Secretory expression and purification of recombinant escherichia coli heat-Labile enterotoxin B subunit and its applications on intranasal vaccination of hantavirus

Molecular Biotechnology

Volumn 41, Issue 2, 2009, Pages 91-98

  Cao, Shouchun ^a,c   Zhang, Ying ^b   Liu, Feng ^c   Wang, Qin ^c   Zhang, Quanfu ^c   Liu, Qinzhi ^c   Li, Chuan ^c   Liang, Mifang ^c   Li, Dexin ^c  

^a NATIONAL INSTITUTE FOR THE CONTROL OF PHARMACEUTICAL AND BIOLOGICAL PRODUCTS   (China)

^b SHANDONG UNIVERSITY   (China)

^c CHINESE CENTER FOR DISEASE CONTROL AND PREVENTION   (China)

Author keywords

Hantavirus; Intranasal vaccination; Recombinant heat labile enterotoxin B subunit; Secretory expression

Indexed keywords

BACTERIAL PROTEIN; BINDING ACTIVITIES; BIOLOGICAL ACTIVITIES; E. COLI; FUSION PROTEINS; HANTAVIRUS; HEAT-LABILE ENTEROTOXIN; HIS-TAG; HUMORAL IMMUNE RESPONSE; INTRANASAL; INTRANASAL VACCINATION; PATHOGENIC E. COLI; PERIPLASMIC SPACE; PROKARYOTIC EXPRESSION; RECOMBINANT ESCHERICHIA COLI; RECOMBINANT HEAT-LABILE ENTEROTOXIN B SUBUNIT; SECRETORY EXPRESSION; SIGNAL PEPTIDE;

AFFINITY CHROMATOGRAPHY; AMINES; CHROMATOGRAPHIC ANALYSIS; ESCHERICHIA COLI; NUCLEIC ACIDS; PURIFICATION; SUGARS;

DRUG PRODUCTS;

BACTERIAL PROTEIN; ENTEROTOXIN; GANGLIOSIDE; IMMUNOGLOBULIN A; IMMUNOGLOBULIN G; LABILE ENTEROTOXIN B; LACTOSE; NICKEL; NUCLEOPROTEIN; PELB SIGNAL PEPTIDE; POLYHISTIDINE TAG; SECRETORY IMMUNOGLOBULIN; SIGNAL PEPTIDE; UNCLASSIFIED DRUG; VIRUS FUSION PROTEIN; VIRUS VACCINE;

AFFINITY CHROMATOGRAPHY; ARTICLE; BACTERIAL GENE; BINDING AFFINITY; BIOLOGICAL ACTIVITY; COMPARATIVE STUDY; CONTROLLED STUDY; CYTOPLASM; ENZYME LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; EXPRESSION VECTOR; FEMALE; HANTAVIRUS; HEAT; HUMORAL IMMUNITY; MOUSE; MUCOSAL IMMUNITY; NONHUMAN; NUCLEOTIDE SEQUENCE; PROKARYOTE; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; PROTEIN PURIFICATION; VACCINATION; VIRUS INFECTION;

ADMINISTRATION, INTRANASAL; ANIMALS; BACTERIAL TOXINS; CHROMATOGRAPHY, AFFINITY; CLONING, MOLECULAR; ENTEROTOXIGENIC ESCHERICHIA COLI; ENTEROTOXINS; ENZYME-LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; FEMALE; G(M1) GANGLIOSIDE; HANTAVIRUS; MICE; MICE, INBRED C57BL; RECOMBINANT PROTEINS; VACCINATION; VIRAL VACCINES;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; HANTAVIRUS; PROKARYOTA;

EID: 67749127447     PISSN: 10736085     EISSN: None     Source Type: Journal    
DOI: 10.1007/s12033-008-9101-4     Document Type: Article

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