Cloning and sequencing an inverted repeat

Cold Spring Harbor Protocols

Volumn 4, Issue 1, 2009, Pages

  Svoboda, Petr ^a  

^a INSTITUTE OF MOLECULAR GENETICS   (Czech Republic)

Author keywords

[No Author keywords available]

Indexed keywords

COMPLEMENTARY DNA; SPACER DNA;

ARTICLE; DNA STRUCTURE; GENE EXPRESSION; GENE SEQUENCE; GENOME SIZE; INVERTED REPEAT; MOLECULAR CLONING; MOLECULAR GENETICS; NONHUMAN; POLYMERASE CHAIN REACTION; RESTRICTION SITE; RNA INTERFERENCE; SEQUENCE ANALYSIS; TRANSGENE;

EID: 65649153004     PISSN: None     EISSN: 15596095     Source Type: Journal    
DOI: 10.1101/pdb.ip64     Document Type: Article

References (7)

1. Kalidas, S. and Smith D, P. 2002. Novel genomic cDNA hybrids produce effective RNA interference in adult Drosophila. Neuron 33: 177-184.
2. Smith, N.A., Singh, S.P., Wang, M.B., Stoutjesdijk, P.A., Green, A.G., and Waterhouse, P.M. 2000. Total silencing by intron-spliced hairpin RNAs. Nature 407: 319-320.
3. Svoboda, P. 2009a. Cloning a transgene for transgenic RNAi in mouse oocytes. Cold Spring Harb. Protoc. (this issue). doi: 10.1101/ pdb.prot5134.
4. Svoboda, P. 2009b. Preparation of dsRNA for microinjection experiments in mouse. Cold Spring Harb. Protoc. (this issue). doi: 10.1101/ pdb.prot5131.
5. Svoboda, P. 2009c. Choosing the sequence of dsRNA for RNAi in mouse. Cold Spring Harb. Protoc. (this issue). doi: 10.1101/pdb.ip63.
6. Svoboda, P., Stein, P., and Schultz, R.M. 2001. RNAi in mouse oocytes and preimplantation embryos: Effectiveness of hairpin dsRNA. Biochem. Biophys. Res. Commun. 287: 1099-1104.
7. Wesley, S.V., Helliwell, C.A., Smith, N.A., Wang, M.B., Rouse, D.T., Liu, Q., Gooding, P.S., Singh, S.P., Abbott, D., Stoutjesdijk, P.A., et al. 2001. Construct design for efficient, effective and high-throughput gene silencing in plants. Plant J. 27: 581-590.