Investigation of protein composition of barley by gel electrophoresis and MALDI mass spectrometry with regard to the malting and brewing process

Journal of the Institute of Brewing

Volumn 114, Issue 1, 2008, Pages 22-26

  Bobalova, Janette ^a   Salplachta, Jiri ^a   Chmelik, Josef ^a  

^a INSTITUTE OF ANALYTICAL CHEMISTRY   (Czech Republic)

Author keywords

Barley; Barley proteins; MALDI; Mass spectrometry; Matrix; Protein pattern

Indexed keywords

HORDEUM;

EID: 43249107424     PISSN: 00469750     EISSN: None     Source Type: Journal    
DOI: 10.1002/j.2050-0416.2008.tb00301.x     Document Type: Article

References (11)

1. 1 Bobalova, J. and Chmelik J., Proteomic identification of technologically modified proteins in malt by combination of protein fractionation using convective interaction media and matrixassisted laser desorption/ionization time‐of‐flight mass spectrometry. J. Chromatogr. A, 2007, 1163, 80–85.
2. 2 Čížková, H., Dostálek, P., Fiala, J. and Kolouchová, I., Importance of proteins from the viewpoint of stability of the beer foam. Chemicke listy, 2006, 100, 478–485.
3. 3 Finnie, C., Maeda, K., Ostergaard, O., Bak‐Jensen, K.S., Larsen, J. and Svensson, B., Aspects of the barley seed proteome during development and germination. Biochemical Society Transactions, 2004, 32, 517–519.
4. 4 Hao, J., Li, Q., Dong, J.J., Yu, J.J., Gu, G.X., Fan, W. and Chen, J., Identification of the major proteins in beer foam by mass spectrometry following sodium dodecyl sulfate‐polyacrylamide gel electrophoresis. Journal of the American Society of Brewing Chemists, 2006, 64, 166–174.
5. 5 Jégou, S., Douliez, J‐P., Mollé, D., Boivin, P. and Marion, D., Evidence of the glycation and denaturation of LTP1 during the malting and brewing process. J. Agric. Food Chem., 2001, 49, 4942–4949.
6. 6 Jégou, S., Douliez, J‐P., Mollé, D., Boivin, P. and Marion, D., Purification and structural characterization of LTP1 polypeptides from beer. J. Agric. Food Chem., 2000, 48, 5023–5029.
7. 7 Kussmann, M., Nordhoff, E., Rahbek‐Nielsen, H., Haebel, S., Rossel‐Larsen, M., Jakobsen, L., Gobom, J., Mirgorodskaya, E., Kroll‐Kristensen, A., Palm, L. and Roepstorff, P., Matrix‐assisted laser desorption/ionization mass spectrometry sample preparation techniques designed for various peptide and protein analytes. J. Mass Spectrom., 1997, 32, 593–601.
8. 8 Leisegang, R. and Stahl, U., Degradation of a foam‐promoting barley protein by a proteinase from brewing yeast. Journal of the Institute of Brewing, 2005, 111, 112–117.
9. 9 Perrocheau, L., Rogniaux, H., Boivin, P. and Marion, D., Probing heat‐stable water‐soluble proteins from barley to malt and beer. Proteomics, 2005, 5, 2849–2858.
10. 10 Prokeš, J., Vaculová, K., Milotová, J., Selected malting parameters of new accessions from the collection of spring barley genetic resources? Kvasny Prum. 2007, 53 (6), 162–167.
11. 11 Shevchenko, A., Wilm, M., Vorm, O. and Mann, M., Mass spectrometric sequencing of proteins from silver stained polyacrylamide gels. Anal. Chem., 1996, 68, 850–858.