A new screening method based on yeast-expressed human dipeptidyl peptidase IV and discovery of novel inhibitors

Biotechnology Letters

Volumn 31, Issue 7, 2009, Pages 979-984

  Hu, Chen Xi ^a   Huang, Hui ^a   Zhang, Li ^a   Huang, You ^b   Shen, Zhu Fang ^a   Cheng, Ke Di ^a   Du, Guan Hua ^a   Zhu, Ping ^a  

^a PEKING UNION MEDICAL COLLEGE   (China)

^b NANKAI UNIVERSITY   (China)

Author keywords

Diatetes; DPP IV inhibitor; High throughput screening (HTS); Recombinant human dipeptidyl peptidase IV (rhDPP IV)

Indexed keywords

CACO-2 CELLS; DIATETES; DIPEPTIDYL PEPTIDASE; DPP-IV INHIBITOR; HIGH THROUGHPUT SCREENING; HIGH THROUGHPUT SCREENING (HTS); NEW STRATEGY; NEW SYSTEM; PICHIA PASTORIS; RECOMBINANT ENZYMES; RECOMBINANT HUMAN DIPEPTIDYL PEPTIDASE IV (RHDPP-IV); S/N RATIO; SCREENING METHODS; TYPE-2 DIABETES;

ELECTRIC BATTERIES; HIGH TEMPERATURE SUPERCONDUCTORS;

THROUGHPUT;

DIPEPTIDYL PEPTIDASE IV; DPP4 PROTEIN, HUMAN; ENZYME INHIBITOR; RECOMBINANT PROTEIN;

ARTICLE; BIOSYNTHESIS; DRUG ANTAGONISM; DRUG SCREENING; ENZYMOLOGY; GENETICS; HUMAN; IC 50; ISOLATION AND PURIFICATION; METHODOLOGY; PICHIA;

ANTIGENS, CD26; DRUG EVALUATION, PRECLINICAL; ENZYME INHIBITORS; HUMANS; INHIBITORY CONCENTRATION 50; PICHIA; RECOMBINANT PROTEINS;

PICHIA PASTORIS;

EID: 67649222542     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-009-9963-y     Document Type: Article

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