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Volumn 79, Issue 6, 2009, Pages

DNA electrophoresis in a sparse ordered post array

Author keywords

[No Author keywords available]

Indexed keywords

BROWNIAN DYNAMICS; COIL SIZES; DISPERSIVITY; DNA TRANSPORT; ELECTRIC FIELD GRADIENTS; HEXAGONAL ARRAYS; MICRO-FLUIDIC DEVICES; MICRO-SCALE; NONUNIFORM ELECTRIC FIELD; STRONG ELECTRIC FIELDS; UNIFORM ELECTRIC FIELDS; VIDEOMICROSCOPY;

EID: 67650886441     PISSN: 15393755     EISSN: 15502376     Source Type: Journal    
DOI: 10.1103/PhysRevE.79.061904     Document Type: Article
Times cited : (34)

References (34)
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  • 5
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    • 10.1103/PhysRevLett.93.058102
    • G. C. Randall and P. S. Doyle, Phys. Rev. Lett. 93, 058102 (2004). 10.1103/PhysRevLett.93.058102
    • (2004) Phys. Rev. Lett. , vol.93 , pp. 058102
    • Randall, G.C.1    Doyle, P.S.2
  • 12
    • 85036352478 scopus 로고    scopus 로고
    • 10.1103/PhysRevE.69.011901;
    • K. D. Dorfman and J.-L. Viovy, Phys. Rev. E 69, 011901 (2004) 10.1103/PhysRevE.69.011901
    • (2004) Phys. Rev. e , vol.69 , pp. 011901
    • Dorfman, K.D.1    Viovy, J.-L.2
  • 15
    • 35548945443 scopus 로고    scopus 로고
    • 10.1103/PhysRevE.76.040903
    • A. Mohan and P. S. Doyle, Phys. Rev. E 76, 040903 (R) (2007). 10.1103/PhysRevE.76.040903
    • (2007) Phys. Rev. e , vol.76 , pp. 040903
    • Mohan, A.1    Doyle, P.S.2
  • 18
    • 67650785609 scopus 로고    scopus 로고
    • The post array was molded from a photoresist mold (Shipley S1818) patterned by photolithography. To assemble the chips, we spin-coated a #1 coverslip with a thin film of PDMS and bonded it to the replica-molded PDMS post array after exposing both substrates to an oxygen plasma. A 1″ × 3″ microscope slide with large cylindrical reservoirs (ID=7 mm) was bonded to the back side of the chip for reinforcement and to suppress hydrodynamic flow. The completed and sealed chips were filled with TBE 2.2× buffer and submerged in buffer for 48 h at 55°C, a modification of an existing protocol for preventing pervaporation. To perform replica experiments, the chips were regenerated by emptying them, refilling with neat buffer, and then soaking again in buffer at 55°C.
    • The post array was molded from a photoresist mold (Shipley S1818) patterned by photolithography. To assemble the chips, we spin-coated a #1 coverslip with a thin film of PDMS and bonded it to the replica-molded PDMS post array after exposing both substrates to an oxygen plasma. A 1″ × 3″ microscope slide with large cylindrical reservoirs (ID=7 mm) was bonded to the back side of the chip for reinforcement and to suppress hydrodynamic flow. The completed and sealed chips were filled with TBE 2.2× buffer and submerged in buffer for 48 h at 55°C, a modification of an existing protocol for preventing pervaporation. To perform replica experiments, the chips were regenerated by emptying them, refilling with neat buffer, and then soaking again in buffer at 55°C.
  • 23
    • 67650842952 scopus 로고    scopus 로고
    • The electric field in the separation arm was computed from the applied potentials using Kirchoff's laws and assuming a uniform resistivity.
    • The electric field in the separation arm was computed from the applied potentials using Kirchoff's laws and assuming a uniform resistivity.
  • 24
    • 67650830007 scopus 로고    scopus 로고
    • Our measurement of μ0 =1.8× 10-4 cm2 /Vs agrees well with previous measurements in PDMS devices.
    • Our measurement of μ0 =1.8× 10-4 cm2 /Vs agrees well with previous measurements in PDMS devices.
  • 33
    • 33747593555 scopus 로고    scopus 로고
    • 10.1063/1.2222374
    • J. M. Kim and P. S. Doyle, J. Chem. Phys. 125, 074906 (2006). 10.1063/1.2222374
    • (2006) J. Chem. Phys. , vol.125 , pp. 074906
    • Kim, J.M.1    Doyle, P.S.2


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.