High-yield expression and purification of a monotopic membrane glycosyltransferase

Protein Expression and Purification

Volumn 66, Issue 2, 2009, Pages 143-148

  Eriksson, Hanna M ^a,b   Persson, Karina ^c   Zhang, Shuguang ^b   Wieslander, Åke ^a  

^a STOCKHOLM UNIVERSITY   (Sweden)

^b Massachusetts Institute of Technology Cambridge   (United States)

^c UMEÅ UNIVERSITY   (Sweden)

Author keywords

Escherichia coli; Glycosyltransferase; Membrane protein; Monotopic; Optimization; Overexpression; Size exclusion chromatography

Indexed keywords

BACTERIAL PROTEIN; GLYCOSYLTRANSFERASE; MEMBRANE PROTEIN; RECOMBINANT PROTEIN;

ACHOLEPLASMA LAIDLAWII; ARTICLE; CELL MEMBRANE; CHEMISTRY; ENZYMOLOGY; ESCHERICHIA COLI; GEL CHROMATOGRAPHY; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; SOLUBILITY;

ACHOLEPLASMA LAIDLAWII; BACTERIAL PROTEINS; CELL MEMBRANE; CHROMATOGRAPHY, GEL; ESCHERICHIA COLI; GLYCOSYLTRANSFERASES; MEMBRANE PROTEINS; RECOMBINANT PROTEINS; SOLUBILITY;

ESCHERICHIA COLI;

EID: 67349125398     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2009.03.008     Document Type: Article

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