Cloning a transgene for transgenic RNAi in mouse oocytes

Cold Spring Harbor Protocols

Volumn 4, Issue 1, 2009, Pages

  Svoboda, Petr ^a  

^a INSTITUTE OF MOLECULAR GENETICS   (Czech Republic)

Author keywords

[No Author keywords available]

Indexed keywords

COMPLEMENTARY DNA; RNA;

ANIMAL CELL; ARTICLE; CONTROLLED STUDY; CULTURE MEDIUM; DNA EXTRACTION; ELECTROPORATION; GENE CASSETTE; GENE CONSTRUCT; GENE INSERTION; INOCULATION; INVERTED REPEAT; MOLECULAR CLONING; MOUSE; NONHUMAN; OOCYTE; PROMOTER REGION; PROTEIN DEPHOSPHORYLATION; RNA INTERFERENCE; RNA PURIFICATION; TRANSGENE; TRANSGENIC MOUSE;

EID: 65649137752     PISSN: None     EISSN: 15596095     Source Type: Journal    
DOI: 10.1101/pdb.prot5134     Document Type: Article

References (7)

1. Barbas III, C.F., Burton, D.R., Scott, J.K., and Silverman, G.J. 2007. Quantitation of DNA and RNA. Cold Spring Harb. Protoc. doi: 10.1101/pdb.ip47.
2. Sambrook, J. and Russell, D.W. 2006a. Purification of nucleic acids by extraction with phenol:chloroform. Cold Spring Harb. Protoc. doi: 10.1101/pdb.prot4455.
3. Sambrook, J. and Russell, D.W. 2006b. Standard ethanol precipitation of DNA in microcentrifuge tubes. Cold Spring Harb. Protoc. doi: 10.1101/pdb.prot4456.
4. Sambrook, J. and Russell, D.W. 2006c. Preparation of plasmid DNA by alkaline lysis with SDS: Minipreparation. Cold Spring Harb. Protoc. doi: 10.1101/pdb.prot4084.
5. Stein, P. 2009. Microinjection of plasmids into meiotically incompetent oocytes. Cold Spring Harb. Protoc. (this issue). doi: 10.1101/ pdb.prot5135.
6. Svoboda, P. 2009. Cloning and sequencing an inverted repeat. Cold Spring Harb. Protoc. (this issue). doi: 10.1101/pdb.ip64.
7. Svoboda, P. and Stein, P. 2009. RNAi experiments in mouse oocytes and early embryos. Cold Spring Harb. Protoc. (this issue). doi: 10.1101/pdb.top56.