Simultaneous optical mapping of intracellular free calcium and action potentials from langendorff perfused hearts

Current Protocols in Cytometry

Volumn , Issue SUPPL. 49, 2009, Pages

  Salama, Guy ^a   Hwang, Seong Min ^a  

^a UNIVERSITY OF PITTSBURGH   (United States)

Author keywords

Action potential (AP); Cai indicator; Complementary metal oxide silicon (CMOS) camera; Intracellular free Ca2+ (Cai); Photodiode array (PDA); Pittsburgh I (PGH I); RH237; Rhod 2AM; Voltage Sensitive Dye (VSD)

Indexed keywords

FLUORESCENT DYE;

CALCIUM CELL LEVEL; CALCIUM MOBILIZATION; CARDIOMYOPATHY; CHEMICAL LABELING; EXCITATION CONTRACTION COUPLING; FLUORESCENCE ANALYSIS; HEART ARRHYTHMIA; HEART FAILURE; HEART INFARCTION; HEART MUSCLE FIBER MEMBRANE POTENTIAL; HEART MUSCLE ISCHEMIA; HEART MUSCLE POTENTIAL; HEART VENTRICLE FIBRILLATION; ION CURRENT; ISOLATED HEART; LONG QT SYNDROME; MOLECULAR SENSOR; OPTICAL INSTRUMENTATION; PRIORITY JOURNAL; REVIEW; SIGNAL PROCESSING;

ACTION POTENTIALS; ANIMALS; ARRHYTHMIAS, CARDIAC; CALCIUM; COLORING AGENTS; CYTOPHOTOMETRY; HEART; HEART FAILURE; HUMANS; KINETICS; MICE; MICROSCOPY, FLUORESCENCE; PERFUSION; RATS; TIME FACTORS;

EID: 67651113442     PISSN: 19349297     EISSN: 19349300     Source Type: Journal    
DOI: 10.1002/0471142956.cy1217s49     Document Type: Review

References (92)

