for Research Sudan   (Sudan)

^b UNIVERSITY OF KHARTOUM   (Sudan)

Author keywords

Lactic acid; Lactobacillus; Molecular markers; RAPD PCR

Indexed keywords

EXOPOLYSACCHARIDE; SUCROSE;

ARTICLE; BACTERIAL STRAIN; BACTERIUM COLONY; FINGER DERMATOGLYPHICS; GENE CLUSTER; GENETIC VARIABILITY; ISOLATION PROCEDURE; LACTIC ACID BACTERIUM; MILK; NONHUMAN; POLYMERASE CHAIN REACTION; RANDOM AMPLIFIED POLYMORPHIC DNA;

AEROCOCCUS; ENTEROCOCCUS FAECIUM; ENTEROCOCCUS GALLINARUM; LACTOBACILLUS; LACTOBACILLUS CASEI; LACTOBACILLUS PENTOSUS; LACTOBACILLUS PLANTARUM; LACTOBACILLUS RHAMNOSUS; LACTOCOCCUS LACTIS; LEUCONOSTOC; STREPTOCOCCUS ACIDOMINIMUS; STREPTOCOCCUS BOVIS;

EID: 68949098166     PISSN: None     EISSN: 16845315     Source Type: Journal    
DOI: None     Document Type: Article

References (26)

1. Abdelgadir WS, Hamad SH, Moler PL, Jakobsen M (2001). Characterization of the dominant microbiota of Sudanese fermented milk Rob. Int. Dairy J. 11: 63-70.
2. Carozzi NB, Kramer VC, Warren GW, Evola S, Koriel MG (1991). Prediction of insecticidal activity of Bacillus thuringiensis strain by polymerase chain reaction product profiles. J. Appl. Environ. Microbiol. 57: 3057-3061.
3. Cocconcelli PS, Parisi MG, Senini L, Bottazzi V (1997). Use of RAPD and 16S rDNA sequencing for the study of Lactobacillus population dynamics in natural whey culture. Lett. Appl. Microbiol. 25: 8-12.
4. Corsetti A, Gobetti M, Rossi J, Damiani P (1998). Antimould activity of sourdough lactic acid bacteria: Identification of a mixture of organic acids produced by Lactobacillus Sanfrancisco CB1. Appl. Microbiol. Biotechnol. 50: 253-256.
5. Cox RP Thomsen JK (1990). Computer-aided identification of lactic acid bacteria using the API 50 CHL system. Lett. Appl. Microbiol. 10: 257-259.
6. Doliezil L, Kirsop BH (1977). The use of the API Lactobacillus system for the identification of pedicocci. J. Appl. Bacteriol. 42: 213-217.
7. Hayford AE, Petersen A, Vogensen FK, Jakobsen M (1999). Use of conserved randomly amplified polymorphic DNA (RAPD) fragments for characterization of Lactobacillus fermentum in Ghanaian fermented maize dough. J. Appl. Environ. Microbiol. 65: 3213-3221.
8. Hitchener BJ, Egan AF, Rogers PJ (1982). Characterization of lactic acid bacteria isolated from vacuum-packaged beef. J. Appl. Bacteriol. 52: 31-37.
9. Jorgen J, Bruno P, Henrik C, Gulam R, John E, Bee W, Kharidam M (1999). Identification of Lactic Acid Bacteria from Chili Bo, a Malaysian Food Ingredient. J. Appl. Environ. Microbiol. 65: 599-605.
10. Garciela F (2001). Maintenance of lactic acidbacteria. In: Methods in: Biotechnology vol14: Food microbiology protocols. John F and Alicia L (eds). pp. 163-171. Humana Press Inc.
11. Gobbetti M, Lanciotti R, De Angelis M, Corbo M, Massini R, Fox F (1999). Study of the effects of temperature, pH and NaCl on the peptidase activities of non-starter lactic acid bacteria (NSLAB) by quadratic response surface methodology. Int. Dairy J. 9: 865-875.
12. Kebede A (2007). Isolation, characterization and identification of lactic acid bacteria involved in traditional fermentation of borde, an Ethiopian cereal beverage. Afr. J. Biotechnol. 6: 1469-1478
13. Klijn N, Weerkaamp A, De Vos WM (1991). Identification of mesophilic lactic acid bacteria by using polymerase chain reaction-amplified variable regions of 16S rRNA and specific DNA probes. J. Appl. Environ. Microbiol. 57: 3390-3393.
14. Le-Bourgeois P, Lautier M, Ritzenthaler P (1993). Chromosomal mapping in lactic acid bacteria. FEMS Microbiol. Rev. 12: 109-124.
15. Leisner J, Vancanneyt M, Goris J, Christensen H, Rusul G (2000). Description of Paralactobacillus selangorensis gen. nov., sp. nov., a new lactic acid bacterium isolated from chili bo, a Malaysian food ingredient. Int. J. Syst. Evol. Microbiol. 50: 19-24.
16. Mazurier SS, Wernars K (1992). Typing of Listeria strain by randomly amplified DNA. Res. Microbiol. 143: 499-505.
17. Michel D, Robin D, Carine D, Jacqueline D, Emmanuel T, Philippe T (2008). In vitro detection and characterization of bacteriocinlike inhibitory activity of lactic acid bacteria (LAB) isolated from Senegalese local food products. Afr. J. Microbiol. Res. 2: 206-216.
18. Nigatu A (2000). Evaluation of numerical analyses of RAPD and API 50 CH patterns to differentiate Lactobacillus plantarum, Lact. fermentum, Lact. rhamnosus, Lact. sake, Lact. parabuchneri, Lact. gallinarum, Lact. casei, Weissella minor and related taxaisolated from kocho and tef. J. Appl. Microbiol. 89: 969-978.
19. Olubukola O (2003). Molecular techniques: An overview of methods for the detection of bacteria. Afr. J. Biotechnol. 12: 710-713.
20. Pot B, Hertel C, Ludwig W, Descheemaeker P, Kersters K, Schleifer KH (1993). Identification and classification of Lactobacil-lus acidophilus, Lb. gasseri and Lb. johnsonii strains by SDS-PAGE and rRNA targeted oligonucleotide probe hybridization. J. Gen. Microbiol. 139: 513-517.
21. Quiberoni A, Reinheimer JA (1998). Physicochemical characteri-zation of phage adsorption to Lactobacillus helveticus ATCC 15807 cells. J. Appl. Bacteriol. 85: 762-768.
22. Rodriguez E, Gonzales B, Gaya P, Nuez M, Medina M (2000). Diversity of bacteriocins produced by Lactic acid bacteria isolated from raw milk. Int. Dairy J. 10: 7-15.
23. Roushdy IM, Ehrmann A, Vogel RF (1998). Molecular identi-fication and characterization of halotolerant lactic acid bacteria isolated from soft pickled Domiati cheese. Food. Sci. (CMTL). 20: 40-45.
24. Sambrook J, Fritsch F, Maniatis T (1989). Molecular cloning: a laboratory manual, 2nd ed., vol.3. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
25. Schleifer KH, Ehrmann M, Beimfohr C, Brockman E, Ludwig W, Amann R (1995). Application of molecular methods for classification and identification of lactic acid bacteria. Int. Dairy J. 5: 1082-1094.
26. Sutherland I (1994). Structure-function relationships in microbial exopolysaccharieds. Biotechnol. Adv. 12: 393-448.