The cAMP-dependent protein kinase inhibitor H-89 attenuates the bioluminescence signal produced by Renilla luciferase

PLoS ONE

Volumn 4, Issue 5, 2009, Pages

  Herbst, Katie J ^a   Allen, Michael D ^a   Zhang, Jin ^a  

^a JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

FIREFLY LUCIFERASE; KT 5720; N [2 (4 BROMOCINNAMYLAMINO)ETHYL] 5 ISOQUINOLINESULFONAMIDE; RENILLA LUCIFERIN 2 MONOOXYGENASE; CYCLIC AMP DEPENDENT PROTEIN KINASE; ISOQUINOLINE DERIVATIVE; MUTANT PROTEIN; PROTEIN KINASE INHIBITOR; SULFONAMIDE;

ARTICLE; BIOLUMINESCENCE; COELENTERATE; CONTROLLED STUDY; ENZYMATIC ASSAY; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME INHIBITION; ENZYME SUBSTRATE; HUMAN; HUMAN CELL; IC 50; IN VITRO STUDY; PROTEIN FUNCTION; PROTEIN PHOSPHORYLATION; RENILLA; SIGNAL TRANSDUCTION; CELL LINE; DRUG ANTAGONISM; DRUG EFFECT; LUMINESCENCE; METABOLISM;

RENILLA LUCIFERASE;

CELL LINE; CYCLIC AMP-DEPENDENT PROTEIN KINASES; HUMANS; ISOQUINOLINES; LUCIFERASES, FIREFLY; LUCIFERASES, RENILLA; LUMINESCENT MEASUREMENTS; MUTANT PROTEINS; PROTEIN KINASE INHIBITORS; SIGNAL TRANSDUCTION; SULFONAMIDES;

EID: 66249095179     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0005642     Document Type: Article

References (30)

