Ligand recognition by A-class Eph receptors: crystal structures of the EphA2 ligand-binding domain and the EphA2/ephrin-A1 complex

EMBO Reports

Volumn 10, Issue 7, 2009, Pages 722-728

  Himanen, Juha P ^a   Goldgur, Yehuda ^a   Miao, Hui ^b,c   Myshkin, Eugene ^b,c   Guo, Hong ^b,c   Buck, Matthias ^d   Nguyen, My ^a   Rajashankar, Kanagalaghatta R ^e   Wang, Bingcheng ^b,c   Nikolov, Dimitar B ^a  

^a MEMORIAL SLOAN KETTERING CANCER CENTER   (United States)

^b Rammelkamp Center for Education and Research   (United States)

^c CASE WESTERN RESERVE UNIVERSITY   (United States)

^d CASE WESTERN RESERVE UNIVERSITY SCHOOL OF MEDICINE   (United States)

^e ARGONNE NATIONAL LABORATORY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BENZOIC ACID DERIVATIVE; EPHRIN RECEPTOR A1; EPHRIN RECEPTOR A2;

ARTICLE; BINDING AFFINITY; CELL ADHESION; CELL INTERACTION; CONTROLLED STUDY; CRYSTAL STRUCTURE; DRUG DESIGN; DRUG PROTEIN BINDING; ENZYME ACTIVATION; ENZYME ASSAY; ENZYME SPECIFICITY; HUMAN; HUMAN CELL; LIGAND BINDING; MOLECULAR RECOGNITION; MUTAGENESIS; OLIGOMERIZATION; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN FUNCTION; PROTEIN PROTEIN INTERACTION; PROTEIN STRUCTURE;

AMINO ACID SEQUENCE; CELL LINE; CRYSTALLOGRAPHY, X-RAY; DRUG DESIGN; EPHRIN-A1; EPHRIN-A2; HUMANS; LIGANDS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; PROTEIN MULTIMERIZATION; PROTEIN STRUCTURE, SECONDARY; PROTEIN STRUCTURE, TERTIARY; RECEPTORS, EPH FAMILY; SEQUENCE ALIGNMENT; STATIC ELECTRICITY;

EID: 67650079305     PISSN: 1469221X     EISSN: 14693178     Source Type: Journal    
DOI: 10.1038/embor.2009.91     Document Type: Article

References (18)

1. Brunger AT et al (1998) Crystallography & NMR system: A new software suite for macromolecular structure determination. Acta Crystallogr D Biol Crystallogr 54: 905-921
2. Chrencik JE et al (2006a) Structure and thermodynamic characterization of the EphB4/Ephrin-B2 antagonist peptide complex reveals the determinants for receptor specificity. Structure 14 321-330
3. Chrencik JE, Brooun A, Kraus ML, Recht MI, Kolatkar AR, Han GW, Seifert JM, Widmer H, Auer M, Kuhn P (2006b) Structural and biophysical characterization of the EphB4*ephrinB2 protein-protein interaction and receptor specificity. J Biol Chem 281: 28185-28192
4. Davis S, Gale NW, Aldrich TH, Maisonpierre PC, Lhotak V, Pawson T, Goldfarb M, Yancopoulos GD (1994) Ligands for EPH-related receptor tyrosine kinases that require membrane attachment or clustering for activity. Science 266: 816-819
5. Goldgur Y, Paavilainen S, Nikolov D, Himanen JP (2009) Structure of the ligand-binding domain of the EphB2 receptor at 2 Å resolution. Acta Crystallogr Sect F Struct Biol Cryst Commun 63: 969-974
6. Guo H, Miao H, Gerber L, Singh J, Denning MF, Gilliam AC, Wang B (2006) Disruption of EphA2 receptor tyrosine kinase leads to increased susceptibility to carcinogenesis in mouse skin. Cancer Res 66 7050-7058
7. Himanen JP, Nikolov DB (2003) Eph signaling: A structural view. Trends Neurosci 26: 46-51
8. Himanen JP, Rajashankar KR, Lackmann M, Cowan CA, Henkemeyer M, Nikolov DB (2001) Crystal structure of an Eph receptor-ephrin complex. Nature 414: 933-938
9. Himanen JP et al (2004) Repelling class discrimination: Ephrin-A5 binds to and activates EphB2 receptor signaling. Nat Neurosci 7 501-509
10. Koolpe M, Dail M, Pasquale EB (2002) An ephrin mimetic peptide that selectively targets the EphA2 receptor. J Biol Chem 277: 46974-46979
11. Lackmann M, Oates AC, Dottori M, Smith FM, Do C, Power M, Kravets L, Boyd AW (1998) Distinct subdomains of the EphA3 receptor mediate ligand binding and receptor dimerization. J Biol Chem 273: 20228-20237
12. Miao H, Nickel CH, Cantley LG, Bruggeman LA, Bennardo LN, Wang B (2003) EphA kinase activation regulates HGF-induced epithelial branching morphogenesis. J Cell Biol 162: 1281-1292
13. Noberini R, Koolpe M, Peddibhotla S, Dahl R, Su Y, Cosford ND, Roth GP, Pasquale EB (2008) Small molecules can selectively inhibit ephrin binding to the EphA4 and EphA2 receptors. J Biol Chem 283: 29461-29472
14. Pabbisetty KB, Yue X, Li C, Himanen JP, Zhou R, Nikolov DB, Hu L (2007) Kinetic analysis of the binding of monomeric and dimeric ephrins to Eph receptors: Correlation to function in a growth cone collapse assay. Protein Sci 16: 355-361
15. Pasquale EB (2005) Eph receptor signalling casts a wide net on cell behaviour. Nat Rev Mol Cell Biol 6: 462-475
16. Project CCP4 (1994) The CCP4 suite: Programs for X-ray crystallography. Acta Crystallogr D 50: 760-763
17. Qin H, Shi J, Noberini R, Pasquale EB, Song J (2008) Crystal structure and NMR binding reveal that two small molecule antagonists target the high-affinity ephrin-binding channel of the EphA4 receptor. J Biol Chem 283: 29473-29484
18. Xu K, Rajashankar KR, Chan YP, Himanen JP, Broder CC, Nikolov DB (2008) Host cell recognition by the henipaviruses: Crystal structures of the Nipah G attachment glycoprotein and its complex with ephrin-B3. Proc Natl Acad Sci USA 105: 9953-9958