Factors affecting the immunogenicity of tetanus toxin fragment C expressed in Lactococcus lactis

FEMS Immunology and Medical Microbiology

Volumn 14, Issue 2-3, 1996, Pages 167-177

  Norton, Pamela M ^a,b   Brown, Henry W G ^a   Wells, Jeremy M ^a   Macpherson, Angela M ^a   Wilson, Peter W ^a   Le Page, Richard W F ^a  

^a UNIVERSITY OF CAMBRIDGE   (United Kingdom)

^b INSTITUTE FOR ANIMAL HEALTH   (United Kingdom)

Author keywords

Bacterial vaccine vector; Lactococcus lactis; Targeting antigen; Tetanus toxin fragment C; Vaccine

Indexed keywords

TETANUS TOXIN; TETANUS TOXIN FRAGMENT C; UNCLASSIFIED DRUG;

ARTICLE; CONTROLLED STUDY; IMMUNOGENICITY; LACTOCOCCUS LACTIS; MOUSE; NONHUMAN; PRIORITY JOURNAL;

EID: 0029889745     PISSN: 09288244     EISSN: None     Source Type: Journal    
DOI: 10.1016/0928-8244(96)00028-4     Document Type: Article

References (27)

1. Aldovini, A. and Young, R.A. (1991) Humoral and cell-mediated immune responses to live recombinant BCG-HIV vaccines. Nature 351, 479-482.
2. Chatfield, S.N., Charles, I.G., Makoff, A.J., Oxer, M.D., Dougan, G., Pickard, D., Slater, D. and Fairweather, N.F. (1992) Use of the nirB promoter to direct the stable expression of heterologous antigens in Salmonella oral vaccine strains: development of a single-dose oral tetanus vaccine. Bio/Technology 10, 888-892.
3. Dougan, G., Roberts, M., Douce, G., Londono, P., Hormaeche, C., Harrison, J. and Chatfield, S. (1993) The genetics of Salmonella and vaccine development. In: Biology of Salmonella (Cabello Hormaeche, F., Mastroeni, P. and Bonina, L., Eds.), Plenum Press.
4. Stover, C.K., Bansal, G.-P., Hanson, M.S., Burlein, J.E., Palazynski, S.R., Young, J.F., Koenig, S., Young, D.B., Sadziena, A. and Barbour, A.G. (1993) Protective immunity elicited by recombinant bacille Calmette-Guerin (BCG) expressing outer surface protein A (OspA) lipoprotein: a candidate Lyme disease vaccine. J. Exp. Med. 178, 197-209.
5. Yasutomi-Y., Koenig, S., Haun, S.S., Stover, C.K., Jackson, R.K., Conard, P., Conley, A.S., Emini, E.A. and Furst, T.R. (1993) Immunisation with recombinant BCG-SIV elicits SIV-specific cytotoxic T lymphocytes in rhesus monkeys. J. Immunol. 150, 3101-3107.
6. Mestecky, J. and Eldridge, J.H. (1991) Targetting and controlled release of antigens for the effective induction of secretory antibody responses. Curr. Opin. Immunol. 3, 492-495.
7. Norton, P.M., Brown, H.W.G. and Le Page, R.W.F. (1994) The immune response to Lactococcus lactis: Implications for its use as a vaccine delivery vehicle. FEMS Microbiol. Lett. 120, 249-256.
8. Wells, J.M., Wilson, P.W., Norton, P.M., Gasson, M.J. and Le Page, R.W.F. (1993) Lactococcus lactis: high level expression of tetanus toxin fragment C and protection against lethal challenge. Mol. Microbiol 8, 1155-1162.
9. Wells, J.M., Wilson, P.W., Norton, P.M. and Le Page, R.W.F. (1993) A model system for the investigation of heterologous protein secretion pathways in Lactococcus lactis. Appl. Environ. Microbiol. 59, 3954-3959.
10. Helting, T.B. and Zwister, O. (1977) Structure of tetanus toxin: I breakdown of the toxin molecule and discrimination between polypeptide fragments. J. Biol. Chem. 252, 187-193.
11. Helting, T.B. and Nau, H.-H. (1984) Analysis of the immune response to papain digestion products of tetanus toxin. Acta Pathol. Microbiol. Scand. Sect. C92, 59-63.
12. Fairweather, N.F., et al. (1987) Immunisation of mice against tetanus toxin with fragments of tetanus toxin synthesised in E. coli. Infect. Immun. 55, 2541-2545.
13. Leclerc, C., Lo-man, R., Martineau, P., Clement, J.M. and Hofnung, M. (1994) Immunogenicity of viral B-and T-cell epitopes expressed in recombinant bacterial proteins. Int. Rev. Immunol. 11, 123-132.
14. Leclerc, C., Charbit, A., Martineau, P., Deriaud, E. and Hofnung, M. (1991) The cellular location of a foreign B cell epitope expressed by recombinant bacteria determines its T cell-independent or T cell-dependent characteristics. J. Immunol. 147(10), 3545-3552.
15. Charbit, A., Sobzak, E., Michel, M.-L., Molla, A., Tiollais, P. and Hofnung, M. (1987) Presentation of two epitopes of the preS2 region of Hepatitis B virus on live recombinant bacteria. J. Immunol. 139, 1658-1664.
16. Kiwaki, M., Ikemura, H., Shimizu-Kadota, M. and Hirashima, A. (1989) Molecular characterisation of a cell wall-associated proteinase gene from Streptococcus lactis NCDO763. Mol. Microbiol. 3, 359-369.
17. Casadaban, M.J. and Cohen, S.N. (1980) Analysis of gene control signals by DNA fusion and cloning in Escherichia coli. J. Mol. Biol. 138, 179-207.
18. Maeda, S. and Gasson, M.J. (1986) Cloning, expression and location of the Streptococcus lactis gene for phospho-β-D-galactosidase. J. Gen. Microbiol. 132, 331-340.
19. Eckert, A.K. and Kunkel, T.A. (1990) High fidelity DNA synthesis by the Thermus aquaticus DNA polymerase. Nucleic Acids Res. 18, 3739-3744.
20. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual. Cold Spring Harbour Laboratory Press, Cold Spring Harbour.
21. Wells, J.M., Wilson, P.W. and Le Page, R.W.F. (1993) Improved cloning vectors and transformation procedure for Lactococcus lactis J. Appl. Bact. 74, 629-636.
22. Laemmli, U.K. (1970) Cleavage of structural protein during the assembly of the head of bacteriophage T4. Nature 227, 680-685.
23. Burnette, W.H. (1981) Western blotting: electrophoretic transfer of proteins from SDS polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein. Anal. Biochem. 112, 195-203.
24. Halpern, J.L., Habig, W.H., Neale, E.A. and Stibitz, S. (1990) Cloning and expression of functional fragment C of tetanus toxin. Infect. Immun. 58, 1004-1009.
25. Forrest, B.D. (1992) Indirect measurement of intestinal immune responses to an orally administered attenuated bacterial vaccine. Infect. Immun. 60, 2023-2029.
26. Naylor, G.R.E. (1983) Incubation period and other features of food-borne and water-borne outbreaks of typhoid fever in relation to pathogenesis and genetics of resistance. Lancet i, 864-866.
27. Cardenas, L. and Clements, J.D. (1993) Stability, immunogenicity and expression of foreign antigens in bacterial vaccine vectors. Vaccine 11(2), 126-134.