Luminescence resonance energy transfer-based high-throughput screening assay for inhibitors of essential protein-protein interactions in bacterial RNA polymerase

Applied and Environmental Microbiology

Volumn 69, Issue 3, 2003, Pages 1492-1498

  Bergendahl, Veit ^a   Heyduk, Tomasz ^b   Burgess, Richard R ^a  

^a UNIVERSITY OF WISCONSIN MADISON   (United States)

^b SAINT LOUIS UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACIDS; ENERGY TRANSFER; LUMINESCENCE; PROTEINS; RNA;

PROTEIN BINDING;

BACTERIA;

BACTERIAL ENZYME; EUROPIUM; PROTEIN SUBUNIT; RNA POLYMERASE; SIGMA FACTOR;

LUMINESCENCE;

ARTICLE; ENERGY TRANSFER; ESCHERICHIA COLI; NONHUMAN; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN PROTEIN INTERACTION; SCREENING;

ANIMALS; BINDING SITES; DNA-DIRECTED RNA POLYMERASES; ENERGY TRANSFER; ESCHERICHIA COLI; LANTHANOID SERIES ELEMENTS; PORIFERA; PROTEIN BINDING; SEAWATER; SIGMA FACTOR; TRANSCRIPTION, GENETIC;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; EUKARYOTA; NEGIBACTERIA;

EID: 0344837844     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.69.3.1492-1498.2003     Document Type: Article

References (22)

1. Allison, L. A. 2000. The role of sigma factors in plastid transcription. Biochimie 82:537-548.
2. Arthur, T. M. 2000. Localization and characterization of a sigma subunit-binding site on the Escherichia coli RNA polymerase core enzyme. Ph.D. thesis. University of Wisconsin, Madison.
3. 70 binding site located on Escherichia coli core RNA polymerase. J. Biol. Chem. 275:23113-23119.
4. 70 binding site on the N terminus of the Escherichia coli RNA polymerase β′subunit. J. Biol. Chem. 273:31381-31387.
5. Bergendahl, V., L. C. Anthony, T. Heyduk, and R. R. Burgess. 2002. Oncolumn tris(2-carboxyethyl)phosphine reduction and IC5-maleimide labeling during purification of a RpoC fragment on a nickel-nitrilotriacetic acid column. Anal. Biochem. 307:368-374.
6. Burgess, R. R., T. M. Arthur, and B. C. Pietz. 2000. Mapping protein-protein interaction domains using ordered fragment ladder far-western analysis of hexahistidine-tagged fusion proteins. Methods Enzymol. 328:141-157.
7. 70 subunit of Escherichia coli RNA polymerase and the nontemplate strand oligonucleotide. Luminescence resonance energy transfer study. J. Biol. Chem. 274:3315-3322.
8. Heyduk, T. 2001. Luminescence resonance energy transfer analysis of RNA polymerase complexes. Methods 25:44-53.
9. Landick, R., D. Wang, and C. L. Chan. 1996. Quantitative analysis of transcriptional pausing by Escherichia coli RNA polymerase: his leader pause site as paradigm. Methods Enzymol. 274:334-353.
10. Lesley, S. A., and R. R. Burgess. 1989. Characterization of the Escherichia coli transcription factor sigma 70: localization of a region involved in the interaction with core RNA polymerase. Biochemistry 28:7728-7734.
11. Lonetto, M., M. Gribskov, and C. A. Gross. 1992. The 0.70 family: sequence conservation and evolutionary relationships. J. Bacteriol. 174:3843-3849.
12. Lonetto, M. A., V. Rhodius, K. Lamberg, P. Kiley, S. Busby, and C. Gross. 1998. Identification of a contact site for different transcription activators in region 4 of the Escherichia coli RNA polymerase 0.70 subunit. J. Mol. Biol. 284:1353-1365.
13. Mittenhuber, G. 2002. An inventory of genes encoding RNA polymerase sigma factors in 31 completely sequenced eubacterial genomes. J. Mol. Microbiol. Biotechnol. 4:77-91.
14. Murakami, K. S., S. Masuda, and S. A. Darst. 2002. Structural basis of transcription initiation: RNA polymerase holoenzyme at 4 Å resolution. Science 296:1280-1284.
15. Selvin, P. R. 1995. Fluorescence resonance energy transfer. Methods Enzymol. 246:300-334.
16. Selvin, P. R. 1999. Luminescent lanthanide chelates for improved resonance energy transfer and application to biology, p. 457-487. In W. Rettig, B. Strehmel, and S. Schrader (ed.), Applied fluorescence in chemistry, biology and medicine, 1st ed. Springer-Verlag, Berlin, Germany.
17. Selvin, P. R. 2000. The renaissance of fluorescence resonance energy transfer. Nat. Struct. Biol. 7:730-734.
18. Stryer, L. 1978. Fluorescence energy transfer as a spectroscopic ruler. Annu. Rev. Biochem. 47:819-846.
19. Tanaka, J., G. Marriott, and T. Higa. 2001. Cacofurans A and B, new furanoditerpenes from a marine sponge. J. Nat. Prod. 64:1468-1470.
20. Thompson, N. E., D. A. Hager, and R. R. Burgess. 1992. Isolation and characterization of a polyol-responsive monoclonal antibody useful for gentle purification of Escherichia coli RNA polymerase. Biochemistry 31:7003-7008.
21. Tracy, R. L., and D. B. Stern. 1995. Mitochondrial transcription initiation: promoter structures and RNA polymerases. Curr. Genet. 28:205-216.
22. Vassylyev, D. G., S. Sekine, O. Laptenko, J. Lee, M. N. Vassylyeva, S. Borukhov, and S. Yokoyama. 2002. Crystal structure of a bacterial RNA polymerase holoenzyme at 2.6 Å resolution. Nature 417:712-719.