Secreted expression of pseudozymogen forms of recombinant matriptase in Pichia pastoris

Enzyme and Microbial Technology

Volumn 45, Issue 4, 2009, Pages 288-294

  Mochida, Seiya ^a   Tsuzuki, Satoshi ^a   Yasumoto, Makoto ^a   Inouye, Kuniyo ^a   Fushiki, Tohru ^a  

^a KYOTO UNIVERSITY   (Japan)

Author keywords

Catalytic domain; Matriptase; Pichia pastoris; Type II transmembrane serine protease

Indexed keywords

AFTER-TREATMENT; CATALYTIC DOMAIN; CATALYTIC DOMAINS; CHROMOGENIC SUBSTRATE; CULTURE MEDIUM; EFFICIENT METHOD; EXPRESSION SYSTEM; HEXAHISTIDINE TAG; HYDROLYTIC ACTIVITIES; IN-VITRO; INTERMOLECULAR DISULFIDE BOND; MAMMALIAN CELLS; MATRIPTASE; METHYLOTROPHIC YEASTS; MULTIMERS; N-TERMINALS; PICHIA PASTORIS; SERINE PROTEASE; TRANSMEMBRANES; TYPE II TRANSMEMBRANE SERINE PROTEASE;

ANIMAL CELL CULTURE; CELL CULTURE; CELL MEMBRANES; DATA STORAGE EQUIPMENT; MAMMALS; YEAST;

AMINO ACIDS;

ENTEROPEPTIDASE; ENZYME PRECURSOR; HEXAHISTIDINE; MATRIPTASE; RECOMBINANT ENZYME;

AMINO TERMINAL SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; DISULFIDE BOND; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME PURIFICATION; NONHUMAN; PICHIA PASTORIS; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN SECRETION; QUANTITATIVE ANALYSIS;

MAMMALIA; PICHIA PASTORIS; VERTEBRATA;

EID: 68349158928     PISSN: 01410229     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.enzmictec.2009.06.008     Document Type: Article

References (22)

1. Lin C.Y., Anders J., Johnson M., Sang Q.A., and Dickson R.B. Molecular cloning of cDNA for matriptase, a matrix-degrading serine protease with trypsin-like activity. J Biol Chem 274 (1999) 18231-18236
2. Takeuchi T., Shuman M.A., and Craik C.S. Reverse biochemistry: use of macromolecular protease inhibitors to dissect complex biological processes and identify a membrane-type serine protease in epithelial cancer and normal tissue. Proc Natl Acad Sci USA 96 (1999) 11054-11061
3. Kim M.G., Chen C., Lyu M.S., Cho E.G., Park D., Kozak C., et al. Cloning and chromosomal mapping of a gene isolated from thymic stromal cells encoding a new mouse type II membrane serine protease, epithin, containing four LDL receptor modules and two CUB domains. Immunogenetics 49 (1999) 420-428
4. Satomi S., Yamasaki Y., Tsuzuki S., Hitomi Y., Iwanaga T., and Fushiki T. A role for membrane-type serine protease (MT-SP1) in intestinal epithelial turnover. Biochem Biophys Res Commun 287 (2001) 995-1002
5. Szabo R., and Bugge T.H. Type II transmembrane serine proteases in development and disease. Int J Biochem Cell Biol 40 (2008) 1297-1316
6. Oberst M.D., Williams C.A., Dickson R.B., Johnson M.D., and Lin C.Y. The activation of matriptase requires its noncatalytic domains, serine protease domain, and its cognate inhibitor. J Biol Chem 278 (2003) 26773-26779
7. Benaud C., Dickson R.B., and Lin C.Y. Regulation of the activity of matriptase on epithelial cell surfaces by a blood-derived factor. Eur J Biochem 268 (2001) 1439-1447
8. Lee S.L., Dickson R.B., and Lin C.Y. Activation of hepatocyte growth factor and urokinase/plasminogen activator by matriptase, an epithelial membrane serine protease. J Biol Chem 275 (2000) 36720-36725
9. Takeuchi T., Harris J.L., Huang W., Yan K.W., Coughlin S.R., and Craik C.S. Cellular localization of membrane-type serine protease 1 and identification of protease-activated receptor-2 and single-chain urokinase-type plasminogen activator as substrates. J Biol Chem 275 (2000) 26333-26342
10. Bugge T.H., List K., and Szabo R. Matriptase-dependent cell surface proteolysis in epithelial development and pathogenesis. Front Biosci 12 (2007) 5060-5070
11. Kojima K., Tsuzuki S., Fushiki T., and Inouye K. Roles of functional and structural domains of hepatocyte growth factor activator inhibitor type 1 in the inhibition of matriptase. J Biol Chem 283 (2008) 2478-2487
12. Kojima K., Tsuzuki S., Fushiki T., and Inouye K. The activity of a type II transmembrane serine protease, matriptase, is dependent solely on the catalytic domain. Biosci Biotechnol Biochem 73 (2009) 454-456
13. Yamasaki Y., Satomi S., Murai N., Tsuzuki S., and Fushiki T. Inhibition of membrane-type serine protease 1/matriptase by natural and synthetic protease inhibitors. J Nutr Sci Vitaminol (Tokyo) 49 (2003) 27-32
14. Tsuzuki S., Kokado Y., Satomi S., Yasamaki Y., Hirayasu H., Iwanaga T., et al. Purification and identification of a binding protein for pancreatic secretory trypsin inhibitor: a novel role of the inhibitor as an anti-granzyme A. Biochem J 372 (2003) 227-233
15. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227 (1970) 680-685
16. Tsuzuki S., Murai N., Miyake Y., Inouye K., Hirayasu H., Iwanaga T., et al. Evidence for the occurrence of membrane-type serine protease 1/matriptase on the basolateral sides of enterocytes. Biochem J 388 (2005) 679-687
17. Friedrich R., Fuentes-Prior P., Ong E., Coombs G., Hunter M., Oehler R., et al. Catalytic domain structures of MT-SP1/matriptase, a matrix-degrading transmembrane serine proteinase. J Biol Chem 277 (2002) 2160-2168
18. List K., Currie B., Scharschmidt T.C., Szabo R., Shireman J., Molinolo A., et al. Autosomal ichthyosis with hypotrichosis syndrome displays low matriptase proteolytic activity and is phenocopied in ST14 hypomorphic mice. J Biol Chem 282 (2007) 36714-36723
19. Hirayasu H., Yoshikawa Y., Tsuzuki S., and Fushiki T. Sulfated polysaccharides derived from dietary seaweeds increase the esterase activity of a lymphocyte tryptase, granzyme A. J Nutr Sci Vitaminol (Tokyo) 51 (2005) 475-477
20. Hirayasu H., Yoshikawa Y., Tsuzuki S., and Fushiki T. A role of a lymphocyte tryptase, granzyme A, in experimental ulcerative colitis. Biosci Biotechnol Biochem 71 (2007) 234-237
21. Hirayasu H., Yoshikawa Y., Tsuzuki S., and Fushiki T. A lymphocyte serine protease granzyme A causes detachment of a small-intestinal epithelial cell line (IEC-6). Biosci Biotechnol Biochem 72 (2008) 2294-2302
22. Yoshikawa Y., Hirayasu H., Tsuzuki S., and Fushiki T. Carrageenan inhibits granzyme A-induced detachment of and interleukin-8 release from alveolar epithelial A549 cells. Cytotechnology 58 (2008) 63-67