Divergent flow isoelectric focusing: Fast and efficient method for protein sample preparation for mass spectrometry

Analytical and Bioanalytical Chemistry

Volumn 393, Issue 6-7, 2009, Pages 1769-1778

  Mazanec, Karel ^a   Bobalova, Janette ^a   Šlais, Karel ^a  

^a INSTITUTE OF ANALYTICAL CHEMISTRY   (Czech Republic)

Author keywords

Glycation; Isoelectric focusing; MALDI MS; Post translational modifications; Protein

Indexed keywords

AMPHOTERIC COMPOUNDS; BIOLOGICAL SAMPLES; CHANNEL SIDE; CRUDE PROTEINS; CUT OUT; DC VOLTAGE; DIVERGENT FLOW; EFFICIENT METHOD; GLYCATION; ISO-ELECTRIC POINTS; ISOELECTRIC FOCUSING; LAY-OUT; MALDI-MS; MASS SPECTRA; NONWOVEN WEBS; PH GRADIENTS; POST-TRANSLATIONAL MODIFICATIONS; PROTEIN MIXTURES; PROTEIN SAMPLES; PROTEOMIC STUDIES; SAMPLE PREPARATION; SEPARATION AND PURIFICATION; SEPARATION CHANNEL; SEPARATION TECHNIQUES;

BEVERAGES; DESALINATION; ELECTRIC POTENTIAL; FLUORESCENCE; FOCUSING; MASS SPECTROMETERS; MASS SPECTROMETRY; PROTEINS; PURIFICATION; SEPARATION;

ELECTROPHORESIS;

HORDEUM;

PROTEIN;

ANALYSIS; ARTICLE; ELECTRODE; EQUIPMENT DESIGN; INSTRUMENTATION; ISOELECTRIC FOCUSING; MASS SPECTROMETRY; METHODOLOGY; MOLECULAR WEIGHT; PH; PROTEOMICS; REPRODUCIBILITY; SENSITIVITY AND SPECIFICITY; TIME;

ANALYTIC SAMPLE PREPARATION METHODS; ELECTRODES; EQUIPMENT DESIGN; HYDROGEN-ION CONCENTRATION; ISOELECTRIC FOCUSING; MOLECULAR WEIGHT; PROTEINS; PROTEOMICS; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; TIME FACTORS;

EID: 66149118996     PISSN: 16182642     EISSN: 16182650     Source Type: Journal    
DOI: 10.1007/s00216-009-2600-8     Document Type: Article

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