1. 2+ under various conditions. J. Mol. Cell. Cardiol. 24:765-773.
2. Allingham, J.S., Smith, R., and Rayment, I. 2005. The structural basis of blebbistatin inhibition and specificity for myosin II. Nat. Struct. Mol. Biol. 12:378-379.
3. 2+ network. J. Cell. Biol. 136:833-844.
4. Baker, L.C.,Wolk, R.,Choi,B.R.,Watkins, S., Plan, P., Shah, A., and Salama, G. 2004. Effects of mechanical uncouplers, diacetyl monoxime, and cytochalasin-D on the electrophysiology of perfused mouse hearts. Am. J. Physiol. Heart Circ. Physiol. 287:H1771-H1779.
5. Bassani, J.W., Bassani, R.A., and Bers, D.M. 1995. Calibration of indo-1 and resting intracellular [Ca]i in intact rabbit cardiac myocytes. Biophys. J. 68:1453-1460.
6. Biermann, M., Rubart, M., Moreno, A., Wu, J., Josiah-Durant, A., and Zipes, D.P. 1998. Differential effects of cytochalasin D and 2,3 butanedione monoxime on isometric twitch force and transmembrane action potential in isolated ventricular muscle: Implications for optical measurements of cardiac repolarization. J. Cardiovasc. Electrophysiol. 9:1348-1357.
7. Blatter, L.A. and Wier, W.G. 1990. Intracellular diffusion, binding, and compartmentalization of the fluorescent calcium indicators indo-1 and fura-2. Biophys. J. 58:1491-1499.
8. Brown, N.H., Dobrovolny, H.M., Gauthier, D.J., and Wolf, P.D. 2007. A fiber-based ratiometric optical cardiac mapping channel using a diffraction grating and split detector. Biophys. J. 93:254-263.
9. Chacon, E., Ohata, H., Harper, I.S., Trollinger, D.R., Herman, B., and Lemasters, J.J. 1996. Mitochondrial free calcium transients during excitation-contraction coupling in rabbit cardiac myocytes. FEBS Lett. 382:31-36.
10. Cheng, Y., Li, L., Nikolski, V., Wallick, D.W., and Efimov, I.R. 2004. Shock-induced arrhythmogenesis is enhanced by 2,3-butanedione monoxime compared with cytochalasin D. Am. J. Physiol. Heart Circ. Physiol. 286:H310-H318.
11. Choi, B.R. and Salama, G. 2000. Simultaneous maps of optical action potentials and calcium transients in guinea-pig hearts: Mechanisms underlying concordant alternans. J. Physiol. 529:171-188.
12. 2+ triggers early afterdepolarizations and Torsade de Pointes in rabbit hearts with type 2 long QT syndrome. J. Physiol. 543:615-631.
13. Choi, B.R., Hatton, W.J., Hume, J.R., Liu, T., and Salama, G. 2006a. Low osmolarity transforms ventricular fibrillation from complex to highly organized, with a dominant high-frequency source. Heart Rhythm 3:1210-1220.
14. Choi, B.R., Liu, T., and Salama, G. 2006b. Calcium transients modulate action potential repolarizations in ventricular fibrillation. Conf. Proc. IEEE Eng. Med. Biol. Soc. 1:2264-2267.
15. Choi,B.R., Jang,W., and Salama,G. 2007. Spatially discordant voltage alternans cause wavebreaks in ventricular fibrillation. Heart Rhythm 4:1057-1068.
16. Chou, C.C., Nguyen, B.L., Tan, A.Y., Chang, P.C., Lee, H.L., Lin, F.C., Yeh, S.J., Fishbein, M.C., Lin, S.F., Wu, D., Wen, M.S., and Chen, P.S. 2008. Intracellular calcium dynamics and acetylcholine-induced triggered activity in the pulmonary veins of dogs with pacing-induced heart failure. Heart Rhythm 5:1170-1177.
17. Cohen, L. 1995. Optical monitoring of activity in the nervous system: A brief history. Tsitologiia 37:1136-1141.
18. 2+ and the heart. Cell Calcium 44:77-91.
19. Del Nido, P.J., Glynn, P., Buenaventura, P., Salama, G., and Koretsky, A.P. 1998. Fluorescence measurement of calcium transients in perfused rabbit heart using rhod 2. Am. J. Physiol. 274:H728-H741.
20. Djurisic,M., Zochowski, M.,Wachowiak, M., Falk, C.X., Cohen, L.B., and Zecevic, D. 2003. Optical monitoring of neural activity using voltagesensitive dyes. Methods Enzymol. 361:423-451.
21. Dou, Y., Arlock, P., and Arner, A. 2007. Blebbistatin specifically inhibits actin-myosin interaction in mouse cardiac muscle. Am. J. Physiol. Cell. Physiol. 293:C1148-C1153.
