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note
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2, n = 2-12) was made directly on the plate. The final drug concentrations tested were 0.01-10.0 μL/mL. Plates were covered and sealed with parafilm and incubated at 37 °C for five days. After this time, 25 μL of a freshly prepared 1:1 mixture of Alamar Blue (Accumed International, Westlake Ohio) reagent and 10% tween 80 was added to the plate and incubated for 24 h. A blue color in the well was interpreted as no bacterial growth, and a pink color was scored as growth. The MIC (Minimal Inhibition Concentration) was defined as the lowest drug concentration, which prevented a color change from blue to pink.
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68549117063
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note
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6 UFC/well/100 μL) cultured in the presence of medium alone, tween 20 (3%) (live and dead controls, respectively) or different concentrations of compounds (1.0, 10.0 and 100 μg/mL) in a triplicate assay. After 48 h, stock MTT solution (5 mg/mL of saline; 20 mL/well) was added to the culture and 4 h later, the plate was centrifugate for 2 min at 2800 rpm, supernatant was discharged and Dimethyl sulfoxide (DMSO) (100 μL/well) was added for formazan crystals solubilization and the absorbance was read at 540 nm in a plate reader (Biorad-450).
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