The influence of chitosan content in cationic chitosan/PLGA nanoparticles on the delivery efficiency of antisense 2′-O-methyl-RNA directed against telomerase in lung cancer cells

European Journal of Pharmaceutics and Biopharmaceutics

Volumn 72, Issue 2, 2009, Pages 358-369

  Taetz, S ^a   Nafee, N ^a   Beisner, J ^b   Piotrowska, K ^b   Baldes, C ^a   Murdter T E ^b   Huwer, H ^c   Schneider, M ^a   Schaefer, U F ^a   Klotz, U ^b   Lehr, C M ^a  

^a SAARLAND UNIVERSITY   (Germany)

^b UNIVERSITY OF TÜBINGEN   (Germany)

^c Shg Kliniken Volklingen   (Germany)

Author keywords

A549; Antisense 2 O methyl RNA; Calu 3; Chitosan PLGA nanoparticles; Lung cancer; Primary human alveolar epithelial cells; Telomerase

Indexed keywords

ANTISENSE 2' O METHYL RNA; CATION; CHITOSAN; CHITOSAN POLYGLACTIN NANOPARTICLE; COMPLEMENTARY RNA; NANOPARTICLE; POLYGLACTIN; SOLVENT; TELOMERASE; UNCLASSIFIED DRUG; WATER;

ANTISENSE THERAPY; ARTICLE; BINDING AFFINITY; CANCER CELL; CELL CULTURE; CELL LINE; COMPLEX FORMATION; CONFOCAL LASER MICROSCOPY; CONTROLLED STUDY; CULTURE MEDIUM; DRUG BINDING; DRUG SYNTHESIS; EMULSION; FLOW CYTOMETRY; GEL PERMEATION CHROMATOGRAPHY; HUMAN; HUMAN CELL; LUNG CANCER; MEASUREMENT; METHODOLOGY; PHOTON CORRELATION SPECTROSCOPY; PURIFICATION; ZETA POTENTIAL;

BASE SEQUENCE; CATIONS; CELL LINE, TUMOR; CHITOSAN; CHROMATOGRAPHY, GEL; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; DNA PRIMERS; HUMANS; LACTIC ACID; LUNG NEOPLASMS; MICROSCOPY, CONFOCAL; NANOPARTICLES; POLYGLYCOLIC ACID; POLYMERASE CHAIN REACTION; RNA, ANTISENSE; TELOMERASE;

EID: 67349137616     PISSN: 09396411     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ejpb.2008.07.011     Document Type: Article

References (24)

