Delivery of phosphorodiamidate morpholino antisense oligomers in cancer cells

Methods in Molecular Biology

Volumn 542, Issue , 2009, Pages 351-361

  Devi, Gayathri R ^a  

^a DUKE CANCER INSTITUTE   (United States)

Author keywords

Apoptosis; delivery; fluorescence; immunoblots; morpholino; oligonucleotides; phosphorodiamidate; PMO

Indexed keywords

MORPHOLINE DERIVATIVE; PHOSPHORODIAMIDATE MORPHOLINO OLIGOMER; TUMOR PROTEIN;

ARTICLE; GENE TRANSFER; HUMAN; METABOLISM; NEOPLASM; TUMOR CELL LINE;

CELL LINE, TUMOR; GENE TRANSFER TECHNIQUES; HUMANS; MORPHOLINES; NEOPLASM PROTEINS; NEOPLASMS;

EID: 67650312331     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-59745-561-9_19     Document Type: Article

References (38)

1. Nasevicius A , Ekker SC.2000) Effective targeted gene "knockdown" in zebrafish Nat Genet 26 : 216-220
2. Sahu NK , Shilakari G , Nayak A , Kohli DV.2007) Antisense technology: a selective tool for gene expression regulation and gene targeting Curr Pharm Biotechnol 8 : 291-304
3. Tillman LG , Geary RS , Hardee GE.2008) Oral delivery of antisense oligonucleotides in man J Pharm Sci 97 : 225-236
4. Stein CA , Benimetskaya L , Mani S.2005) Antisense strategies for oncogene inactivation Semin Oncol 32 : 563-572
5. Ghosh C , Stein D , Weller D , Iversen P.2000) Evaluation of antisense mechanisms of action Methods Enzymol 313 : 135-143
6. Arora V , Devi GR , Iversen PL.2004) Neutrally charged phosphorodiamidate morpholino antisense oligomers: uptake, efficacy and pharmacokinetics Curr Pharm Biotechnol 5 : 431-439
7. Summerton J.1999) Morpholino antisense oligomers: the case for an RNase H-independent structural type Biochim Biophys Acta 1489 : 141-158
8. Summerton J , Weller D.1997) Morpholino antisense oligomers: design, preparation, and properties Antisense Nucleic Acid Drug Dev 7 : 187-195
9. Giles RV , Spiller DG , Clark RE , Tidd DM.1999) Antisense morpholino oligonucleotide analog induces missplicing of C-myc mRNA Antisense Nucleic Acid Drug Dev 9 : 213-220
10. Aartsma-Rus A , Van Ommen GJ.2007) Antisense- mediated exon skipping: a versatile tool with therapeutic and research applications RNA 13 : 1609-1624
11. Stein CA.1997) Controversies in the cellular pharmacology of oligodeoxynucleotides Antisense Nucleic Acid Drug Dev 7 : 207-209
12. Hans SS , Hans BA , Dhillon R , Dmuchowski C , Glover J.1998) Effect of dopamine on renal function after arteriography in patients with pre-existing renal insufficiency Am Surg 64 : 432-436
13. Biessen EA , Vietsch H , Kuiper J , Bijsterbosch MK , Berkel TJ.1998) Liver uptake of phosphodiester oligodeoxynucleotides is mediated by scavenger receptors Mol Pharmacol 53 : 262-269
14. Yakubov LA , Deeva EA , Zarytova VF , IVanova EM , Ryte AS , Yurchenko LV , et al.1989) Mechanism of oligonucleotide uptake by cells: involvement of specific receptors? Proc Natl Acad Sci USA 86 : 6454-6458
15. Loke SL , Stein CA , Zhang XH , Mori K , Nakanishi M , Subasinghe C , et al.1989) Characterization of oligonucleotide transport into living cells Proc Natl Acad Sci USA 86 : 3474-3478
16. Benimetskaya L , Loike JD , Khaled Z , Loike G , Silverstein SC , Cao L , et al.1997) Mac-1 (CD11b/CD18) is an oligodeoxynucleotidebinding protein Nat Med 4 : 414-420
17. Laktionov PP , Dazard JE , Vives E , Rykova EY , Piette J , Vlassov VV , et al.1999) Characterization of membrane oligonucleotide-binding proteins and oligonucleotide uptake in keratinocytes Nucleic Acids Res 27 : 2315-2324
18. De Diesbach P , N'Kuli F , Delmee M , Courtoy PJ.2003) Infection by Mycoplasma hyorhinis strongly enhances uptake of antisense oligonucleotides: a reassessment of receptor-mediated endocytosis in the HepG2 cell line Nucleic Acids Res 31 : 886-892