1. Cohen P (2002) Protein kinases - the major drug targets of the twenty-first century? Nat Rev Drug Disc 1: 309-315.
2. Zhang J, Allen MD (2007) FRET-based biosensors for protein kinases: illuminating the kinome. Mol BioSyst 3: 759-765.
3. Fan F, Wood KV (2007) Bioluminescence Assays for High-Throughput Screening. ASSAY and Drug Dev Technol 5: 127-136.
4. Inglese J, Johnson RL, Simeonov A, Xia M, Zheng W, et al. (2007) High-throughput screening assays for the identification of chemical probes. Nat Chem Biol 3: 466-478.
5. Brovko LY, Griffiths MW (2007) Illuminating cellular physiology: Recent development. Sci Prog 90: 129-160.
6. Stefan E, Aquin S, Berger N, Landry CR, Nyfeler B, et al. (2007) Quantification of dynamic protein complexes using Renilla luciferase fragment complementation applied to protein kinase A activities in vivo. Proc Natl Acad Sci U S A 104: 16916-16921.
7. Fan F, Binkowski BF, Butler BL, Stecha PF, Lewis MK, et al. (2008) Novel genetically encoded biosensors using firefly luciferase. ACS Chem Biol 3: 346-351.
8. Zhang L, Lee KC, Bhojani MS, Khan AP, Shilman A, et al. (2007) Molecular imaging of Akt kinase activity. Nat Med 13: 1114-1119.
9. Taylor SS, Kim C, Cheng CY, Brown HJS, Wu J, et al. (2008) Signaling through cAMP and cAMP-dependent protein kinase: Diverse strategies for drug design. Biochem et Biophysica Acta 1784: 16-26.
10. Rosenberg D, Groussin L, Jullian E, Perlemone K, Bertagna X, et al. (2006) Role of PKA-regulated transcription factor CREB in development and tumorigenesis of endocrine tissues. Ann N Y Acad Sci 968: 65-74.
11. Matyakhina L, Lenherr SM, Stratakis CA (2006) Protein Kinase A and chromosomal stability. Ann N Y Acad Sci 968: 148-157.
12. Costanzo V, Avvedimento EV, Gottesman ME, Gautier J, Grieco D (1999) Protein Kinase A is required for chromosomal DNA replication. Curr Biol 9: 903-906.
13. Andorfer CA, Davies P (2000) PKA phosphorylates on Tau: Developmental studies in the mouse. Dev Neurosci 22: 303-309.
14. Miller WR (2006) Regulatory subunits of PKA and breast cancer. Ann N Y Acad Sci 968: 37-48.
15. Ruehr ML, Russell MA, Bond M (2004) A-kinase anchoring protein targeting of protein kinase A in the heart. J Mol Cell Cardiol 37: 653-65.
16. Nesher R, Anteby E, Yedivizky M, Warwar N, Kaiser N, et al. (2002) Beta-cell protein kinases and the dynamics of insulin response to glucose. Diabetes 51: S68-S73.
17. Chijiwa T, Mishima A, Hagiwara M, Sano M, Hayashi K, et al. (1990) Inhibition of forskolin-induced neurite outgrowth and protein phosphorylation by a synthesized selective inhibitor of cyclic AMP-dependent protein kinase, N-[2-(p-bromocinnamylamino)ethyl]-5-isoquinolinesulfonamide (H-89), of PC12D pheochromoctoma cells. J Biol Chem 265: 5267-5272.
18. Loening AM, Fenn TD, Wu AM, Gambhir SS (2006) Consensus guided mutagenesis of Renilla luciferase yields enhanced stability and light output. Pro Eng Des & Sel 19: 391-400.
19. Dalton GD, Dewey WL (2006) Protein kinase inhibitor peptide (PKI): A family of endogenous neuropeptides that modulate neuronal cAMP-dependent protein kinase function. Neuropeptides 40: 23-34.
20. Allen MD, Zhang J (2006) Subcellular dynamics of protein kinase A activity visualized by FRET-based reporters. Biochem Biophsy Res Comm 348: 716-721.
21. Zhang J, Hupfeld CJ, Taylor SS, Olefsky JM, Tsien RY (2005) Insulin disrupts b-adrenergic signaling to protein kinase A in adipocytes. Nature 437: 569-573.
22. Kase H, Iwahashi K, Nakanishi S, Matsuda Y, Yamada K, et al. (1987) K-252 Compounds, novel and potent inhibitors of protein kinase c and cyclic nucleotide-dependent protein kinases. Biochm Biophys Res Comm 142: 436-440.
23. Okada Y, Sato-Yoshitake R, Hirokawa N (1995) The activation of protein kinase A pathway selectively inhibits anterograde axonal transport of vesicles but not mitochondrial transport or retrograde transport in vivo. J Neurosci 15: 3053-3064.
24. Bakhtiarova A, Taslimi P, Elliman SJ, Kosinski PA, Hubbard B, et al. (2006) Resveratrol inhibits firefly luciferase. Biochem Biophys Res Comm 351: 481-484.
25. Deroo BJ, Archer TK (2002) Proteasome inhibitors reduce luciferase and β-galactosidase activity in tissue culture cells. J Biol Chem 277: 20120-20123.
26. Rocha S, Campbell KJ, Roche KC, Perkins ND (2003) The p53 inhibitor Pifithrin-α inhibits firefly luciferase activity in vivo and in vitro. BMC Mol Biol 4: 1-8.
27. Auld DS, Southall NL, Jadhav A, Johnson RL, Diller DJ, et al. (2008) Characterization of chemical libraries for luciferase inhibitory activity. J Med Chem 51: 2372-2386.
28. Auld DS, Thorne N, Maguire WF, Inglese J (2009) Mechanism of PTC124 activity in cell-based luciferase assays of nonsense codon suppression. Proc Natl Acad Sci U S A 106: 3585-3590.
29. Woo J, Howell MH, von Arnim AG (2008) Structure function studies on the active site of the coelenterazine-depedent luciferase from Renilla. Pro Sci 17: 725-735.
30. Nardini M, Dijkstra BW (1999) α/β hydrolase fold enzymes: the family keeps growing. Cur Opin Struc Biol 9: 732-737.