Spr1630 is responsible for the lethality of clpX mutations in Streptococcus pneumoniae

Journal of Bacteriology

Volumn 191, Issue 15, 2009, Pages 4888-4895

  Piotrowski, Andrew ^a   Burghout, Peter ^b   Morrison, Donald A ^a  

^a UNIVERSITY OF ILLINOIS AT CHICAGO   (United States)

^b RADBOUD UNIVERSITY MEDICAL CENTER   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSINE TRIPHOSPHATASE; BACTERIAL PROTEIN; CLP PROTEINASE; PROTEIN SPR1630; UNCLASSIFIED DRUG;

ARTICLE; BACTERIAL GENE; CCLA GENE; CLPX GENE; DNA BINDING; FRAMESHIFT MUTATION; GENE DELETION; GENE INSERTION; GENE MUTATION; LACTOBACILLUS JOHNSONII; LACTOBACILLUS RHAMNOSUS; LACTOCOCCUS; LETHAL MUTANT; LISTERIA MONOCYTOGENES; NONHUMAN; POINT MUTATION; PRIORITY JOURNAL; PROTEIN BINDING; SEQUENCE HOMOLOGY; STAPHYLOCOCCUS AUREUS; STREPTOCOCCUS; STREPTOCOCCUS PNEUMONIAE; TRANSCRIPTION REGULATION; TRPA GENE;

ADENOSINE TRIPHOSPHATASES; BACTERIAL PROTEINS; MODELS, GENETIC; MUTATION; POLYMERASE CHAIN REACTION; STREPTOCOCCUS PNEUMONIAE;

LACTOBACILLUS JOHNSONII; LACTOBACILLUS RHAMNOSUS; LACTOCOCCUS; LISTERIA MONOCYTOGENES; STAPHYLOCOCCUS AUREUS; STREPTOCOCCUS PNEUMONIAE;

EID: 67749117922     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.00285-09     Document Type: Article

References (49)

