Development and validation of a fluorogenic assay to measure separase enzyme activity

Analytical Biochemistry

Volumn 392, Issue 2, 2009, Pages 133-138

  Basu, Dipanjan ^a   Zhang, Nenggang ^a   Panigrahi, Anil K ^a   Horton, Terzah M ^a   Pati, Debananda ^a  

^a BAYLOR COLLEGE OF MEDICINE   (United States)

Author keywords

Enzyme assay; Leukemia; Rad21; Securin; Separase

Indexed keywords

CELL CULTURE; CHLOROACETIC ACID; DISEASES; MAMMALS;

ENZYME ASSAYS; LEUKEMIA; RAD21; SECURIN; SEPARASE;

ENZYME ACTIVITY;

AMINO ACID DERIVATIVE; COHESIN; COUMARIC ACID; PEPTIDE; PROTEIN RAD21; SEPARASE; UNCLASSIFIED DRUG;

ARTICLE; CELL CYCLE PROGRESSION; CONTROLLED STUDY; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME ASSAY; ENZYME SUBSTRATE; FLUOROGENIC ASSAY; HUMAN; HUMAN CELL; LEUKEMIA; MITOSIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN CLEAVAGE; TUMOR CELL; VALIDATION STUDY;

MUS;

EID: 67650484189     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2009.05.046     Document Type: Article

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