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Volumn 131, Issue 26, 2009, Pages 9189-9191

Reactivity-dependent PCR: Direct, solution-phase in vitro selection for bond formation

Author keywords

[No Author keywords available]

Indexed keywords

BOND CLEAVAGES; BOND FORMATION; BOND FORMING; BOND-CLEAVING; IN-VITRO; KEY COMPONENT; MULTIPLE SOLUTIONS; POTENTIAL SUBSTRATE; PROTEASE ACTIVITIES; SMALL MOLECULES; SOLID-PHASE;

EID: 67650001817     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja903084a     Document Type: Article
Times cited : (26)

References (42)
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    • Seminal reports: (a) Tuerk, C.; Gold, L. Science 1990, 249, 505.
  • 5
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  • 7
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    • In vitro selections of DNA-linked small molecules: (a) Wrenn, S. J.; Weisinger, R. M.; Halpin, D. R.; Harbury, P. B. J. Am. Chem. Soc. 2007, 129, 13137.
    • In vitro selections of DNA-linked small molecules: (a) Wrenn, S. J.; Weisinger, R. M.; Halpin, D. R.; Harbury, P. B. J. Am. Chem. Soc. 2007, 129, 13137.
  • 9
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    • Mock selections: (c) Doyon, J. B.; Snyder, T. M.; Liu, D. R. J. Am. Chem. Soc. 2003, 125, 12372.
    • Mock selections: (c) Doyon, J. B.; Snyder, T. M.; Liu, D. R. J. Am. Chem. Soc. 2003, 125, 12372.
  • 17
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    • Other tags may be used
    • (b) Seelig, B.; Jaschke, A. Chem. Biol. 1999, 6, 167. Other tags may be used.
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    • Seelig, B.1    Jaschke, A.2
  • 26
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    • Note that in contrast with a conventional selection in which unfit library members are discarded and sequences encoding surviving members are replicated in a subsequent step, RDPCR achieves the selective replication of only those sequences encoding desired library members
    • Note that in contrast with a conventional selection in which unfit library members are discarded and sequences encoding surviving members are replicated in a subsequent step, RDPCR achieves the selective replication of only those sequences encoding desired library members.
  • 28
    • 67650001355 scopus 로고    scopus 로고
    • Computation carried out using the Oligonucleotide Modeling Platform OMP, DNA Software, Inc
    • Computation carried out using the Oligonucleotide Modeling Platform (OMP, DNA Software, Inc.).
  • 29
    • 0027488624 scopus 로고    scopus 로고
    • For examples of phosphodiester bond formation-dependent PCR, see: (a) Bartel, D. P, Szostak, J. W. Science 1993, 261, 1411
    • For examples of phosphodiester bond formation-dependent PCR, see: (a) Bartel, D. P.; Szostak, J. W. Science 1993, 261, 1411.
  • 34
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    • Heck, R. F. Org. React. 1982, 27, 345. See also ref 5a.
    • Heck, R. F. Org. React. 1982, 27, 345. See also ref 5a.
  • 35
    • 67649905054 scopus 로고    scopus 로고
    • As little as 10 fmol of 9d and 9b could be carried through the process with similar results, suggesting that selections can be performed on a very small scale.
    • As little as 10 fmol of 9d and 9b could be carried through the process with similar results, suggesting that selections can be performed on a very small scale.
  • 36
    • 52649141086 scopus 로고    scopus 로고
    • For approaches to protease substrate profiling by derivitization of free α-amines, see: a
    • For approaches to protease substrate profiling by derivitization of free α-amines, see: (a) Mahrus, S.; Trinidad, J. C.; Barkan, D. T.; Sali, A.; Burlingame, A. L.; Wells, J. A. Cell 2008, 134, 866.
    • (2008) Cell , vol.134 , pp. 866
    • Mahrus, S.1    Trinidad, J.C.2    Barkan, D.T.3    Sali, A.4    Burlingame, A.L.5    Wells, J.A.6
  • 38
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  • 40
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    • DNA-templated amide bond formation with DMT-MM: (a) Li, X. Y.; Gartner, Z. J.; Tse, B. N.; Liu, D. R. J. Am. Chem. Soc. 2004, 126, 5090.
    • DNA-templated amide bond formation with DMT-MM: (a) Li, X. Y.; Gartner, Z. J.; Tse, B. N.; Liu, D. R. J. Am. Chem. Soc. 2004, 126, 5090.
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  • 42
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    • For a review of DTS, see: c
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    • Li, X.Y.1    Liu, D.R.2


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.