Can MTT be used to quantify the antioxidant activity of plant extracts?

Phytomedicine

Volumn 16, Issue 6-7, 2009, Pages 665-668

  Muraina, I A ^a   Suleiman, M M ^b   Eloff, J N ^b  

^a NATIONAL VETERINARY RESEARCH INSTITUTE   (Nigeria)

^b UNIVERSITY OF PRETORIA   (South Africa)

Author keywords

Antioxidant; DPPH; MTT; Plant extracts

Indexed keywords

1,1 DIPHENYL 2 PICRYLHYDRAZYL; BROMINE DERIVATIVE; PLANT EXTRACT;

ACCURACY; ANTIOXIDANT ACTIVITY; ARTICLE; BROTH DILUTION; CONCENTRATION RESPONSE; CONTROLLED STUDY; MEDICINAL PLANT; NIGERIA; NONHUMAN; PRIORITY JOURNAL; TERMINALIA LAXIFLORA; THIN LAYER CHROMATOGRAPHY;

ANTIOXIDANTS; PLANT EXTRACTS; TETRAZOLIUM SALTS; THIAZOLES;

EID: 67349114165     PISSN: 09447113     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.phymed.2008.11.005     Document Type: Article

References (23)

1. Aderogba M.A., McGaw L.J., Ogundaini A.O., and Eloff J.N. Antioxidant activity and cytotoxicity study of the flavonol glycosides from Bauhinia galpinii. Nat. Prod. Res. 21 (2007) 591-599
2. Ames B.N. Dietary carcinogens and anticarcinogens: oxygen radicals and degenerative diseases. Science 221 (1983) 1256-1264
3. Apak R., Guclu K., Ozyurek M., and Karademir S.E. A novel total antioxidant capacity index for dietary polyphenols, vitamins C and E, using their cupric ion reducing capability in the presence of neocuproine: CUPRAC method. J. Agric. Food Chem. 52 (2004) 7970-7981
4. Apak R., Guclu K., Ozyurek M., Karademir S.E., and Altun M. Total antioxidant capacity assay of human serum using copper(II)-neocuproine as chromogenic oxidant: the CUPRAC method. Free Radical Res. 39 (2005) 949
5. Arno M.B., Canao A., and Acosta M. The hydrophilic and lipophilic contribution to total antioxidant activity. Food Chem. 73 (2001) 239-244
6. Block G. The data support a role for antioxidants in reducing cancer risk. Nutr. Rev. 50 (1992) 207-213
7. 2+ ions: implications for their antioxidant properties. Biochem. J. 330 (1998) 1173-1178
8. Eloff J.N. A sensitive and quick microplate method to determine the minimal inhibitory concentration of plant extracts for bacteria. Planta Med. 64 (1998) 711-714
9. Foley S., Navaratnam S., McGarvey D.J., Land E.J., Truscott T.G., and Rice-Evans C.A. Singlet oxygen quenching and the redox properties of hydroxycinnamic acids. Free Radical Biol. Med. 26 (1999) 1202-1208
10. Halver J.E. Vitamin requirement study technique. J. Appl. Ichthyyol. 11 (1995) 215-244
11. Kotze M., and Eloff J.N. Extraction of antibacterial compounds from Combretum microphyllum (Combretaceae). S. Afr. J. Bot. 68 (2002) 62-67
12. Lodovici M., Guglielmi F., Casalini C., Meoni M., Cheynier V., and Dolara P. Antioxidant and radical scavenging properties in vitro of polyphenolic extracts from red wine. Eur. J. Nutr. 40 (2001) 74-77
13. Mazza G. Functional Foods and Nutraceuticals in Cancer Prevention (1998), Technomic publishing, Lancaster, PA
14. McDowell L.R., Williams S.N., Hidiroglou N., Njeru C.A., Hill G.M., Ochoa L., and Wilkinson N.S. Vitamin E supplementation for the ruminant. Anim. Feed Sci. Technol. 60 3-4 (1996) 273-296
15. Naik G.H., Priyardarsini K.I., Satav J.G., Banavalikar M.M., Sohoni D.P., Biyani M.K., and Mohan H. Comparative antioxidant activity of individual herbal components used in ayurvedic medicine. Phytochemistry 63 1 (2003) 97-104
16. Nockel C.F. Antioxidants improve cattle immunity following stress. Anim. Feed Sci. Technol. 62 (1996) 59-68
17. Ozyurt D., Demirata B., and Apak R. Determination of total antioxidant capacity by a new spectrophotometric method based on Ce (IV) reducing capacity measurement. Talata 71 (2007) 1155-1165
18. Re R., Pellegrini N., Proteggente A., Pannala A., Yang M., and Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolourization assay. Free Radical Biol. Med. 26 (1999) 1231-1237
19. Sanchez-Moreno C., Larrauri J.A., and Saura-Calixto F. A procedure to measure the antiradical efficiency of polyphenols. J. Sci. Food Agric. 76 (1998) 270-276
20. Surai P.F. Natural Antioxidants in Avian Nutrition and Reproduction (2002), Nottingham University Press pp. 5-9
21. Tseng T.H., Kao E.S., Chu C.Y., Chou F.P., Lin Wu H.W., and Wang C.J. Protective effects of dried flower extracts of Hibscus sabdariffa L. against oxidative stress in rat primary hepatocytes. Food Chem. Toxicol. 35 12 (1997) 1159-1164
22. Usoh I.F., Akpan E.J., Etim E.O., and Farombi E.O. Antioxidant action of dried flower extracts of Hibscus sabdariffa L. on sodium arsenite-induced oxidative stress rats. Pak. J. Nutr. 4 3 (2005) 135-141
23. Zielinski H., and Kozlowska H. Antioxidant activity and total phenolics in selected cereal grains, their different morphological fractions. J. Agri. Food Chem. 48 (2000) 2008-2016