19. Gray GD , Basu S , Wickstrom E.1997) Transformed and immortalized cellular uptake of oligodeoxynucleoside phosphorothioates, 3′-alkylamino oligodeoxynucleotides, 2 '?-O-methyl oligoribonucleotides, oligodeoxynucleoside methylphosphonates, and peptide nucleic acids Biochem Pharmacol 53 : 1465-1476
20. Levis JT , Butler WO , Tseng BY , Ts'o PO.1995) Cellular uptake of oligodeoxyribonucleoside methylphosphonates Antisense Res Dev 5 : 251-259
21. Delong RK , Miller PS.1996) Inhibition of human collagenase activity by antisense oligonucleoside methylphosphonates Antisense Nucleic Acid Drug Dev 6 : 273-280
22. Partridge M , Vincent A , Matthews P , Puma J , Stein D , Summerton J.1996) A simple method for delivering morpholino antisense oligos into the cytoplasm of cells Antisense Nucleic Acid Drug Dev 6 : 169-175
23. Ghosh C , Iversen PL.2000) Intracellular delivery strategies for antisense phosphorodiamidate morpholino oligomers Antisense Nucleic Acid Drug Dev 10 : 263-274
24. Morcos PA.2001) Achieving efficient delivery of morpholino oligos in cultured cells Genesis 30 : 94-102
25. Hudziak RM , Summerton J , Weller DD , Iversen PL.2000) Antiproliferative effects of steric blocking phosphorodiamidate morpholino antisense agents directed against c-myc Antisense Nucleic Acid Drug Dev 10 : 163-176
26. Arora V , Iversen PL.2000) Antisense oligonucleotides targeted to the p53 gene modulate liver regeneration in vivo Drug Metab Dispos 28 : 131-138
27. Arora V , Iversen PL.2001) Redirection of drug metabolism using antisense technology Curr Opin Mol Ther 3 : 249-257
28. Arora V , Knapp DC , Reddy MT , Weller DD , Iversen PL.2002) Bioavailability and efficacy of antisense morpholino oligomers targeted to c-myc and cytochrome P-450 3A2 following oral administration in rats J Pharm Sci 91 : 1009-1018
29. Arora V , Cate ML , Ghosh C , Iversen PL.2002) Phosphorodiamidate morpholino antisense oligomers inhibit expression of human cytochrome P450 3A4 and alter selected drug metabolism Drug Metab Dispos 7 : 757-762
30. Arora V , Hannah TL , Iversen PL , Brand RM.2002) Transdermal use of phosphorodiamidate morpholino oligomer AVI-4472 inhibits cytochrome P450 3A2 activity in male rats Pharm Res 10 : 465-1470
31. Devi GR , Oldenkamp JR , London CA , Iversen PL.2002) Inhibition of human chorionic gonadotropin beta-subunit modulates the mitogenic effect of c-myc in human prostate cancer cells Prostate 53 : 200-210
32. London CA , Sekhon HS , Arora V , Stein DA , Iversen PL , Devi GR.2003) A novel antisense inhibitor of MMP-9 attenuates angiogenesis, human prostate cancer cell invasion and tumorigenicity Cancer Gene Ther 10 : 823-832
33. Iversen PL , Arora V , Acker AJ , Mason DH , Devi GR.2003) Efficacy of antisense morpholino oligomer targeted to c-myc in prostate cancer xenograft murine model and a Phase I safety study in humans Clin Cancer Res 9 : 2510-2519
34. Amantana A , London CA , Iversen PL , Devi GR.2004) X-linked inhibitor of apoptosis protein inhibition induces apoptosis and enhances chemotherapy sensitivity in human prostate cancer cells Mol Cancer Ther 3 : 699-707
35. Sekhon HS, London CA, Sekhon M, Iversen PL, Devi GR.2007) c-MYC antisense phosphosphorodiamidate morpholino oligomerinhibits lung metastasis in a murine tumor model. Lung Cancer Dec 18 (Epub ahead of publication).
36. Suwanmanee T , Sierakowska H , Lacerra G , Svasti S , Kirby S , Walsh CE , et al.2002) Restoration of human beta-globin gene expression in murine and human IVS2-654 thalassemic erythroid cells by free uptake of antisense oligonucleotides Mol Pharmacol 62 : 545-553
37. Suwanmanee T , Sierakowska H , Fucharoen S , Kole R.2002) Repair of a splicing defect in erythroid cells from patients with beta-thalassemia/HbE disorder Mol Ther 6 : 718-726
38. Kang SH , Cho MJ , Kole R.1998 ) Upregulation of luciferase gene expression with antisense oligonucleotides: implications and applications in functional assay development Biochemistry 37 : 6235-6239