Scanning mutagenesis of α-conotoxin Vc1.1 reveals residues crucial for activity at the α9α10 nicotinic acetylcholine receptor

Journal of Biological Chemistry

Volumn 284, Issue 30, 2009, Pages 20275-20284

  Halai, Reena ^a   Clark, Richard J ^a   Nevin, Simon T ^a   Jensen, Jonas E ^a   Adams, David J ^a   Craik, David J ^a  

^a UNIVERSITY OF QUEENSLAND   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

ANALGESIC DRUGS; BIOLOGICAL FUNCTIONS; CONOTOXIN; ELECTROPHYSIOLOGICAL STUDIES; FUNCTIONAL ACTIVITIES; FURTHER DEVELOPMENT; NEUROPATHIC PAIN; NICOTINIC ACETYLCHOLINE RECEPTORS; NICOTINIC ACETYLCHOLINE RECEPTORS (NACHR); NMR SPECTROSCOPY; PEPTIDE INHIBITORS; SECOND GENERATION; THERAPEUTIC TARGETS;

AMINES; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY;

TOBACCO;

ACETYLCHOLINE; ALANINE; ALPHA CONOTOXIN VC1.1; ALPHA9ALPHA10 NICOTINIC ACETYLCHOLINE RECEPTOR; AMINO ACID; ANALGESIC AGENT; ARGININE; ASPARTIC ACID; CONOTOXIN; CYSTEINE; D 11A; D 5A; GLYCYLCYSTYLCYSTYLALANYLASPARTYLPROLYLCYSTYLASPARAGYLTYROSYLASPARTYLHISTIDYLPROLYLGLUTAMYLISOLEUCYLCYSTEINE; GLYCYLCYSTYLCYSTYLARGINYLASPARTYLPROLYLARGINYLCYSTYLASPARAGYLTYROSYLASPARTYLHISTIDYLPROLYLGLUTAMYLISOLEUCYLCYSTEINE; GLYCYLCYSTYLCYSTYLLYSYLASPARTYLPROLYLARGINYLCYSTYLALANYLTYROSYLASPARTYLHISTIDYLPROLYLGLUTAMYLISOLEUCYLCYSTEINE; GLYCYLCYSTYLCYSTYLLYSYLASPARTYLPROLYLARGINYLCYSTYLASPARAGYLTYROSYLALANYLHISTIDYLPROLYLGLUTAMYLISOLEUCYLCYSTEINE; GLYCYLCYSTYLCYSTYLLYSYLASPARTYLPROLYLARGINYLCYSTYLASPARAGYLTYROSYLASPARTYLHISTIDYLPROLYLGLUTAMYLISOLEUCYLCYSTEINE; GLYCYLCYSTYLCYSTYLLYSYLASPARTYLPROLYLARGINYLCYSTYLLASPARAGINYLALANYLASPARTIDYLLHISTIDYLPROLYLGLUTAMYLISOLEUCYLCYSTEINE; GLYCYLCYSTYLCYSTYLSERYLALANYLPROLYLCYSTYLALANYLTYROSYLASPARTYLHISTIDYLPROLYLGLUTAMYLISOLEUCYLCYSTEINE; GLYCYLCYSTYLCYSTYLSERYLASPARTYLALANYLAGINYLCYSTYLASPARAGYLTYROSYLASPARTYLHISTIDYLPROLYLGLUTAMYLISOLEUCYLCYSTEINE; GLYCYLCYSTYLCYSTYLSERYLASPARTYLPROLYLALANYLCYSTYLASPARAGYLTYROSYLASPARTYLHISTIDYLPROLYLGLUTAMYLISOLEUCYLCYSTEINE; GLYCYLCYSTYLCYSTYLSERYLASPARTYLPROLYLARGINYLCYSTYLASPARAGINYLALANYLASPARTYLHISTIDYLPROLYLGLUTAMYLISOLEUCYLCYSTEINE; GLYCYLCYSTYLCYSTYLSERYLASPARTYLPROLYLARGINYLCYSTYLGLYCYLTYROSYLASPARTYLHISTIDYLPROLYLGLUTAMYLISOLEUCYLCYSTEINE; GLYCYLCYSTYLCYSTYLSERYLASPARTYLPROLYLARGINYLCYSTYLISOLEUCYLTYROSYLASPARTYLHISTIDYLPROLYLGLUTAMYLISOLEUCYLCYSTEINE; GLYCYLCYSTYLCYSTYLSERYLASPARTYLPROLYLARGINYLCYSTYLLYSYLCYLTYROSYLASPARTYLHISTIDYLPROLYLGLUTAMYLISOLEUCYLCYSTEINE; GLYCYLCYSTYLCYSTYLSERYLASPARTYLPROLYLCYSTYLALANYLTYROSYLASPARTYLHISTIDYLPROLYLGLUTAMYLISOLEUCYLCYSTEINE; ISOLEUCINE; LYSINE; N 9A; N 9G; N 9I; N 9L; NICOTINIC RECEPTOR; P 6A; PROLINE; R 7A; RECEPTOR SUBTYPE; S 4A; S 4K; S 4R; UNCLASSIFIED DRUG; UNINDEXED DRUG; Y 10A;

AMINO ACID SUBSTITUTION; ANIMAL CELL; ARTICLE; CONTROLLED STUDY; DRUG DETERMINATION; DRUG RECEPTOR BINDING; DRUG STRUCTURE; DRUG SYNTHESIS; DRUG TARGETING; ENZYME ACTIVITY; MEMBRANE CURRENT; MUTAGENESIS; MUTATIONAL ANALYSIS; NEUROPATHIC PAIN; NONHUMAN; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PRIORITY JOURNAL; PROTEIN FUNCTION; STRUCTURE ACTIVITY RELATION;

AMINO ACID SEQUENCE; ANIMALS; CONOTOXINS; CONUS SNAIL; HUMANS; HYDROGEN-ION CONCENTRATION; MAGNETIC RESONANCE SPECTROSCOPY; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; NICOTINIC ANTAGONISTS; OOCYTES; OXIDATION-REDUCTION; POINT MUTATION; RATS; RECEPTORS, NICOTINIC; STRUCTURE-ACTIVITY RELATIONSHIP; TITRIMETRY; XENOPUS;

RATTUS;

EID: 67749118310     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M109.015339     Document Type: Article

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