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It is worth to note that the main drawback of solid-phase PCR is the dimer formation through molecular crowding and primer interference, which result in poor PCR amplification efficiency with an average value between 1.0 and 1.4,36 whereas typical solution-phase amplification generally has an efficiency ranging from 1.6 to 2.0
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Disposable carbon-based screen-printed electrodes are particularly well adapted for the goal of the work because they can be easily mass produced at low cost with a high degree of flexibility in the pattern design. Moreover, they possess low background current and good reproducibility
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Disposable carbon-based screen-printed electrodes are particularly well adapted for the goal of the work because they can be easily mass produced at low cost with a high degree of flexibility in the pattern design. Moreover, they possess low background current and good reproducibility.
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4 salts, respectively. The concentrations of these two latter additives were also optimized so as to have PCR yields as high as for standard Tris-HCl buffer.
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4 salts, respectively. The concentrations of these two latter additives were also optimized so as to have PCR yields as high as for standard Tris-HCl buffer.
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68849118209
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To avoid significant electrochemical consumption of the base throughout the repetitive cyclic voltammetric scans, the self-contained electrochemical cells were designed with a small electrode area/solution ratio of 0.4
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To avoid significant electrochemical consumption of the base throughout the repetitive cyclic voltammetric scans, the self-contained electrochemical cells were designed with a small electrode area/solution volume ratio of 0.4.
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The programmed times for each temperature step of the cycle were made relatively long because of significant thermal inertia of the electrochemical cell. At 0.3 V s-1, it takes less than 5 s to record one voltammogram, so the overall cells of the array could be entirely scanned during the annealing step of each cycle
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The programmed times for each temperature step of the cycle were made relatively long because of significant thermal inertia of the electrochemical cell. At 0.3 V s-1, it takes less than 5 s to record one voltammogram, so the overall cells of the array could be entirely scanned during the annealing step of each cycle.
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Among the different additives present in the commercialized DNA polymerase solution, surfactants used to stabilize the enzyme were suspected to affect the catalytic response by adsorption on the electrode surface. Unfortunately, in the absence of precise knowledge of constituents contained in the supplied proprietary enzyme solution, it was not possible to clearly identify the additive responsible for this phenomenon
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Among the different additives present in the commercialized DNA polymerase solution, surfactants used to stabilize the enzyme were suspected to affect the catalytic response by adsorption on the electrode surface. Unfortunately, in the absence of precise knowledge of constituents contained in the supplied proprietary enzyme solution, it was not possible to clearly identify the additive responsible for this phenomenon.
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The use of a commercial buffer was rendered possible because at the low anodic potential at which the catalytic oxidation takes place, there was no significant interference of chlorides ions brought by the buffer
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The use of a commercial buffer was rendered possible because at the low anodic potential at which the catalytic oxidation takes place, there was no significant interference of chlorides ions brought by the buffer.
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We have obtained a similar result with a positive sample in which we have voluntary not added the polymerase into the PCR mixture
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We have obtained a similar result with a positive sample in which we have voluntary not added the polymerase into the PCR mixture.
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