1. Ahlawat, S., and D. A. Morrison. 2009. ClpXP degrades SsrA-tagged proteins in Streptococcus pneumoniae. J. Bacteriol. 191:2894-2898.
2. Bijlsma, J. J., P. Burghout, T. G. Kloosterman, H. J. Bootsma, A. de Jong, P. W. Hermans, and O. P. Kuipers. 2007. Development of genomic array footprinting for identification of conditionally essential genes in Streptococcus pneumoniae. Appl. Environ. Microbiol. 73:1514-1524.
3. Burghout, P., H. J. Bootsma, T. G. Kloosterman, J. J. Bijlsma, C. E. de Jongh, O. P. Kuipers, and P. W. Hermans. 2007. Search for genes essential for pneumococcal transformation: the RADA DNA repair protein plays a role in genomic recombination of donor DNA. J. Bacteriol. 189:6540-6550.
4. Cato, A., and W. R. Guild. 1968. Transformation and DNA size. I. Activity of fragments of defined size and a fit to a random double cross-over model. J. Mol. Biol. 37:157-178.
5. Clarke, A. K., J. Schelin, and J. Porankiewicz. 1998. Inactivation of the clpP1 gene for the proteolytic subunit of the ATP-dependent Clp protease in the cyanobacterium Synechococcus limits growth and light acclimation. Plant Mol. Biol. 37:791-801.
6. Finn, R. D., J. Tate, J. Mistry, P. C. Coggill, J. S. Sammut, H. R. Hotz, G. Ceric, K. Forslund, S. R. Eddy, E. L. Sonnhammer, and A. Bateman. 2008. The Pfam protein families database. Nucleic Acids Res. 36:D281-D288.
7. Frees, D., and H. Ingmer. 1999. ClpP participates in the degradation of mis-folded protein in Lactococcus lactis. Mol. Microbiol. 31:79-87.
8. Frees, D., S. N. Qazi, P. J. Hill, and H. Ingmer. 2003. Alternative roles of ClpX and ClpP in Staphylococcus aureus stress tolerance and virulence. Mol. Microbiol. 48:1565-1578.
9. Frees, D., K. Savijoki, P. Varmanen, and H. Ingmer. 2007. Clp ATPases and ClpP proteolytic complexes regulate vital biological processes in low GC, gram-positive bacteria. Mol. Microbiol. 63:1285-1295.
10. Frees, D., K. Sørensen, and H. Ingmer. 2005. Global virulence regulation in Staphylococcus aureus: pinpointing the roles of ClpP and ClpX in the sar/agr regulatory network. Infect. Immun. 73:8100-8108.
11. Gaillot, O., E. Pellegrini, S. Bregenholt, S. Nair, and P. Berche. 2000. The ClpP serine protease is essential for the intracellular parasitism and virulence of Listeria monocytogenes. Mol. Microbiol. 35:1286-1294.
12. Gerth, U., E. Kruger, I. Derré, T. Msadek, and M. Hecker. 1998. Stress induction of the Bacillus subtilis clpP gene encoding a homologue of the proteolytic component of the Clp protease and the involvement of ClpP and ClpX in stress tolerance. Mol. Microbiol. 28:787-802.
13. Gottesman, S. 1999. Regulation by proteolysis: developmental switches. Curr. Opin. Microbiol. 2:142-147.
14. Hoskins, J., W. E. Alborn, Jr., J. Arnold, L. C. Blaszczak, S. Burgett, B. S. DeHoff, S. T. Estrem, L. Fritz, D. J. Fu, W. Fuller, C. Geringer, R. Gilmour, J. S. Glass, H. Khoja, A. R. Kraft, R. E. Lagace, D. J. LeBlanc, L. N. Lee, E. J. Lefkowitz, J. Lu, P. Matsushima, S. M. McAhren, M. McHenney, K. McLeaster, C. W. Mundy, T. I. Nicas, F. H. Norris, M. O'Gara, R. B. Peery, G. T. Robertson, P. Rockey, P. M. Sun, M. E. Winkler, Y. Yang, M. Young-Bellido, G. Zhao, C. A. Zook, R. H. Baltz, S. R. Jaskunas, P. R. Rosteck, Jr., P. L. Skatrud, and J. I. Glass. 2001. Genome of the bacterium Streptococcus pneumoniae strain R6. J. Bacteriol. 183:5709-5717.
15. Jenal, U., and T. Fuchs. 1998. An essential protease involved in bacterial cell-cycle control. EMBO J. 17:5658-5669.
16. Jones, J. M., D. J. Welty, and H. Nakai. 1998. Versatile action of Escherichia coli ClpXP as protease or molecular chaperone for bacteriophage Mu transposition. J. Biol. Chem. 273:459-465.
17. Kent, J. L., and R. D. Hotchkiss. 1964. Kinetic analysis of multiple, linked recombinations in pneumococcal transformation. J. Mol. Biol. 9:308-322.
18. Kirstein, J., D. Zühlke, U. Gerth, K. Turgay, and M. Hecker. 2005. A tyrosine kinase and its activator control the activity of the CtsR heat shock repressor in B. subtilis. EMBO J. 24:3435-3445.
19. Lee, M. S., and D. A. Morrison. 1999. Identification of a new regulator in Streptococcus pneumoniae linking quorum sensing to competence for genetic transformation. J. Bacteriol. 181:5004-5016.
20. Lemos, J. A., and R. A. Burne. 2002. Regulation and physiological significance of ClpC and ClpP in Streptococcus mutans. J. Bacteriol. 184:6357-6366.
21. Levchenko, I., L. Luo, and T. A. Baker. 1995. Disassembly of the Mu transposase tetramer by the ClpX chaperone. Genes Dev. 9:2399-2408.
22. Liu, J., W. M. Cosby, and P. Zuber. 1999. Role of Lon and ClpX in the post-translational regulation of a sigma subunit of RNA polymerase required for cellular differentiation in Bacillus subtilis. Mol. Microbiol. 33:415-428.
23. Luo, P. 2003. Genetic transformation in Streptococcus pneumoniae: regulation by ComX an alternative sigma factor. Ph.D. thesis. University of Illinois - Chicago, Chicago, IL.
24. Marmur, J. 1961. A procedure for the isolation of DNA from microorganisms. J. Mol. Biol. 3:208-218.
25. Martin, B., P. Garcia, M. P. Castanie, and J. P. Claverys. 1995. The recA gene of Streptococcus pneumoniae is part of a competence-induced operon and controls lysogenic induction. Mol. Microbiol. 15:367-379.