22. Duchen, M.R., Leyssens, A., Mojet, M.H., and Peuchen, S. 1996. Properties of rhod 2 as an indicator of intra-mitochondrial calcium (abstract). J. Physiol. 494:9P.
23. Efimov, I.R.,Huang, D.T., Rendt, J.M., and Salama, G. 1994. Optical mapping of repolarization and refractoriness from intact hearts. Circulation 90:1469-1480.
24. Efimov, I.R., Nikolski, V.P., and Salama, G. 2004. Optical imaging of the heart. Circ. Res. 95:21-33.
25. Entcheva, E. and Bien, H. 2006. Macroscopic optical mapping of excitation in cardiac cell networkswith ultra-high spatiotemporal resolution. Prog. Biophys. Mol. Biol. 92:232-257.
26. Fast, V.G. 2005. Simultaneous optical imaging of membrane potential and intracellular calcium. J. Electrocardiol. 38:107-112.
27. Fast, V.G. and Ideker, R.E. 2000. Simultaneous optical mapping of transmembrane potential and intracellular calcium in myocyte cultures. J. Cardiovasc. Electrophysiol. 11:547-556.
28. Fedorov, V.V., Lozinsky, I.T., Sosunov, E.A., Anyukhovsky, E.P., Rosen, M.R., Balke, C.W., and Efimov, I.R. 2007. Application of blebbistatin as an excitation-contraction uncoupler for electrophysiologic study of rat and rabbit hearts. Heart Rhythm 4:619-626.
29. Fossum, E.R. 1993a. Active-pixel sensors challenge CCDs. Laser Focus World 29:83-87.
30. Fossum, E.R. 1993b. Active pixel sensors: Are CCDs dinosaurs? Proc. SPIE 1900:2-14.
31. + channel to the actin cytoskeleton. Pflugers Arch. 431:504-512.
32. Gwathmey, J.K., Hajjar, R.J., and Solaro, R.J. 1991. Contractile deactivation and uncoupling of crossbridges: Effects of 2,3-butanedione monoxime on mammalian myocardium. Circ. Res. 69:1280-1292.
33. Hajnoczky, G., Robb-Gaspers, L.D., Seitz, M.B., and Thomas, A.P. 1995. Decoding of cytosolic calcium oscillations in the mitochondria. Cell 82:415-424.
34. Haworth, R.A. and Redon, D. 1998. Calibration of intracellular Ca transients of isolated adult heart cells labelledwith fura-2 by acetoxymethyl ester loading. Cell Calcium 24:263-273.
35. Hayashi, H., Kamanu, S.D., Ono, N., Kawase, A., Chou, C.C., Weiss, J.N., Karagueuzian, H.S., Lin, S.F., and Chen, P.S. 2008. Calcium transient dynamics and the mechanisms of ventricular vulnerability to single premature electrical stimulation in Langendorff-perfused rabbit ventricles. Heart Rhythm 5:116-123.
36. Hove-Madsen, L. and Bers, D.M. 1992. Indo-1 binding to protein in permeabilized ventricular myocytes alters its spectral and Ca binding properties. Biophys. J. 63:89-97.
37. Inagaki, M., Hidaka, I., Aiba, T., Tatewaki, T., Sunagawa, K., and Sugimachi, M. 2004. High resolution optical mapping of cardiac action potentials in freely beating rabbit hearts. Conf. Proc. IEEE Eng. Med. Biol. Soc. 5:3578-3580.
38. Jalife, J., Morley, G.E., Tallini, N.Y., and Vaidya, D. 1998. A fungal metabolite that eliminates motion artifacts. J. Cardiovasc. Electrophysiol. 9:1358-1362.
39. 2+ oscillations in single living cells revealed by rhod-2 and laser confocal microscopy. Biophys. J. 66:A94.
40. 2+ concentration in single cultured rat brain astrocytes. J. Physiol. 497:299-308.
41. Katra, R.P. and Laurita, K.R. 2005. Cellular mechanism of calcium-mediated triggered activity in the heart. Circ. Res. 96:535-542.
42. Katra, R.P., Pruvot, E., and Laurita, K.R. 2004. Intracellular calcium handling heterogeneities in intact guinea pig hearts. Am. J. Physiol. Heart Circ. Physiol. 286:H648-H656.
43. Kettlewell, S., Walker, N.L., Cobbe, S.M., Burton, F.L., and Smith, G.L. 2004. The electrophysiological and mechanical effects of 2,3-butanedione monoxime and cytochalasin-D in the Langendorff perfused rabbit heart. Exp. Physiol. 89:163-172.
44. Knisley, S.B., Justice, R.K., Kong, W., and Johnson, P.L. 2000. Ratiometry of transmembrane voltage-sensitive fluorescent dye emission in hearts. Am. J. Physiol. Heart Circ. Physiol. 279:H1421-H1433.
