Dual inducible expression of recombinant GFP and targeted antisense RNA in Lactococcus lactis

Plasmid

Volumn 62, Issue 2, 2009, Pages 108-118

  Oddone, Gian M ^a   Mills, David A ^b   Block, David E ^a,b  

^a University of California   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Antisense; GFP; Lactococcus lactis; Metabolic engineering; Nisin; Recombinant protein expression

Indexed keywords

COMPLEMENTARY RNA; ENDOPEPTIDASE CLP; GREEN FLUORESCENT PROTEIN; NISIN; RECOMBINANT PROTEIN;

ARTICLE; BACTERIAL STRAIN; CONTROLLED STUDY; EXPRESSION VECTOR; GENE EXPRESSION SYSTEM; GENE SILENCING; HIGH THROUGHPUT SCREENING; LACTIC ACID BACTERIUM; LACTOCOCCUS LACTIS; METABOLIC ENGINEERING; NONHUMAN; PROTEIN ENGINEERING; PROTEIN EXPRESSION; SHUTTLE VECTOR; STRAIN IMPROVEMENT;

ENDOPEPTIDASE CLP; GREEN FLUORESCENT PROTEINS; LACTOCOCCUS LACTIS; RECOMBINANT PROTEINS; RNA, ANTISENSE; RNA, MESSENGER;

ESCHERICHIA COLI; LACTOCOCCUS LACTIS; SACCHAROMYCES CEREVISIAE;

EID: 67650734953     PISSN: 0147619X     EISSN: 10959890     Source Type: Journal    
DOI: 10.1016/j.plasmid.2009.06.002     Document Type: Article

References (28)

