Purification and characterization of a ∼34 kDa antioxidant protein (β-turmerin) from turmeric (Curcuma longa) waste grits

Biochimie

Volumn 91, Issue 9, 2009, Pages 1156-1162

  Smitha, S ^a   Dhananjaya, B L ^a   Dinesha, R ^a   Srinivas, Leela ^a  

^a ADICHUNCHANAGIRI INSTITUTE OF MEDICAL SCIENCES   (India)

Author keywords

Turmerin; Antioxidant; Lipid peroxidation; Non toxic; Reactive oxygen species (ROS); Turmeric waste grits

Indexed keywords

ALKADIENE; ALPHA TOCOPHEROL; ANTIOXIDANT; BETA TURMERIN; BUTYLATED HYDROXYANISOLE; CURCUMIN; HYDROXYL RADICAL; LINOLENIC ACID; LIPOSOME; MANNITOL; REACTIVE OXYGEN METABOLITE; UNCLASSIFIED DRUG; BUTYLCRESOL; PEPTIDE; PLANT EXTRACT; TURMERIC EXTRACT; TURMERIN; VEGETABLE PROTEIN;

ANTIOXIDANT ACTIVITY; APOPTOSIS; ARTICLE; CELL VIABILITY; CONJUGATION; CONTROLLED STUDY; CURCUMA LONGA; DRUG EFFICACY; DRUG ISOLATION; DRUG MECHANISM; DRUG PURIFICATION; ERYTHROCYTE MEMBRANE; GEL PERMEATION CHROMATOGRAPHY; HUMAN; HUMAN CELL; LIPID PEROXIDATION; LYMPHOCYTE; MICELLE; MOLECULAR WEIGHT; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; CELL CULTURE; CELL DEATH; CHEMISTRY; DRUG EFFECT; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; ISOLATION AND PURIFICATION; METABOLISM; MONONUCLEAR CELL; SPECTROFLUOROMETRY;

CURCUMA LONGA;

ANTIOXIDANTS; BUTYLATED HYDROXYTOLUENE; CELL DEATH; CELLS, CULTURED; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CURCUMA; ELECTROPHORESIS, POLYACRYLAMIDE GEL; HUMANS; LEUKOCYTES, MONONUCLEAR; LIPID PEROXIDATION; LYMPHOCYTES; PEPTIDES; PLANT EXTRACTS; PLANT PROTEINS; REACTIVE OXYGEN SPECIES; SPECTROMETRY, FLUORESCENCE;

EID: 67749084101     PISSN: 03009084     EISSN: 61831638     Source Type: Journal    
DOI: 10.1016/j.biochi.2009.06.006     Document Type: Article

References (41)