45. Knisley, S.B. and Pollard, A.E. 2005. Use of translucent indium tin oxide to measure stimulatory effects of a passive conductor during field stimulation of rabbit hearts. Am. J. Physiol. Heart Circ. Physiol. 289:H1137-H1146.
46. Kong, W., Walcott, G.P., Smith, W.M., Johnson, P.L., and Knisley, S.B. 2003. Emission ratiometry for simultaneous calcium and action potential measurements with coloaded dyes in rabbit hearts: Reduction of motion and drift. J. Cardiovasc. Electrophysiol. 14:76-82.
47. Lakireddy, V., Baweja, P., Syed, A., Bub, G., Boutjdir, M., and El-Sherif, N. 2005. Contrasting effects of ischemia on the kinetics of membrane voltage and intracellular calcium transient underlie electrical alternans. Am. J. Physiol. Heart Circ. Physiol. 288:H400-H407.
48. Lakireddy, V., Bub, G., Baweja, P, Syed, A, Boutjdir, M, and El-Sherif, N. 2006. The kinetics of spontaneous calcium oscillations and arrhythmogenesis in the in vivo heart during ischemia/reperfusion. Heart Rhythm 3:58- 66.
49. Lan, D.Z., Pollard, A.E., Knisley, S.B., and Fast, V.G. 2007. Optical mapping of V(m) and Ca(i)(2+) in a model of arrhythmias induced by local catecholamine application in patterned cell cultures. Pflugers Arch. 453:871-877.
50. Laude, B., de Martino, A., Drévillon, B., Benattar, L., and Schwartz, L. 2002. Full-field optical coherence tomography with thermal light. App. Optics 41:6637-6645.
51. Laurita, K.R. and Rosenbaum, D.S. 2008. Mechanisms and potential therapeutic targets for ventricular arrhythmias associated with impaired cardiac calcium cycling. J. Mol. Cell. Cardiol. 44:31-43.
52. Laurita, K.R. and Singal, A. 2001. Mapping action potentials and calcium transients simultaneously from the intact heart. Am. J. Physiol. Heart Circ. Physiol. 280:H2053-H2060.
53. Li, D. and Nattel, S. 2007. Pharmacological elimination of motion artifacts during optical imaging of cardiac tissues: Is blebbistatin the answer? Heart Rhythm 4:627-628.
54. Li, T., Sperelakis, N., Teneick, R.E., and Solaro, R.J. 1985. Effects of diacetyl monoxime on cardiac excitation-contraction coupling. J. Pharmacol. Exp. Ther. 232:688-695.
55. Liu, Y., Cabo, C., Salomonsz, R., Delmar, M., Davidenko, J., and Jalife, J. 1993. Effects of diacetyl monoxime on the electrical properties of sheep and guinea pig ventricular muscle. Cardiovasc. Res. 27:1991-1997.
56. 2+ transients evoke concurrent mitochondrial depolarizations. Proc. Natl. Acad. Sci.U.S.A. 91:12579-12583.
57. Loew, L.M., Tuft, R.A., Carrington, W., and Fay, F.S. 1993. Imaging in five dimensions: Timedependent membrane potentials in individual mitochondria. Biophys. J. 65:2396-2407.
58. London, B., Baker, L.C., Lee, J.S., Shusterman, V., Choi, B.R., Kubota, T., McTiernan, C.F., Feldman, A.M., and Salama, G. 2003. Calciumdependent arrhythmias in transgenic mice with heart failure. Am. J. Physiol. Heart Circ. Physiol. 284:H431-H441.
59. London, B., Baker, L.C., Petkova-Kirova, P., Nerbonne, J.M., Choi, B.R., and Salama, G. 2007. Dispersion of repolarization and refractoriness are determinants of arrhythmia phenotype in transgenic mice with long QT. J. Physiol. 578:115-129.
60. Lorell, B.H., Apstein, C.S., Cunningham, M.J., Schoen, F.J.,Weinberg, E.O., Peeters, G.A., and Barry, W.H. 1990. Contribution of endothelial cells to calcium-dependent fluorescence transients in rabbit hearts loaded with indo 1. Circ. Res. 67:415-425.
61. 2+ wave characteristics. Cell Calcium 34:1-9.
62. + channels. FASEB J. 10:357-361.
63. 2+ and probenecid. Cell Calcium 10:171-180.
64. Mendis, S., Kemeny, S.E., and Fossum, E.R. 1994. CMOS active pixel image sensor. IEEE Trans. Electron. Devices 41:452-453.
65. Mendis, S.K., Pain, B., Nixon, R.H., and Fossum, E.R. 1993. Low-light-level image sensor with on-chip signal processing. Proc. SPIE 1952:23-33.
