Rapid multiplexed flow cytometric assay for botulinum neurotoxin detection using an automated fluidic microbead-trapping flow cell for enhanced sensitivity

Analytical Chemistry

Volumn 81, Issue 14, 2009, Pages 5783-5793

  Ozanich Jr , Richard M ^a   Bruckner Lea, Cynthia J ^a   Warner, Marvin G ^a   Miller, Keith ^a   Antolick, Kathryn C ^a   Marks, James D ^b   Lou, Jianlong ^b   Grate, Jay W ^a  

^a PACIFIC NORTHWEST NATIONAL LABORATORY   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AUTOMATED FLUIDICS; BOTULINUM NEUROTOXINS; ENHANCED SENSITIVITY; FLOW CELLS; FLOW-CYTOMETER; FLUIDIC SYSTEMS; FLUORESCENCE INTENSITIES; FLUORESCENT DYES; HEAVY CHAIN; LIMITS OF DETECTION; MICROBEAD; REACTION PERIOD; SANDWICH ASSAYS; SANDWICH IMMUNOASSAY; SEROTYPE;

ASSAYS; CELL MEMBRANES; FLOW CYTOMETRY; FLUORESCENCE; MULTIPLEXING;

ANTIBODIES;

BOTULINUM TOXIN A; BUFFER; FLUORESCENT DYE; TOXIN ANTIBODY;

ANTIBODY LABELING; ARTICLE; AUTOMATION; CENTRIFUGE; CONTROLLED STUDY; ENZYME IMMUNOASSAY; FLOW CYTOMETRY; FLUORESCENCE; HEAVY CHAIN; PERFUSION; SAMPLE; SOLUTION AND SOLUBILITY; TUBE;

ANIMALS; ANTIBODIES; AUTOMATIC DATA PROCESSING; AUTOMATION; BIOSENSING TECHNIQUES; BOTULINUM TOXIN TYPE A; BUFFERS; EPITOPES; FLOW CYTOMETRY; HUMANS; IMMOBILIZED PROTEINS; MICE; MICROSPHERES; MOLECULAR WEIGHT; PEPTIDE FRAGMENTS; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT PROTEINS; TIME FACTORS;

EID: 67650751073     PISSN: 00032700     EISSN: None     Source Type: Journal    
DOI: 10.1021/ac9006914     Document Type: Article

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