1. Konings W.N. The cell membrane and the struggle for life of lactic acid bacteria. Antonie Van Leeuwenhoek Int. J. Gen. Mol. Microbiol. 1-4 (2002) 3-27
2. Wells J.M., and Mercenier A. Mucosal delivery of therapeutic and prophylactic molecules using lactic acid bacteria. Nat. Rev. Microbiol. 5 (2008) 349-362
3. Norton P.M., Brown H.W., Wells J.M., Macpherson A.M., Wilson P.W., and Le Page R.W. Factors affecting the immunogenicity of tetanus toxin fragment C expressed in Lactococcus lactis. FEMS Immunol. Med. Microbiol. 2-3 (1996) 167-177
4. Wells J.M., and Mercenier A. Lactic acid bacteria as mucosal delivery vehicles. The Genetics of Lactic Acid Bacteria (2003), Kluwer Academic/Plenum Publishers, New York 260-291
5. Berlec A., Tompa G., Slapar N., Fonovic U.P., Rogelj I., and Strukelj B. Optimization of fermentation conditions for the expression of sweet-tasting protein brazzein in Lactococcus lactis. Lett. Appl. Microbiol. (2008) 227-231
6. Mierau I., Olieman K., Mond J., and Smid E.J. Optimization of the Lactococcus lactis nisin-controlled gene expression system NICE for industrial applications. Microb. Cell Fact. 1 (2005) 16-28
7. Oddone G.M., Lan C.Q., Rawsthorne H., Mills D.A., and Block D.E. Optimization of fed-batch production of the model recombinant protein GFP in Lactococcus lactis. Biotechnol. Bioeng. 6 (2007) 1127-1138
8. Kim J.H., and Mills D.A. Improvement of a nisin-inducible expression vector for use in lactic acid bacteria. Plasmid 3 (2007) 275-283
9. Oddone G.M., Mills D.A., and Block D.E. Incorporation of nisI-mediated nisin immunity improves vector-based nisin-controlled gene expression in lactic acid bacteria. Plasmid 3 (2009) 151-158
10. Mierau I., and Kleerebezem M. 10 years of the nisin-controlled gene expression system (NICE) in Lactococcus lactis. Appl. Microbiol. Biotechnol. (2005) 1-13
11. Cortes-Perez N.G., Poquet I., Oliveira M., Gratadoux J.J., Madsen S.M., Miyoshi A., Corthier G., Azevedo V., Langella P., and Bermudez-Humaran L.G. Construction and characterization of a Lactococcus lactis strain deficient in intracellular CIpP and extracellular HtrA proteases. Microbiol-SGM (2006) 2611-2618
12. Simons R.W., and Kleckner N. Biological regulation by antisense Rna in prokaryotes. Annu. Rev. Genet. (1988) 567-600
13. Rasmussen L.C.V., Sperling-Petersen H.U., and Mortensen K.K. Hitting bacteria at the heart of the central dogma: sequence-specific inhibition. Microb. Cell Fact. (2007)
14. Bouazzaoui K., and LaPointe G. Use of antisense RNA to modulate glycosyltransferase gene expression and exopolysaccharide molecular mass in Lactobacillus rhamnosus. J. Microbiol. Methods 2 (2006) 216-225
15. McGrath S., Fitzgerald G.F., and van Sinderen D. Improvement and optimization of two engineered phage resistance mechanisms in Lactococcus lactis. Appl. Environ. Microbiol. 2 (2001) 608-616
16. Sturino J.M., and Klaenhammer T.R. Antisense RNA targeting of primase interferes with bacteriophage replication in Streptococcus thermophilus. Appl. Environ. Microbiol. 3 (2004) 1735-1743
17. Johnson J.R., Lockman H.A., Owens K., Jelacic S., and Tarr P.I. High-frequency secondary mutations after suicide-driven allelic exchange mutagenesis in extraintestinal pathogenic Escherichia coli. J. Bacteriol. 17 (2003) 5301-5305
18. Kim J.Y.H., and Cha H.J. Down-regulation of acetate pathway through antisense strategy in Escherichia coli: Improved foreign protein production. Biotechnol. Bioeng. 7 (2003) 841-853
19. Kemmer C., and Neubauer P. Antisense RNA based down-regulation of RNaseE in E-coli. Microb. Cell Fact. (2006)
20. Ausubel F.M., Brent R., Kingston R.E., Moore D.D., Seidman J.G., Smith J.A., and Struhl K. Current Protocols in Molecular Biology (1995), Wiley, New York, NY
21. Chiera J.M., Lindbo J.A., and Finer J.J. Quantification and extension of transient GFP expression by the co-introduction of a suppressor of silencing. Transgenic Res. 6 (2008) 1143-1154
22. Niu Y., Kong J., and Xu Y. A novel GFP-fused eukaryotic membrane protein expression system in Lactococcus lactis and its application to overexpression of an elongase. Curr. Microbiol. 5 (2008) 423-428
23. Wang S.H., Xie W.J., Rylander M.N., Tucker P.W., Aggarwal S., and Diller K.R. HSP70 kinetics study by continuous observation of HSP-GFP fusion protein expression on a perfusion heating stage. Biotechnol. Bioeng. 1 (2008) 146-154
24. Sela D.A., Chapman J., Adeuya A., Kim J.H., Chen F., Whitehead T.R., Lapidus A., Rokhsar D.S., Lebrilla C.B., German J.B., Price N.P., Richardson P.M., and Mills D.A. The genome sequence of Bifidobacterium longum subsp. infantis reveals adaptations for milk utilization within the infant microbiome. Proc. Natl. Acad. Sci. USA 48 (2008) 18964-18969
25. Frees D., and Ingmer H. ClpP participates in the degradation of misfolded protein in Lactococcus lactis. Mol. Microbiol. 1 (1999) 79-87
26. Frees D., Savijoki K., Varmanen P., and Ingmer H. Clp ATPases and ClpP proteolytic complexes regulate vital biological processes in low GC, gram-positive bacteria. Mol. Microbiol. 5 (2007) 1285-1295
27. Xie Y., Chou L.s., Cutler A., and Weimer B. DNA macroarray profiling of Lactococcus lactis subsp. lactis IL1403 gene expression during environmental stresses. Appl. Environ. Microbiol. 11 (2004) 6738-6747
28. Ji Y.D., Zhang B., Van Horn S.F., Warren P., Woodnutt G., Burnham M.K.R., and Rosenberg M. Identification of critical staphylococcal genes using conditional phenotypes generated by antisense RNA. Science 5538 (2001) 2266-2269