Subcellular dynamics of protein kinase A activity visualized by FRET-based reporters

Biochemical and Biophysical Research Communications

Volumn 348, Issue 2, 2006, Pages 716-721

  Allen, Michael D ^a   Zhang, Jin ^a  

^a JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Circular permutation; Compartmentalization; FRET; Kinase; Live cell imaging; Mitochondria; PKA

Indexed keywords

A KINASE ACTIVITY REPORTER 3 PROTEIN; CYCLIC AMP DEPENDENT PROTEIN KINASE; FLUORESCENT DYE; PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; CELL COMPARTMENTALIZATION; CELL MEMBRANE; CELL NUCLEUS; CELL STRAIN HEK293; CELLULAR DISTRIBUTION; CYTOPLASM; ENZYME ACTIVITY; FLUORESCENCE RESONANCE ENERGY TRANSFER; HUMAN; HUMAN CELL; MITOCHONDRION; MOLECULAR DYNAMICS; PRIORITY JOURNAL; REGULATORY MECHANISM;

AMINO ACID SEQUENCE; BACTERIAL PROTEINS; CELL COMPARTMENTATION; CELL MEMBRANE; CELL NUCLEUS; CELLS, CULTURED; CYCLIC AMP-DEPENDENT PROTEIN KINASES; CYTOPLASM; FLUORESCENCE RESONANCE ENERGY TRANSFER; GREEN FLUORESCENT PROTEINS; HUMANS; LUMINESCENT PROTEINS; MITOCHONDRIA; RECOMBINANT PROTEINS;

EID: 33746907024     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2006.07.136     Document Type: Article

References (23)

1. Taylor S.S., Kim C., Vigil D., Haste N.M., Yang J., Wu J., and Anand G.S. Dynamics of signaling by PKA. Biochim. Biophys. Acta 1754 (2005) 25-37
2. Wong W., and Scott J.D. AKAP signalling complexes: focal points in space and time. Nat. Rev. Mol. Cell Biol. 5 (2004) 959-970
3. Tasken K., and Aandahl E.M. Localized effects of cAMP mediated by distinct routes of protein kinase A. Physiol. Rev. 84 (2004) 137-167
4. Baillie G.S., Scott J.D., and Houslay M.D. Compartmentalisation of phosphodiesterases and protein kinase A: opposites attract. FEBS Lett. 579 (2005) 3264-3270
5. Miyawaki A. Visualization of the spatial and temporal dynamics of intracellular signaling. Dev. Cell 4 (2003) 295-305
6. Zhang J., Campbell R.E., Ting A.Y., and Tsien R.Y. Creating new fluorescent probes for cell biology. Nat. Rev. Mol. Cell Biol. 3 (2002) 906-918
7. Zhang J., Ma Y., Taylor S.S., and Tsien R.Y. Genetically encoded reporters of protein kinase A activity reveal impact of substrate tethering. Proc. Natl. Acad. Sci. USA 98 (2001) 14997-15002
8. Zhang J., Hupfeld C.J., Taylor S.S., Olefsky J.M., and Tsien R.Y. Insulin disrupts beta-adrenergic signalling to protein kinase A in adipocytes. Nature 437 (2005) 569-573
9. Q. Ni, D.V. Titov, J. Zhang, Analyzing protein kinase dynamics in living cells with FRET reporters, Methods, 2006, in press.
10. Dodge-Kafka K.L., Soughayer J., Pare G.C., Carlisle Michel J.J., Langeberg L.K., Kapiloff M.S., and Scott J.D. The protein kinase A anchoring protein mAKAP coordinates two integrated cAMP effector pathways. Nature 437 (2005) 574-578
11. Nagai T., Ibata K., Park E.S., Kubota M., Mikoshiba K., and Miyawaki A. A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat. Biotechnol. 20 (2002) 87-90
12. Nagai T., Yamada S., Tominaga T., Ichikawa M., and Miyawaki A. Expanded dynamic range of fluorescent indicators for Ca(2+) by circularly permuted yellow fluorescent proteins. Proc. Natl. Acad. Sci. USA 101 (2004) 10554-10559
13. Baird G.S., Zacharias D.A., and Tsien R.Y. Circular permutation and receptor insertion within green fluorescent proteins. Proc. Natl. Acad. Sci. USA 96 (1999) 11241-11246
14. Nguyen A.W., and Daugherty P.S. Evolutionary optimization of fluorescent proteins for intracellular FRET. Nat. Biotechnol. 23 (2005) 355-360
15. Ananthanarayanan B., Ni Q., and Zhang J. Signal propagation from membrane messengers to nuclear effectors revealed by reporters of phosphoinositide dynamics and Akt activity. Proc. Natl. Acad. Sci. USA 102 (2005) 15081-15086
16. Ullman K.S., Powers M.A., and Forbes D.J. Nuclear export receptors: from importin to exportin. Cell 90 (1997) 967-970
17. Ma Y., and Taylor S. A 15-residue bifunctional element in D-AKAP1 is required for both endoplasmic reticulum and mitochondrial targeting. J. Biol. Chem. 277 (2002) 27328-27336
18. Miyawaki A., and Tsien R.Y. Monitoring protein conformations and interactions by fluorescence resonance energy transfer between mutants of green fluorescent protein. Methods Enzymol. 327 (2000) 472-500
19. Tsien R.Y. Building and breeding molecules to spy on cells and tumors. FEBS Lett. 579 (2005) 927-932
20. Meinkoth J.L., Ji Y., Taylor S.S., and Feramisco J.R. Dynamics of the distribution of cyclic AMP-dependent protein kinase in living cells. Proc. Natl. Acad. Sci. USA 87 (1990) 9595-9599
21. Harootunian A.T., Adams S.R., Wen W., Meinkoth J.L., Taylor S.S., and Tsien R.Y. Movement of the free catalytic subunit of cAMP-dependent protein kinase into and out of the nucleus can be explained by diffusion. Mol. Biol. Cell 4 (1993) 993-1002
22. DiPilato L.M., Cheng X., and Zhang J. Fluorescent indicators of cAMP and Epac activation reveal differential dynamics of cAMP signaling within discrete subcellular compartments. Proc. Natl. Acad. Sci. USA 101 (2004) 16513-16518
23. Allen M.D., DiPilato L.M., Rahdar M., Ren Y.R., Chong C., Liu J.O., and Zhang J. Reading dynamic kinase activity in living cells for high-throughput screening. ACS Chem. Biol. 1 (2006) 371-376