1. Blackburn E.H. Telomeres and telomerase: their mechanisms of action and the effects of altering their functions. FEBS Lett. 579 (2005) 859-862
2. Shay J.W., and Wright W.E. Senescence and immortalization: role of telomeres and telomerase. Carcinogenesis 26 (2004) 867-874
3. Piotrowska K., Kleideiter E., Mürdter T.E., Taetz S., Baldes C., Schaefer U.F., Lehr C.M., and Klotz U. Optimization of the TRAP assay to evaluate specificity of telomerase inhibitors. Lab. Invest. 85 (2005) 1565-1569
4. Pitts A.E., and Corey D.R. Inhibition of human telomerase by 2′-O-methyl-RNA. Proc. Natl. Acad. Sci. USA 95 (1998) 11549-11554
5. Herbert B., Pitts A.E., Baker S.I., Hamilton S.E., Wright W.E., Shay J.W., and Corey D.R. Inhibition of human telomerase in immortal human cells leads to progressive telomere shortening and cell death. Proc. Natl. Acad. Sci. USA 96 (1999) 14276-14281
6. Ravi Kumar M.N., Bakowsky U., and Lehr C.M. Preparation and characterization of cationic PLGA nanospheres as DNA carriers. Biomaterials 25 (2004) 1771-1777
7. Ravi Kumar M.N., Mohapatra S.S., Kong X., Jena P.K., Bakowsky U., and Lehr C.M. Cationic poly(lactide-co-glycolide) nanoparticles as efficient in vivo gene transfection agents. J. Nanosci. Nanotechnol. 4 (2005) 990-994
8. Nafee N., Taetz S., Schneider M., Schaefer U.F., and Lehr C.M. Chitosan-coated PLGA nanoparticles for DNA/RNA delivery: effect of the formulation parameters on complexation and transfection of antisense oligonucleotides. Nanomedicine 3 (2007) 173-183
9. Elbert K.J., Schafer U.F., Schafers H.J., Kim K.J., Lee V.H., and Lehr C.M. Monolayers of human alveolar epithelial cells in primary culture for pulmonary absorption and transport studies. Pharm. Res. 16 (1999) 601-608
10. Ehrhardt C., Kim K.J., and Lehr C.M. Isolation and culture of human alveolar epithelial cells. Methods Mol. Med. 107 (2005) 207-216
11. Mathias N.R., Timoszyk J., Stetsko P., Megill J., Smith R., and Wall D. Permeability characteristics of Calu-3 human bronchial epithelial cells: in vitro-in vitro correlation to predict lung absorption in rats. J. Drug Target. 10 (2002) 31-40
12. J. Beisner, M. Dong, S. Taetz, K. Piotrowska, E. Kledeideiter, G. Friedel, U.F. Schaefer, C.-M. Lehr, U. Klotz, T.E. Mürdter, Efficient telomerase inhibition in human non-small cell lung cancer cells by liposomal delivery of 2-O-methyl-RNA, J. Pharm. Sci. (2008), in press.
13. Sorlier P., Denuzière A., Viton C., and Domard A. Relation between the degree of acetylation and the electrostatic properties of chitin and chitosan. Biomacromolecules 2 (2001) 765-772
14. Lorenz M.R., Holzapfel V., Musyanovych A., Nothelfer K., Walther P., Frank H., Landfester K., Schrezenmeier H., and Mailänder V. Uptake of functionalized, fluorescent-labeled polymeric particles in different cell lines and stem cells. Biomaterials 27 (2006) 2820-2828
15. Gessner A., Lieske A., Paulke B., and Müller R. Influence of surface charge density on protein adsorption on polymeric nanoparticles: analysis by two-dimensional electrophoresis. Eur. J. Pharm. Biopharm. 54 (2002) 165-170
16. Lück M., Paulke B.R., Schröder W., Blunk T., and Müller R.H. Analysis of plasma protein adsorption on polymeric nanoparticles with different surface characteristics. J. Biomed. Mater. Res. 39 (1998) 478-485
17. Fang N., and Chan V. Interaction of liposome with immobilized chitosan during main phase transition. Biomacromolecules 4 (2003) 581-588
18. Löhbach C., Neumann D., Lehr C.M., and Lamprecht A. Human vascular endothelial cells in primary cell culture for the evaluation of nanoparticle bioadhesion. J. Nanosci. Nanotechnol. 6 (2006) 1-7
19. Huang M., Ma Z., Khor E., and Lim L.Y. Uptake of FITC-chitosan nanoparticles by A549 cells. Pharm. Res. 19 (2002) 1488-1494
20. Huang M., Khor E., and Lim L.Y. Uptake and cytotoxicity of chitosan molecules and nanoparticles: effects of molecular weight and degree of deacetylation. Pharm. Res. 21 (2004) 344-353
21. Fuchs S., Hollins A.J., Laue M., Schaefer U.F., Roemer K., Gumbleton M., and Lehr C.M. Differentiation of human alveolar epithelial cells in primary culture: morphological characterization and synthesis of caveolin-1 and surfactant protein-C. Cell Tissue Res. 311 (2003) 31-45
22. Gumbleton M. Caveolae as potential macromolecule trafficking compartments within alveolar epithelium. Adv. Drug Deliv. Rev. 49 (2001) 281-300
23. Bradbury N.A., Clark J.A., Watkins S.C., Widnell C.C., Smith H.S., and Bridges R.J. Characterization of the internalization pathways for the cystic fibrosis transmembrane conductance regulator. Am. J. Physiol. 276 (1999) L659-L668
24. Grenha A., Grainger C.I., Dailey L.A., Seijo B., Martin G.P., Remuñán-López C., and Forbes B. Chitosan nanoparticles are compatible with respiratory epithelial cells in vitro. Eur. J. Pharm. Sci. (2007)