26. Maurizi, M. R. 1992. Proteases and protein degradation in Escherichia coli. Experientia 48:178-201.
27. Mhammedi-Alaoui, A., M. Pato, M. J. Gama, and A. Toussaint. 1994. A new component of bacteriophage Mu replicative transposition machinery: the Escherichia coli ClpX protein. Mol. Microbiol. 11:1109-1116.
28. Msadek, T., V. Dartois, F. Kunst, M. L. Herbaud, F. Denizot, and G. Rapoport. 1998. ClpP of Bacillus subtilis is required for competence development, motility, degradative enzyme synthesis, growth at high temperature and sporulation. Mol. Microbiol. 27:899-914.
29. Nair, S., C. Poyart, J. L. Beretti, H. Veiga-Fernandes, P. Berche, and P. Trieu-Cuot. 2003. Role of the Streptococcus agalactiae ClpP serine protease in heat-induced stress defence and growth arrest. Microbiology 149:407-417.
30. Nakano, S., M. M. Nakano, Y., Zhang, M. Leelakriangsak, and P. Zuber. 2003. A regulatory protein that interferes with activator-stimulated transcription in bacteria. Proc. Natl. Acad. Sci. USA 100:4233-4238.
31. Oggioni, M. R., and J.-P. Claverys. 1999. Repeated extra-genic sequences in prokaryotic genomes: a proposal for the origin and dynamics of the RUP element in Streptococcus pneumoniae. Microbiology 145:2647-2653.
32. Oruga, T., and A. J. Wilkinson. 2001. AAA+ superfamily ATPases: common structure - diverse function. Genes Cells 6:575-597.
33. Pestova, E. V., L. S. Havarstein, and D. A. Morrison. 1996. Regulation of competence for genetic transformation in Streptococcus pneumoniae by an auto-induced peptide pheromone and a two-component regulatory system. Mol. Microbiol. 21:853-862.
34. Piotrowski, A., P. Luo, and D. A. Morrison. 2009. Competence for genetic transformation in Streptococcus pneumoniae: termination of activity of the alternative sigma factor ComX is independent of the proteolysis of ComX and ComW. J. Bacteriol. 191:3359-3366.
35. Robertson, G. T., W. L. Ng, J. Foley, R. Gilmour, and M. E. Winkler. 2002. Global transcriptional analysis of clpP mutations of type 2 Streptococcus pneumoniae and their effects on physiology and virulence. J. Bacteriol. 184:3508-3520.
36. Robertson, G. T., W. L. Ng, R. Gilmour, and M. E. Winkler. 2003. Essentiality of clpX, but not clpP, clpL, clpC, or clpE, in Streptococcus pneumoniae R6. J. Bacteriol. 185:2961-2966.
37. Schelin, J., F. Lindmark, and A. K. Clarke. 2002. The clpP multigene family for the ATP-dependent Clp protease in the cyanobacterium Synechococcus. Microbiology 148:2255-2265.
38. Sung, C. K., H. Li, J.-P. Claverys, and D. A. Morrison. 2001. An rpsL cassette, Janus, for gene replacement through negative selection in Streptococcus pneumoniae. Appl. Environ. Microbiol. 67:5190-5196.
39. Sung, C. K., and D. A. Morrison. 2005. Two distinct functions of ComW in stabilization and activation of the alternative sigma factor ComX in Streptococcus pneumoniae. J. Bacteriol. 187:3052-3061.
40. Suzuki, C. K., M. Rep., J. M. van Dijl, K. Suda, L. A. Grivell, and G. Schatz. 1997. ATP-dependent proteases that also chaperone protein biogenesis. Trends Biochem. Sci. 22:118-123.
41. 32 in vivo. Mol. Microbiol. 30:583-593.
42. Tettelin, H., K. E. Nelson, I. T. Paulsen, J. A. Eisen, T. D. Read, S. Peterson, J. Heidelberg, R. T. DeBoy, D. H. Haft, R. J. Dodson, A. S. Durkin, M. Gwinn, J. F. Kolonay, W. C. Nelson, J. D. Peterson, L. A. Umayam, O. White, S. L. Salzberg, M. R. Lewis, D. Radune, E. Holtzapple, H. Khouri, A. M. Wolf, T. R. Utterback, C. L. Hansen, L. A. McDonald, T. V. Feldblyum, S. Angiuoli, T. Dickinson, E. K. Hickey, I. E. Holt, B. J. Loftus, F. Yang, H. O. Smith, J. C. Venter, B. A. Dougherty, D. A. Morrison, S. K. Hollingshead, and C. M. Fraser. 2001. Complete genome sequence of a virulent isolate of Streptococcus pneumoniae. Science 293:498-506.
43. Tomoyasu, T., T. Ohkishi, Y. Ukyo, A. Tokumitsu, A. Takaya, M. Suzuki, K. Sekiya, H. Matsui, K. Kutsukake, and T. Yamamoto. 2002. The ClpXP ATP-dependent protease regulates flagellum synthesis in Salmonella enterica serovar Typhimurium. J. Bacteriol. 184:645-653.
44. Turgay, K., J. Hahn, J. Burghoorn, and D. Dubnau. 1998. Competence in Bacillus subtilis is controlled by regulated proteolysis of a transcription factor. EMBO J. 17:6730-6738.
45. Viala, J., and P. Mazodier. 2003. The ATPase ClpX is conditionally involved in the morphological differentiation of Streptomyces lividans. Mol. Genet. Genomics 268:563-569.
46. Wawrzynow, A., D. Wojtkowiak, J. Marszalek, B. Banecki, M. Jonsen, B. Graves, C. Georgopolous, and M. Zylicz. 1995. The ClpX heat shock protein of Escherichia coli, the ATP-dependent substrate specificity component of the ClpP-ClpX protease, is a novel molecular chaperone. EMBO J. 14:1867-1877.
47. Weart, R. B., S. Nakano, B. E. Lane, P. Zuber, and P. A. Levin. 2005. The ClpX chaperone modulates assembly of the tubulin-like protein FtsZ. Mol. Microbiol. 57:238-249.
48. Zellmeier, S., W. Schumann, and T. Wiegert. 2006. Involvement of Clp protease activity in modulating the Bacillus subtilis sigma stress response. Mol. Microbiol. 61:1569-1582.
49. Zhou, Y., and S. Gottesman. 1998. Regulation of proteolysis of the stationary-phase sigma factor RpoS. J. Bacteriol. 180:1154-1158.