1. Droge W. Free radicals in the physiological control of cell function. Physiol. Rev. 282 (2002) 47-95
2. Butterfield D.A., Koppal T., Howard B., Subramanium R., Hall N., Hensley K., Yatin S., Allen K., Aksenov M., Aksenova M., and Carney J. Structural and functional changes in proteins induced by free radical-mediated oxidative stress and protective action of the antioxidants N-tert-butyl-alpha-phenylnitrone and vitamin E. Ann. N.Y. Acad. Sci. 854 (1998) 448-462
3. Lee H.C., and Wei Y.H. Oxidative stress, mitochondrial DNA mutation, and apoptosis in aging. Exp. Biol. Med. (Maywood) 232 (2007) 592-606
4. Valko M., Leibfritz D., Moncol J., Cronin M.T., Mazur M., and Telser J. Free radicals and antioxidants in normal physiological functions and human disease. Int. J. Biochem. Cell Biol. 39 (2007) 44-84
5. Halliwell B. Free radicals, antioxidants and human diseases; curiosity, cause, or consequences?. Lancet 334 (1994) 721-724
6. Aviram M. Review of human studies on oxidative damage and antioxidant protection related to cardiovascular diseases. Free Radic. Res. 3 (2000) S85-S97
7. Kris-Etherton P.M., Lichtenstein A.H., Howard B.V., Steinberg D., and Wiztum J.L. Nutrition committee of the American heart association council on nutrition, physical activity and metabolism. Antioxidant vitamin supplements and cardiovascular disease. Circulation 110 (2004) 637-641
8. Riccioni G., Mancini B., DiIIio E., Bucciarelli T., and `Orazio N.D. Protective effect of lycopene in cardiovascular disease. Eur. Rev. Med. Pharmacol. Sci. 12 (2008) 183-190
9. Watts P. BHA isomers and hyperplasia. Food Chem. Toxicol. 23 (1985) 639-640
10. Kaur C., and Kapoor H.C. Antioxidants in fruits and vegetables - the millennium`s health. Int. J. Food Sci. Technol. 36 (2001) 703-725
11. Seifried H.E., McDonald S.S., Anderson D.E., Greenwald P., and Milner J.A. The antioxidant conundrum in cancer. Cancer Res. 63 (2003) 4295-4298
12. Clarke M.W., Burnett J.R., and Croft K.D. Vitamin E in human health and disease. Crit. Rev. Clin. Lab. Sci. 45 (2008) 417-450
13. Ammon H.P.T., and Wahl M.A. Pharmacology of Curcuma longa. Planta Med. 57 (1991) 1-7
14. Ishita C., Kaushik B., Uday B., and Ranajit K.B. Turmeric and curcumin: biological actions and medicinal applications. Curr. Sci. 87 (2004) 44-53
15. Srinivas L., Shalini V.K., and Shylaja M. Turmerin: a water-soluble antioxidant peptide from turmeric (Curcuma longa. Arch. Biochem. Biophys. 292 (1992) 617-623
16. 2 by turmerin, a protein from Curcuma longa L. Biol. Chem. 389 (2008) 299-303
17. Sivapriya M., and Srinivas L. Isolation and purification of a novel antioxidant protein from the water extract of sundakai (Solanum torvum) seeds. Food Chem. 104 (2007) 510-517
18. Ningappa M.B., and Srinivas L. Purification and characterization of approximately 35 kDa antioxidant protein from curry leaves (Murraya koenigii L.). Toxicol. in Vitro 22 (2008) 699-709
19. Cohly H.H.P., Asad S., Das S.K., Angel M.F., and Rao M. Effect of antioxidant (turmeric, turmerin and curcumin) on human immunodeficiency virus. Int. J. Mol. Sci. 4 (2003) 22-33
20. Laemmli U.K. Cleavage of structural proteins during the assembly of head of bacteriophage T4. Nature 227 (1970) 680-685
21. Davis B.J. Disk electrophoresis, II. Method and application of human serum proteins. Ann. N.Y. Acad. Sci. 121 (1964) 404-427
22. Bradford M.M. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein dye binding. Anal. Biochem. 7 (1976) 248-254
23. Sadasivam S., and Manickam A. Identification of Proteins, Biochemical Methods. second ed. (1997), New Age International Publishers pp. 33
24. Ellman G.L. Tissue sulfhydryl groups. Arch. Biochem. Biophys. 82 (1959) 70-77
25. Folch J., Less M., and Sloane-Stanley G.H. A simple method for the isolation and purification of total lipids from animal tissues. J. Biol. Chem. 226 (1957) 497-509
26. Dubois M., Gilles K.A., Hamilton J.K., Rebers P.A., and Smith F. Colorimetric method for determination of sugar and related substances. Anal. Chem. 28 (1956) 899-910
27. Kujala T.S., oponen J.M., Kloka K.D., and Pihlaja K. Phenolics and betacyanins in red beetroot (Beta Vulgaris) root: distribution and effect of cold storage on the contents of total phenolics and three individual compounds. J. Agric. Food Chem. 48 (2000) 5338-5342
28. Ravindranath V., Satyanarayanan M.N., and Rao M.V.L. Rubrocurcumin reaction and its use in micro determination of certain organic acids. Indian J. Chem. 20 (1981) 907-909
29. Shimazaki H., Ueta N., Mowri H.O., and Inoue K. Formation of age pigment like fluorescent substances during peroxidation of lipids in model membranes. Biochim. Biophys. Acta 792 (1984) 123-128
30. Dahle L.K., Hill E.G., and Holman R.T. The thiobarbituric acid reaction and the autooxidants of polyunsaturated fatty acid methyl esters. Arch. Biochem. Biophys. 98 (1962) 253-261
31. Dodge J.T., Mitchell C., and Hanahan D.J. The preparation and chemical characteristics of hemoglobin free ghosts of human erythrocytes. Arch. Biochem. Biophys. 100 (1963) 119-128
32. Fenton H.J.H. Oxidation of tartaric acid in the presence of iron. J. Chem. Soc. 106 (1984) 899-910
33. Halliwell B., Gutteridge J.M.C., and Aruoma O.I. The deoxyribose method: a simple assay for determination of rate constants for reactions of hydroxyl radicals. Anal. Biochem. 165 (1987) 215-219
34. Stoffel W., and Ahrens E.H. Isolation and structure of the C16 unsaturated fatty acids in menhaden body oil. J. Am. Chem. Soc. 80 (1958) 6604-6608
35. 2-production by human neutrophils. The preparation and assay of NADPH oxidase-containing particles from human neutrophils. Methods Enzymol. 105 (1984) 358-365
36. Phillips H.J. Dye exclusion tests for cell viability. In: Kruse P.F., and Patterson M.K. (Eds). Tissue Culture Methods (1973) 406-408
37. Patterson C.E., and Rhoades R.A. Protective role of sulfhydryl reagents in oxidant lung injury. Exp. Lung Res. 14 (1988) 1005-1019
38. Selvam R., and Devaraj S. Oxalate binding to rat kidney mitochondria: induction by oxidized glutathione. Indian J. Biochem. Biophys. 33 (1996) 62-65
39. Buege J.A., and Aust S.D. Microsomal lipid peroxidation. Methods. Enzymol. 52 (1978) 302-310
40. Rice-Evans C., and Burdon R. Free radical-lipid interactions and their pathological consequences. Prog. Lipid Res. 32 (1993) 71-110
41. Held K.D., Sylvester F.C., Hopica K.L., and Biaglow J.E. Role of Fenton chemistry in thiol-induced toxicity and apoptosis. Radiat. Res. 145 (1996) 542-553