Early loss of mitochondrial inner transmembrane potential in khat-induced cell death of primary normal human oral cells

Toxicology

Volumn 263, Issue 2-3, 2009, Pages 108-116

  Lukandu, Ochiba M ^a,b   Bredholt, Therese ^a,b   Neppelberg, Evelyn ^b   Gjertsen, Bjørn T ^b   Johannessen, Anne C ^a   Vintermyr, Olav K ^a   Costea, Daniela Elena ^a  

^a HAUKELAND UNIVERSITY HOSPITAL   (Norway)

^b UNIVERSITY OF BERGEN   (Norway)

Author keywords

Apoptosis; Khat; Mitochondrial membrane potential; Oral

Indexed keywords

APOPTOSIS INDUCING FACTOR; BONGKREKIC ACID; CATHA EDULIS EXTRACT; CYCLOSPORIN A;

ARTICLE; CELL DEATH; CELL KINETICS; CELL NUCLEUS; CELL SURVIVAL; CHEMICAL CARCINOGENESIS; CONTROLLED STUDY; CYTOPATHOLOGY; CYTOSOL; FIBROBLAST CULTURE; HUMAN; HUMAN CELL; IN VITRO STUDY; KERATINOCYTE; MITOCHONDRIAL MEMBRANE POTENTIAL; MITOCHONDRIAL RESPIRATION; MOUTH MUCOSA; PRIORITY JOURNAL; PROTEIN SECRETION; PROTEIN TRANSPORT;

ADULT; APOPTOSIS INDUCING FACTOR; CATHA; CELL DEATH; CELL SURVIVAL; CELLS, CULTURED; CULTURE MEDIA, SERUM-FREE; FIBROBLASTS; HUMANS; KERATINOCYTES; KINETICS; MEMBRANE POTENTIAL, MITOCHONDRIAL; MOUTH MUCOSA;

CATHA EDULIS;

EID: 68549095947     PISSN: 0300483X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.tox.2009.06.024     Document Type: Article

References (30)

