A new strategy for constructing in vitro replication-competent 1.3 copies of hepatitis B virus genome

Journal of Virological Methods

Volumn 161, Issue 1, 2009, Pages 63-69

  Hu, Jie li ^a   Cui, Jing ^a   Deng, Xiao yan ^a   Zhang, Wen lu ^a   Li, Qing ling ^b   Guo, Jin jun ^b   Zeng, Ai zhong ^b   Huang, Ai long ^a  

^a BEIJING UNIVERSITY OF POSTS AND TELECOMMUNICATIONS   (China)

^b CHONGQING MEDICAL UNIVERSITY   (China)

Author keywords

HBV 1.3 genome; Hepatitis B virus; In vitro replication

Indexed keywords

VIRUS DNA;

ARTICLE; CASE REPORT; CELL STRAIN HEPG2; CONTROLLED STUDY; GENOTYPE; HEPATITIS B; HEPATITIS B VIRUS; HUMAN; HUMAN CELL; IN VITRO STUDY; NONHUMAN; PRIORITY JOURNAL; VIRUS GENOME; VIRUS ISOLATION; VIRUS REPLICATION; VIRUS STRAIN;

BLOTTING, SOUTHERN; CELL LINE, TUMOR; DNA, VIRAL; GENOME, VIRAL; HEPATITIS B VIRUS; HEPATOCYTES; HUMANS; VIRUS REPLICATION;

HEPATITIS B VIRUS;

EID: 67650677919     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jviromet.2009.05.017     Document Type: Article

References (34)

