Mechanism of interaction between the general anesthetic halothane and a model ion channel protein, II: Fluorescence and vibrational spectroscopy using a cyanophenylalanine probe

Biophysical Journal

Volumn 96, Issue 10, 2009, Pages 4176-4187

  Liu, Jing ^a   Strzalka, Joseph ^a   Tronin, Andrey ^a   Johansson, Jonas S ^a   Blasie, J Kent ^a  

^a UNIVERSITY OF PENNSYLVANIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALANINE; CYANOPHENYLALANINE; DETERGENT; HALOTHANE; ION CHANNEL; MEMBRANE PROTEIN; NITRILE; PHENYLALANINE DERIVATIVE; TRYPTOPHAN; UNCLASSIFIED DRUG; WATER; ANESTHETIC AGENT; BUFFER; DRUG DERIVATIVE; FLUORESCENT DYE; P CYANOPHENYLALANINE; P-CYANOPHENYLALANINE; PEPTIDE;

ABSORPTION; AIR; ALPHA HELIX; AQUEOUS SOLUTION; ARTICLE; BINDING SITE; CONCENTRATION (PARAMETERS); CONFORMATION; DIFFUSION; ENVIRONMENT; FILM; FLUORESCENCE; MODEL; MOLECULAR DYNAMICS; MUTANT; MUTATION; OSCILLATOR; PROTEIN INTERACTION; SIMULATION; SPECTROSCOPY; VIBRATION; AMINO ACID SEQUENCE; CHEMISTRY; CIRCULAR DICHROISM; INFRARED SPECTROPHOTOMETRY; METABOLISM; MOLECULAR GENETICS; PROTEIN BINDING; PROTEIN SECONDARY STRUCTURE; SPECTROFLUOROMETRY; SURFACE PROPERTY; SYNTHESIS; X RAY;

AIR; ALANINE; AMINO ACID SEQUENCE; ANESTHETICS, GENERAL; BUFFERS; CIRCULAR DICHROISM; DETERGENTS; FLUORESCENT DYES; HALOTHANE; ION CHANNELS; MOLECULAR SEQUENCE DATA; NITRILES; PEPTIDES; PROTEIN BINDING; PROTEIN STRUCTURE, SECONDARY; SPECTROMETRY, FLUORESCENCE; SPECTROPHOTOMETRY, INFRARED; SURFACE PROPERTIES; VIBRATION; WATER; X-RAYS;

EID: 68049123307     PISSN: 00063495     EISSN: 15420086     Source Type: Journal    
DOI: 10.1016/j.bpj.2009.01.055     Document Type: Article

References (28)

