Studying the salt dependence of the binding of σ70 and σ32 to core RNA polymerase using luminescence resonance energy transfer

PLoS ONE

Volumn 4, Issue 8, 2009, Pages

  Glaser, Bryan T ^a,b   Bergendahl, Veit ^a,c   Anthony, Larry C ^a,d   Olson, Brian ^a,e   Burgess, Richard R ^a  

^a UNIVERSITY OF WISCONSIN MADISON   (United States)

^b SRI INTERNATIONAL   (United States)

^c ROCHE DIAGNOSTICS GMBH   (Germany)

^d DUPONT COMPANY   (United States)

^e UNIVERSITY OF WISCONSIN   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CYSTEINE; GLUTAMIC ACID; LYSINE; MUTANT PROTEIN; RNA POLYMERASE; SIGMA FACTOR; SIGMA FACTOR RPOD; SIGMA FACTOR RPOH; SODIUM CHLORIDE; UNCLASSIFIED DRUG; DNA DIRECTED RNA POLYMERASE; FLUORESCENT DYE; GLUTAMIC ACID DERIVATIVE; HEAT SHOCK PROTEIN; HEAT SHOCK SIGMA FACTOR 32; HEAT-SHOCK SIGMA FACTOR 32; RNA POLYMERASE SIGMA 70;

ARTICLE; BINDING AFFINITY; BINDING ASSAY; CHEMICAL LABELING; CONTROLLED STUDY; ESCHERICHIA COLI; FLUORESCENCE; LUMINESCENCE RESONANCE ENERGY TRANSFER; NONHUMAN; PROTEIN ANALYSIS; PROTEIN PROTEIN INTERACTION; PROTEIN RNA BINDING; CHEMISTRY; ENERGY TRANSFER; LUMINESCENCE; METABOLISM; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN BINDING; ULTRAVIOLET SPECTROPHOTOMETRY;

DNA-DIRECTED RNA POLYMERASES; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENERGY TRANSFER; FLUORESCENT DYES; GLUTAMATES; HEAT-SHOCK PROTEINS; LUMINESCENCE; PROTEIN BINDING; SIGMA FACTOR; SODIUM CHLORIDE; SPECTROPHOTOMETRY, ULTRAVIOLET;

EID: 68149122340     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0006490     Document Type: Article

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