1. Chin R., Shaw T., Torresi J., Sozzi V., Trautwein C., Bock T., Manns M., Isom H., Furman P., and Locarnini S. In vitro susceptibilities of wild-type or drug-resistant hepatitis B virus to (-)-beta-d-2,6-diaminopurine dioxolane and 2′-fluoro-5-methyl-beta-l-arabinofuranosyluracil. Antimicrob. Agents Chemother. 45 (2001) 2495-2501
2. De Maddalena C., Giambelli C., Tanzi E., Colzani D., Schiavini M., Milazzo L., Bernini F., Ebranati E., Cargnel A., Bruno R., Galli M., and Zehender G. High level of genetic heterogeneity in S and P genes of genotype D hepatitis B virus. Virology 365 (2007) 113-124
3. Delaney W.T., Bartholomeusz A., and Locarnini S.A. Evolving therapies for the treatment of chronic hepatitis B virus infection. Expert Opin. Investig. Drugs 11 (2002) 169-187
4. Durantel D., Brunelle M.N., Gros E., Carrouee-Durantel S., Pichoud C., Villet S., Trepo C., and Zoulim F. Resistance of human hepatitis B virus to reverse transcriptase inhibitors: from genotypic to phenotypic testing. J. Clin. Virol. 34 Suppl 1 (2005) S34-43
5. Durantel D., Carrouee-Durantel S., Werle-Lapostolle B., Brunelle M.N., Pichoud C., Trepo C., and Zoulim F. A new strategy for studying in vitro the drug susceptibility of clinical isolates of human hepatitis B virus. Hepatology 40 (2004) 855-864
6. Ganem D., and Prince A.M. Hepatitis B virus infection-natural history and clinical consequences. N. Engl. J. Med. 350 (2004) 1118-1129
7. Guha C., Mohan S., Roy-Chowdhury N., and Roy-Chowdhury J. Cell culture and animal models of viral hepatitis. Part I. Hepatitis B. Lab Anim. (NY) 33 (2004) 37-46
8. Gunther S., Li B.C., Miska S., Kruger D.H., Meisel H., and Will H. A novel method for efficient amplification of whole hepatitis B virus genomes permits rapid functional analysis and reveals deletion mutants in immunosuppressed patients. J. Virol. 69 (1995) 5437-5444
9. Guo K.J., and Bowden D.S. Digoxigenin-labeled probes for the detection of hepatitis B virus DNA in serum. J. Clin. Microbiol. 29 (1991) 506-509
10. Keasler V.V., Hodgson A.J., Madden C.R., and Slagle B.L. Enhancement of hepatitis B virus replication by the regulatory X protein in vitro and in vivo. J. Virol. 81 (2007) 2656-2662
11. Lee W.M. Hepatitis B virus infection. N. Engl. J. Med. 337 (1997) 1733-1745
12. Marion P.L., and Robinson W.S. Hepadna viruses: hepatitis B and related viruses. Curr. Top. Microbiol. Immunol. 105 (1983) 99-121
13. Medley G.F., Lindop N.A., Edmunds W.J., and Nokes D.J. Hepatitis-B virus endemicity: heterogeneity, catastrophic dynamics and control. Nat. Med. 7 (2001) 619-624
14. Nassal M., Junker-Niepmann M., and Schaller H. Translational inactivation of RNA function: discrimination against a subset of genomic transcripts during HBV nucleocapsid assembly. Cell 63 (1990) 1357-1363
15. Newbold J.E., Xin H., Tencza M., Sherman G., Dean J., Bowden S., and Locarnini S. The covalently closed duplex form of the hepadnavirus genome exists in situ as a heterogeneous population of viral minichromosomes. J. Virol. 69 (1995) 3350-3357
16. Norder H., Courouce A.M., Coursaget P., Echevarria J.M., Lee S.D., Mushahwar I.K., Robertson B.H., Locarnini S., and Magnius L.O. Genetic diversity of hepatitis B virus strains derived worldwide: genotypes, subgenotypes, and HBsAg subtypes. Intervirology 47 (2004) 289-309
17. Parekh S., Zoulim F., Ahn S.H., Tsai A., Li J., Kawai S., Khan N., Trepo C., Wands J., and Tong S. Genome replication, virion secretion, and e antigen expression of naturally occurring hepatitis B virus core promoter mutants. J. Virol. 77 (2003) 6601-6612
18. Park S.G., Kim Y., Park E., Ryu H.M., and Jung G. Fidelity of hepatitis B virus polymerase. Eur. J. Biochem. 270 (2003) 2929-2936
19. Pollack J.R., and Ganem D. An RNA stem-loop structure directs hepatitis B virus genomic RNA encapsidation. J. Virol. 67 (1993) 3254-3263
20. Scaglioni P.P., Melegari M., and Wands J.R. Posttranscriptional regulation of hepatitis B virus replication by the precore protein. J. Virol. 71 (1997) 345-353
21. Seeger C., and Mason W.S. Hepatitis B virus biology. Microbiol. Mol. Biol. Rev. 64 (2000) 51-68
22. Shevchuk N.A., Bryksin A.V., Nusinovich Y.A., Cabello F.C., Sutherland M., and Ladisch S. Construction of long DNA molecules using long PCR-based fusion of several fragments simultaneously. Nucleic Acids Res. 32 (2004) e19
23. Summers J., and Mason W.S. Replication of the genome of a hepatitis B-like virus by reverse transcription of an RNA intermediate. Cell 29 (1982) 403-415
24. Tang H., Delgermaa L., Huang F., Oishi N., Liu L., He F., Zhao L., and Murakami S. The transcriptional transactivation function of HBx protein is important for its augmentation role in hepatitis B virus replication. J. Virol. 79 (2005) 5548-5556
25. Tenney D.J., Levine S.M., Rose R.E., Walsh A.W., Weinheimer S.P., Discotto L., Plym M., Pokornowski K., Yu C.F., Angus P., Ayres A., Bartholomeusz A., Sievert W., Thompson G., Warner N., Locarnini S., and Colonno R.J. Clinical emergence of entecavir-resistant hepatitis B virus requires additional substitutions in virus already resistant to Lamivudine. Antimicrob. Agents Chemother. 48 (2004) 3498-3507
26. Tenney D.J., Rose R.E., Baldick C.J., Levine S.M., Pokornowski K.A., Walsh A.W., Fang J., Yu C.F., Zhang S., Mazzucco C.E., Eggers B., Hsu M., Plym M.J., Poundstone P., Yang J., and Colonno R.J. Two-year assessment of entecavir resistance in Lamivudine-refractory hepatitis B virus patients reveals different clinical outcomes depending on the resistance substitutions present. Antimicrob. Agents Chemother. 51 (2007) 902-911
27. Villeneuve J.P., Durantel D., Durantel S., Westland C., Xiong S., Brosgart C.L., Gibbs C.S., Parvaz P., Werle B., Trepo C., and Zoulim F. Selection of a hepatitis B virus strain resistant to adefovir in a liver transplantation patient. J. Hepatol. 39 (2003) 1085-1089
28. Wang G.H., and Seeger C. Novel mechanism for reverse transcription in hepatitis B viruses. J. Virol. 67 (1993) 6507-6512
29. Wang G.H., Zoulim F., Leber E.H., Kitson J., and Seeger C. Role of RNA in enzymatic activity of the reverse transcriptase of hepatitis B viruses. J. Virol. 68 (1994) 8437-8442
30. Xu Z., Yen T.S., Wu L., Madden C.R., Tan W., Slagle B.L., and Ou J.H. Enhancement of hepatitis B virus replication by its X protein in transgenic mice. J. Virol. 76 (2002) 2579-2584
31. Yang H., Westland C., Xiong S., and Delaney W.E.T. In vitro antiviral susceptibility of full-length clinical hepatitis B virus isolates cloned with a novel expression vector. Antiviral Res. 61 (2004) 27-36
32. Yatsuji H., Noguchi C., Hiraga N., Mori N., Tsuge M., Imamura M., Takahashi S., Iwao E., Fujimoto Y., Ochi H., Abe H., Maekawa T., Tateno C., Yoshizato K., Suzuki F., Kumada H., and Chayama K. Emergence of a novel lamivudine-resistant hepatitis B virus variant with a substitution outside the YMDD motif. Antimicrob. Agents Chemother. 50 (2006) 3867-3874
33. Yolov A.A., and Shabarova Z.A. Constructing DNA by polymerase recombination. Nucleic Acids Res. 18 (1990) 3983-3986
34. Zoulim F. In vitro models for studying hepatitis B virus drug resistance. Semin. Liver Dis. 26 (2006) 171-180