1. Eckenhoff, R. G., and J. S. Johansson. 1997. Molecular interactions between inhaled anesthetics and proteins. Pharmacol. Rev. 49:343-367.
2. Franks, N. P., and W. R. Lieb. 1994. Molecular and cellular mechanisms of general-anesthesia. Nature. 367:607-614.
3. Ye, S. X., J. Strzalka, I. Y. Churbanova, S. Y. Zheng, J. S. Johansson, et al. 2004. A model membrane protein for binding volatile anesthetics. Biophys. J. 87:4065-4074.
4. Strzalka, J., J. Liu, A. Tronin, I. Y. Churbanova, J. S. Johansson, et al. 2009. Mechanism of interaction between the general anesthetic halothane and a model ion channel protein, I: structural investigations via x-ray reflectivity from Langmuir monolayers. Biophys. J. 96:4164-4175.
5. Churbanova, I. Y., A. Tronin, J. Strzalka, T. Gog, I. Kuzmenko, et al. 2006. Monolayers of a model anesthetic-binding membrane protein: formation, characterization, and halothane-binding affinity. Biophys. J. 90:3255-3266.
6. Johansson, J. S., B. R. Gibney, F. Rabanal, K. S. Reddy, and P. L. Dutton. 1998. A designed cavity in the hydrophobic core of a four-alpha-helix bundle improves volatile anesthetic binding affinity. Biochemistry. 37:1421-1429.
7. Barth, A., and C. Zscherp. 2002. What vibrations tell us about proteins. Q. Rev. Biophys. 35:369-430.
8. Yoshikawa, S., D. H. Okeeffe, and W. S. Caughey. 1985. Investigations of cyanide as an infrared probe of hemeprotein ligand-binding sites. J. Biol. Chem. 260:3518-3528.
9. Getahun, Z., C. Y. Huang, T. Wang, B. De Leon, W. F. DeGrado, et al. 2003. Using nitrile-derivatized amino acids as infrared probes of local environment. J. Am. Chem. Soc. 125:405-411.
10. Tucker, M. J., Z. Getahun, V. Nanda, W. F. DeGrado, and F. Gai. 2004. A new method for determining the local environment and orientation of individual side chains of membrane-binding peptides. J. Am. Chem. Soc. 126:5078-5079.
11. Mukherjee, S., P. Chowdhury, W. F. DeGrado, and F. Gai. 2007. Site-specific hydration status of an amphipathic peptide in AOT reverse micelles. Langmuir. 23:11174-11179.
12. Reimers, J. R., and L. E. Hall. 1999. The solvation of acetonitrile. J. Am. Chem. Soc. 121:3730-3744.
13. Tucker, M. J., R. Oyola, and F. Gai. 2005. Conformational distribution of a 14-residue peptide in solution: A fluorescence resonance energy transfer study. J. Phys. Chem. B. 109:4788-4795.
14. Johansson, J. S., R. G. Eckenhoff, and P. L. Dutton. 1995. Binding of halothane to serum-albumin demonstrated using tryptophan fluorescence. Anesthesiology. 83:316-324.
15. Tatulian, S. A., and L. K. Tamm. 2000. Secondary structure, orientation, oligomerization, and lipid interactions of the transmembrane domain of influenza hemagglutinin. Biochemistry. 39:496-507.
16. Luo, P. Z., and R. L. Baldwin. 1997. Mechanism of helix induction by trifluoroethanol: A framework for extrapolating the helix-forming properties of peptides from trifluoroethanol/water mixtures back to water. Biochemistry. 36:8413-8421.
17. Holtom, G. R. 1990. Artifacts and diagnostics in fast fluorescence measurements. Proc. SPIE. 1204:2-12.
18. Vigano, C., L. Manciu, F. Buyse, E. Goormaghtigh, and J. M. Ruysschaert. 2000. Attenuated total reflection IR spectroscopy as a tool to investigate the structure, orientation and tertiary structure changes in peptides and membrane proteins. Biopolymers. 55:373-380.
19. Tamm, L. K., and S. A. Tatulian. 1993. Orientation of functional and nonfunctional pts permease signal sequences in lipid bilayers - a polarized attenuated total-reflection infrared study. Biochemistry. 32:7720-7726.
20. Yamamoto, K., and A. Masui. 1995. Complex refractive-index determination of bulk materials from infrared reflection spectra. Appl. Spectrosc. 49:639-644.
21. Johansson, J. S., D. Scharf, L. A. Davies, K. S. Reddy, and R. G. Eckenhoff. 2000. A designed four-alpha-helix bundle that binds the volatile general anesthetic halothane with high affinity. Biophys. J. 78:982-993.
22. Zheng, S., J. Strzalka, D. H. Jones, S. J. Opella, and J. K. Blasie. 2003. Comparative structural studies of Vpu peptides in phospholipid monolayers by x-ray scattering. Biophys. J. 84:2393-2415.
23. Crick, F. H. C. 1953. The packing of alpha-helices - simple coiled-coils. Acta Crystallogr. 6:689-697.
24. Crick, F. H. C. 1953. The Fourier transform of a coiled-coil. Acta Crystallogr. 6:685-689.
25. Tucker, M. J., R. Oyola, and F. Gai. 2006. A novel fluorescent probe for protein binding and folding studies: p-cyano-phenylalanine. Biopolymers. 83:571-576.
26. Borman, V. D., B. Johansson, N. V. Skorodumova, I. V. Tronin, V. N. Tronin, et al. 2006. Diffusion and particle mobility in 1D system. Phys. Lett. A. 359:504-508.
27. Blasie, J. K., S. Zheng, and J. Strzalka. 2003. Solution to the phase problem for specular x-ray or neutron reflectivity from thin films on liquid surfaces. Phys. Rev. B. 67:224201.
28. Zou, H. L., J. Liu, and J. K. Blasie. 2009. Mechanism of interaction between the general anesthetic halothane and a model ion channel protein, III: molecular dynamics simulation incorporating a cyanophenylalanine spectroscopic probe. Biophys. J. 96:4188-4199.