66. Minta, A., Kao, J.P., and Tsien, R.Y. 1989. Fluorescent indicators for cytosolic calcium based on rhodamine and fluorescein chromophores. J. Biol. Chem. 264:8171-8178.
67. Mitra, R. and Morad, M. 1985. A uniform enzymatic method for dissociation of myocytes from hearts and stomachs of vertebrates. Am. J. Physiol. 249:H1056-H1060.
68. 2+ following stimulation of cell contraction. Biophys. J. 66:A97.
69. 2+ concentration in living single rat cardiac myocytes. Am. J. Physiol. 261:H1123-H1134.
70. 2+ release from cardiac sarcoplasmic reticulum. Arch. Biochem. Biophys. 282:275-283.
71. Roell, W., Lewalter, T., Sasse, P., Tallini, Y.N., Choi, B.R., Breitbach, M., Doran, R., Becher, U.M., Hwang, S.M., Bostani, T., von Maltzahn, J., Hofmann, A., Reining, S., Eiberger, B., Gabris, B., Pfeifer, A., Welz, A., Willecke, K., Salama, G., Schrickel, J.W., Kotlikoff, M.I., and Fleischmann, B.K. 2007. Engraftment of connexin 43-expressing cells prevents post-infarct arrhythmia. Nature 450:819-824.
72. 2+ with recombinant targeted aequorin: Significance for the regulation of pyruvate dehydrogenase activity. Proc. Natl. Acad. Sci. U.S.A. 93:5489-5494.
73. Saba, S., Mathier, M.A., Mehdi, H., Liu, T., Choi, B.R., London, B., and Salama, G. 2008. Dualdye optical mapping after myocardial infarction: Does the site of ventricular stimulation alter the properties of electrical propagation? J. Cardiovasc. Electrophysiol. 19:197-202.
74. 2+ cycling? Heart Rhythm 31:67-70.
75. Salama, G. and Choi, B.R. 2007. Imaging ventricular fibrillation. J. Electrocardiol. 40:S56-S61.
76. Salama, G., Choi, B.R., Azour, G., Lavasani, M., Tumbev,V., Salzberg, B.M., Patrick,M.J., Ernst, L.A., and Waggoner, A.S. 2005. Properties of new, long-wavelength, voltage-sensitive dyes in the heart. J. Membr. Biol. 208:125-140.
77. Salama, G., Lombardi, R., and Elson, J. 1987. Maps of optical action potentials and NADH fluorescence in intact working hearts. Am. J. Physiol. 252:H384-H394.
78. Salama, G. and Morad, M. 1976. Merocyanine 540 as an optical probe of transmembrane electrical activity in the heart. Science 191:485-487.
79. 2+ measurement. J. Biol. Chem. 262:6308-6312.
80. 2+. Biophys. J. 70:2571-2580.
81. Sellin, L.C. and McArdle, J.J. 1994. Multiple effects of 2,3-butanedione monoxime. Pharmacol. Toxicol. 74:305-313.
82. Straight, A.F., Cheung, A., Limouze, J., Chen, I., Westwood, N.J., Sellers, J.R., and Mitchison, T.J. 2003. Dissecting temporal and spatial control of cytokinesis with a myosin II inhibitor. Science 299:1743-1747.
83. 2+ waves and arteriolar dilation in vivo: Measurements in Cx40BAC GCaMP2 transgenic mice. Circ. Res. 101:1300-1309.
84. 2+ indicator GCaMP2. Proc.Natl. Acad. Sci.U.S.A. 103:4753-4758.
85. Tsien, R.Y. 1980. New calcium indicators and buffers with high selectivity against magnesium and protons: Design, synthesis, and properties of prototype structures. Biochemistry 19:2396-2404.
86. Undrovinas, A.I., Shander, G.S., and Makielski, J.C. 1995. Cytoskeleton modulates gating of voltage-dependent sodium channel in heart. Am. J. Physiol. 269:H203-H214.
87. 2+ concentrations. Cell Calcium 12:29-37.
88. Wu, J., Biermann, M., Rubart, M., and Zipes, D.P. 1998. Cytochalasin D as excitation-contraction uncoupler for optically mapping action potentials in wedges of ventricular myocardium. J. Cardiovasc. Electrophysiol. 9:1336-1347.
89. Xie, L.-H., Chen, F., Karagueuzian, H.S., and Weiss, J.N. 2009. Oxidative stress-induced afterdepolarizations and calmodulin kinase II signaling. Circ. Res. 104:79-86.
90. 2+ release from sarcoplasmic reticulum via an oxidation reaction. J. Biol. Chem. 264:21725-21736.
91. Zecevic, D., Djurisic, M., Cohen, L.B., Antic, S., Wachowiak, M., Falk, C.X., and Zochowski, M.R. 2003. Imaging nervous system activity with voltage-sensitive dyes. Curr. Protoc. Neurosci. 23:6.17.1-6.17.29.
92. Zimmermann, H. 2000. Integrated Silicon Optoelectronics. Springer-Verlag, Berlin.