Preparation of dsRNA for microinjection experiments in mouse

Cold Spring Harbor Protocols

Volumn 4, Issue 1, 2009, Pages

  Svoboda, Petr ^a  

^a INSTITUTE OF MOLECULAR GENETICS   (Czech Republic)

Author keywords

[No Author keywords available]

Indexed keywords

DOUBLE STRANDED DNA; RNA POLYMERASE;

AGAR GEL ELECTROPHORESIS; ANIMAL CELL; ARTICLE; CENTRIFUGATION; CONTROLLED STUDY; CRYOPRESERVATION; EMBRYO; EMBRYO CELL; EXPERIMENTAL MOUSE; INCUBATION TIME; MICROINJECTION; MOUSE; NONHUMAN; PROMOTER REGION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA DEGRADATION; RNA PURIFICATION; RNA TRANSCRIPTION; TRANSGENIC MOUSE;

EID: 65649147571     PISSN: None     EISSN: 15596095     Source Type: Journal    
DOI: 10.1101/pdb.prot5131     Document Type: Article

References (9)

1. Sambrook, J. and Russell, D.W. 2006a. Purification of nucleic acids by extraction with phenol:chloroform. Cold Spring Harb. Protoc. doi: 10.1101/pdb.prot4455.
2. Sambrook, J. and Russell, D.W. 2006b. Standard ethanol precipitation of DNA in microcentrifuge tubes. Cold Spring Harb. Protoc. doi: 10.1101/pdb.prot4456.
3. Stein, P. 2009a. Microinjection of dsRNA into fully-grown mouse oocytes. Cold Spring Harb. Protoc. (this issue). doi: 10.1101/pdb.prot5132.
4. Stein, P. 2009b. Microinjection of dsRNA into mouse one-cell embryos. Cold Spring Harb. Protoc. (this issue). doi: 10.1101/pdb.prot5133.
5. Stein, P. and Svoboda, P. 2006. Preparation of dsRNA molecules for RNAi in mouse oocytes and early embryos. Cold Spring Harb. Protoc. doi: 10.1101/pdb.prot4508.
6. Svoboda, P. 2009a. Choosing the sequence of dsRNA for RNAi in mouse. Cold Spring Harb. Protoc. (this issue). doi: 10.1101/pdb.ip63.
7. Svoboda, P. 2009b. Cloning a transgene for transgenic RNAi in mouse oocytes. Cold Spring Harb. Protoc. (this issue). doi: 10.1101/ pdb.prot5134.
8. Svoboda, P. and Stein, P. 2009. RNAi experiments in mouse oocytes and early embryos. Cold Spring Harb. Protoc. (this issue). doi: 10.1101/pdb.top56.
9. Svoboda, P., Stein, P., Hayashi, H., and Schultz, R.M. 2000. Selective reduction of dormant maternal mRNAs in mouse oocytes by RNA interference. Development 127: 4147-4156.