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Journal of the American Chemical Society
Volumn 131, Issue 32, 2009, Pages 11433-11441
Real-time electrochemical monitoring of the polymerase chain reaction by mediated redox catalysis
Author keywords

[No Author keywords available]
Indexed keywords

AMPLICONS; ANODIC POTENTIALS; BACTERIAL GENOMES; BIOLOGICAL TARGETS; BIPYRIDINES; CATALYTIC PEAK; COPY NUMBER; DETECTION LIMITS; DETECTION SCHEME; DNA PROBE; DNA PRODUCTS; DNA SYNTHESISS; DOWN-SCALING; ELECTROACTIVE; ELECTROCHEMICAL METHODS; ELECTROCHEMICAL MONITORING; FUNCTIONALIZATIONS; HIGH DYNAMIC RANGE; HUMAN CYTOMEGALOVIRUS; INDIRECT MEASUREMENTS; LINEAR CURVE; MINIATURIZED DEVICES; PCR AMPLIFICATION; PCR REACTIONS; POINT OF CARE; POLYMERASE CHAIN REACTION; PROOF OF PRINCIPLES; REAL-TIME PCR; REAL-WORLD; REDOX CATALYSIS; REDOX CATALYSTS; RU(BPY); SEMI-LOGARITHMIC PLOTS; SOLID-PHASE; THERMAL CYCLER; TRIPHOSPHATE; WORKING VOLUME;
EID: 68849091644     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja901368m     Document Type: Article
Times cited : (57)
References (59)
  • 37
    • 68849093391 scopus 로고    scopus 로고
    • It is worth to note that the main drawback of solid-phase PCR is the dimer formation through molecular crowding and primer interference, which result in poor PCR amplification efficiency with an average value between 1.0 and 1.4,36 whereas typical solution-phase amplification generally has an efficiency ranging from 1.6 to 2.0
    • It is worth to note that the main drawback of solid-phase PCR is the dimer formation through molecular crowding and primer interference, which result in poor PCR amplification efficiency with an average value between 1.0 and 1.4,36 whereas typical solution-phase amplification generally has an efficiency ranging from 1.6 to 2.0.
  • 47
    • 68849113948 scopus 로고    scopus 로고
    • Disposable carbon-based screen-printed electrodes are particularly well adapted for the goal of the work because they can be easily mass produced at low cost with a high degree of flexibility in the pattern design. Moreover, they possess low background current and good reproducibility
    • Disposable carbon-based screen-printed electrodes are particularly well adapted for the goal of the work because they can be easily mass produced at low cost with a high degree of flexibility in the pattern design. Moreover, they possess low background current and good reproducibility.
  • 49
    • 68849107065 scopus 로고    scopus 로고
    • 4 salts, respectively. The concentrations of these two latter additives were also optimized so as to have PCR yields as high as for standard Tris-HCl buffer.
    • 4 salts, respectively. The concentrations of these two latter additives were also optimized so as to have PCR yields as high as for standard Tris-HCl buffer.
  • 50
    • 68849118209 scopus 로고    scopus 로고
    • To avoid significant electrochemical consumption of the base throughout the repetitive cyclic voltammetric scans, the self-contained electrochemical cells were designed with a small electrode area/solution ratio of 0.4
    • To avoid significant electrochemical consumption of the base throughout the repetitive cyclic voltammetric scans, the self-contained electrochemical cells were designed with a small electrode area/solution volume ratio of 0.4.
  • 53
    • 68849109942 scopus 로고    scopus 로고
    • The programmed times for each temperature step of the cycle were made relatively long because of significant thermal inertia of the electrochemical cell. At 0.3 V s-1, it takes less than 5 s to record one voltammogram, so the overall cells of the array could be entirely scanned during the annealing step of each cycle
    • The programmed times for each temperature step of the cycle were made relatively long because of significant thermal inertia of the electrochemical cell. At 0.3 V s-1, it takes less than 5 s to record one voltammogram, so the overall cells of the array could be entirely scanned during the annealing step of each cycle.
  • 54
    • 68849083798 scopus 로고    scopus 로고
    • Among the different additives present in the commercialized DNA polymerase solution, surfactants used to stabilize the enzyme were suspected to affect the catalytic response by adsorption on the electrode surface. Unfortunately, in the absence of precise knowledge of constituents contained in the supplied proprietary enzyme solution, it was not possible to clearly identify the additive responsible for this phenomenon
    • Among the different additives present in the commercialized DNA polymerase solution, surfactants used to stabilize the enzyme were suspected to affect the catalytic response by adsorption on the electrode surface. Unfortunately, in the absence of precise knowledge of constituents contained in the supplied proprietary enzyme solution, it was not possible to clearly identify the additive responsible for this phenomenon.
  • 57
    • 68849112919 scopus 로고    scopus 로고
    • The use of a commercial buffer was rendered possible because at the low anodic potential at which the catalytic oxidation takes place, there was no significant interference of chlorides ions brought by the buffer
    • The use of a commercial buffer was rendered possible because at the low anodic potential at which the catalytic oxidation takes place, there was no significant interference of chlorides ions brought by the buffer.
  • 58
    • 68849087994 scopus 로고    scopus 로고
    • We have obtained a similar result with a positive sample in which we have voluntary not added the polymerase into the PCR mixture
    • We have obtained a similar result with a positive sample in which we have voluntary not added the polymerase into the PCR mixture.

* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.