1. Al-Ahdal M.N., McGarry T.J., and Hannan M.A. Cytotoxicity of Khat (Catha edulis) extract on cultured mammalian cells: effects on macromolecule biosynthesis. Mutat. Res. 204 (1988) 317-322
2. Al-Habori M. The potential adverse effects of habitual use of Catha edulis (khat). Expert Opin. Drug Saf. 4 (2005) 1145-1154
3. Al-Motarreb A., Baker K., and Broadley K.J. Khat: pharmacological and medical aspects and its social use in Yemen. Phytother. Res. 16 (2002) 403-413
4. Al-Qirim T.M., Shahwan M., Zaidi K.R., Uddin Q., and Banu N. Effect of khat, its constituents and restraint stress on free radical metabolism of rats. J. Ethnopharmacol. 83 (2002) 245-250
5. Blom W.M., de Bont H.J., and Nagelkerke J.F. Regional loss of the mitochondrial membrane potential in the hepatocyte is rapidly followed by externalization of phosphatidylserines at that specific site during apoptosis. J. Biol. Chem. 278 (2003) 12467-12474
6. Carvalho F. The toxicological potential of khat. J. Ethnopharmacol. 87 (2003) 1-2
7. Costea D.E., Dimba A.O., Loro L.L., Vintermyr O.K., and Johannessen A.C. Proliferation and differentiation in organotypic serum free cultures of normal human oral mucosa. In: Varma A.K., and Kowalski L.P. (Eds). 8th International Conference on Oral Cancer (2002), Rajkamal Electric Press, Rio de Janeiro, Brazil 108-113
8. Costea D.E., Dimba A.O., Loro L.L., Vintermyr O.K., and Johannessen A.C. The phenotype of in vitro reconstituted normal human oral epithelium is essentially determined by culture medium. J. Oral Pathol. Med. 34 (2005) 247-252
9. Crompton M., Barksby E., Johnson N., and Capano M. Mitochondrial intermembrane junctional complexes and their involvement in cell death. Biochimie 84 (2002) 143-152
10. Dimba E.A., Gjertsen B.T., Bredholt T., Fossan K.O., Costea D.E., Francis G.W., Johannessen A.C., and Vintermyr O.K. Khat (Catha edulis)-induced apoptosis is inhibited by antagonists of caspase-1 and -8 in human leukaemia cells. Br. J. Cancer 91 (2004) 1726-1734
11. Galluzzi L., Zamzami N., de La Motte Rouge T., Lemaire C., Brenner C., and Kroemer G. Methods for the assessment of mitochondrial membrane permeabilization in apoptosis. Apoptosis 12 (2007) 803-813
12. Grimm S., and Brdiczka D. The permeability transition pore in cell death. Apoptosis 12 (2007) 841-855
13. Ichas F., Jouaville L.S., and Mazat J.P. Mitochondria are excitable organelles capable of generating and conveying electrical and calcium signals. Cell 89 (1997) 1145-1153
14. Joza N., Susin S.A., Daugas E., Stanford W.L., Cho S.K., Li C.Y., Sasaki T., Elia A.J., Cheng H.Y., Ravagnan L., Ferri K.F., Zamzami N., Wakeham A., Hakem R., Yoshida H., Kong Y.Y., Mak T.W., Zuniga-Pflucker J.C., Kroemer G., and Penninger J.M. Essential role of the mitochondrial apoptosis-inducing factor in programmed cell death. Nature 410 (2001) 549-554
15. Kassie F., Darroudi F., Kundi M., Schulte-Hermann R., and Knasmuller S. Khat (Catha edulis) consumption causes genotoxic effects in humans. Int. J. Cancer 92 (2001) 329-332
16. Kerr J.F., Wyllie A.H., and Currie A.R. Apoptosis: a basic biological phenomenon with wide-ranging implications in tissue kinetics. Br. J. Cancer 26 (1972) 239-257
17. Kroemer G., El-Deiry W.S., Golstein P., Peter M.E., Vaux D., Vandenabeele P., Zhivotovsky B., Blagosklonny M.V., Malorni W., Knight R.A., Piacentini M., Nagata S., and Melino G. Classification of cell death: recommendations of the Nomenclature Committee on Cell Death. Cell Death Differ. 12 Suppl. 2 (2005) 1463-1467
18. Kroemer G., Galluzzi L., and Brenner C. Mitochondrial membrane permeabilization in cell death. Physiol. Rev. 87 (2007) 99-163
19. Leist M., and Jaattela M. Four deaths and a funeral: from caspases to alternative mechanisms. Nat. Rev. Mol. Cell Biol. 2 (2001) 589-598
20. Lukandu O.M., Costea D.E., Neppelberg E., Johannessen A.C., and Vintermyr O.K. Khat (Catha edulis) induces reactive oxygen species and apoptosis in normal human oral keratinocytes and fibroblasts. Toxicol. Sci. 103 (2008) 311-324
21. Lukandu O.M., Costea D.E., Dimba E.A., Neppelberg E., Bredholt T., Gjertsen B.T., Vintermyr O.K., and Johannessen A.C. Khat induces G1-phase arrest and increased expression of stress-sensitive p53 and p16 proteins in normal human oral keratinocytes and fibroblasts. Eur. J. Oral Sci. 116 (2008) 23-30
22. Nasr A.H., and Khatri M.L. Head and neck squamous cell carcinoma in Hajjah, Yemen. Saudi Med J 21 (2000) 565-568
23. Pacher P., and Hajnoczky G. Propagation of the apoptotic signal by mitochondrial waves. EMBO J. 20 (2001) 4107-4121
24. Pastorino J.G., Tafani M., Rothman R.J., Marcinkeviciute A., Hoek J.B., and Farber J.L. Functional consequences of the sustained or transient activation by Bax of the mitochondrial permeability transition pore. J. Biol. Chem. 274 (1999) 31734-31739
25. Slater T.F., Sawyer B., and Straeuli U. Studies on succinate-tetrazolium reductase systems. III. Points of coupling of four different tetrazolium salts. Biochim. Biophys. Acta 77 (1963) 383-393
26. Soufi H.E., Kameswaran M., and Malatani T. Khat and oral cancer. J. Laryngol. Otol. 105 (1991) 643-645
27. Susin S.A., Daugas E., Ravagnan L., Samejima K., Zamzami N., Loeffler M., Costantini P., Ferri K.F., Irinopoulou T., Prevost M.C., Brothers G., Mak T.W., Penninger J., Earnshaw W.C., and Kroemer G. Two distinct pathways leading to nuclear apoptosis. J. Exp. Med. 192 (2000) 571-580
28. Tariq M., Parmar N.S., Qureshi S., el-Feraly F.S., and Al-Meshal I.A. Clastogenic evaluation of cathinone and amphetamine in somatic cells of mice. Mutat. Res. 190 (1987) 153-157
29. Toennes S.W., Harder S., Schramm M., Niess C., and Kauert G.F. Pharmacokinetics of cathinone, cathine and norephedrine after the chewing of khat leaves. Br. J. Clin. Pharmacol. 56 (2003) 125-130
30. Zamzami N., Marchetti P., Castedo M., Decaudin D., Macho A., Hirsch T., Susin S.A., Petit P.X., Mignotte B., and Kroemer G. Sequential reduction of mitochondrial transmembrane potential and generation of reactive oxygen species in early programmed cell death. J. Exp. Med. 182 (1995) 367-377