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Volumn 19, Issue 17, 2009, Pages 4937-4938

Antitubercular activity of α,ω-diaminoalkanes, H2N(CH2)nNH2

Author keywords

Diamines; Drugs; Ethambutol; Tuberculosis

Indexed keywords

ALPHA OMEGA DIAMINOALKANE DERIVATIVE; DIAMINE DERIVATIVE; ETHAMBUTOL; UNCLASSIFIED DRUG;

EID: 68549089180     PISSN: 0960894X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bmcl.2009.07.086     Document Type: Article
Times cited : (14)

References (16)
  • 11
    • 68549120770 scopus 로고    scopus 로고
    • note
    • 2, n = 2-12) was made directly on the plate. The final drug concentrations tested were 0.01-10.0 μL/mL. Plates were covered and sealed with parafilm and incubated at 37 °C for five days. After this time, 25 μL of a freshly prepared 1:1 mixture of Alamar Blue (Accumed International, Westlake Ohio) reagent and 10% tween 80 was added to the plate and incubated for 24 h. A blue color in the well was interpreted as no bacterial growth, and a pink color was scored as growth. The MIC (Minimal Inhibition Concentration) was defined as the lowest drug concentration, which prevented a color change from blue to pink.
  • 16
    • 68549117063 scopus 로고    scopus 로고
    • note
    • 6 UFC/well/100 μL) cultured in the presence of medium alone, tween 20 (3%) (live and dead controls, respectively) or different concentrations of compounds (1.0, 10.0 and 100 μg/mL) in a triplicate assay. After 48 h, stock MTT solution (5 mg/mL of saline; 20 mL/well) was added to the culture and 4 h later, the plate was centrifugate for 2 min at 2800 rpm, supernatant was discharged and Dimethyl sulfoxide (DMSO) (100 μL/well) was added for formazan crystals solubilization and the absorbance was read at 540 nm in a plate reader (Biorad-450).


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.