Genetic dissection of toll-like receptor signaling using ENU mutagenesis

Methods in Molecular Biology

Volumn 517, Issue , 2009, Pages 239-251

  Hoebe, Kasper ^a  

^a SCRIPPS RESEARCH INSTITUTE   (United States)

Author keywords

ENU; Genotype; Mapping; Mouse; Mutagenesis; Phenotype; TLR

Indexed keywords

MUS;

ETHYLNITROSOUREA; TOLL LIKE RECEPTOR;

ANIMAL; ARTICLE; CHROMOSOME MAP; DRUG EFFECT; FEMALE; GENETICS; HOMOZYGOTE; MACROPHAGE; MALE; METABOLISM; MOUSE; MUTAGENESIS; PEDIGREE; PHENOTYPE; SIGNAL TRANSDUCTION;

ANIMALS; CHROMOSOME MAPPING; ETHYLNITROSOUREA; FEMALE; HOMOZYGOTE; MACROPHAGES; MALE; MICE; MUTAGENESIS; PEDIGREE; PHENOTYPE; SIGNAL TRANSDUCTION; TOLL-LIKE RECEPTORS;

EID: 65649108946     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-59745-541-1_15     Document Type: Article

References (16)

1. Brunkow, M. E., Jeffery, E. W., Hjerrild, K. A., Paeper, B., Clark, L. B., Yasayko, S. A., Wilkinson, J. E., Galas, D., Ziegler, S. F., and Ramsdell, F. (2001) Disruption of a new fork-head/winged-helix protein, scurfin, results in the fatal lymphoproliferative disorder of the scurfy mouse. Nat. Genet. 27, 68-73. (Pubitemid 32044521)
2. Lee, S. H., Webb, J. R., and Vidal, S. M. (2002) Innate immunity to cytomegalovirus: the Cmv1 locus and its role in natural killer cell function. Microbes. Infect. 4, 1491-1503. (Pubitemid 36050818)
3. Scalzo, A. A., Wheat, R., Dubbelde, C., Stone, L., Clark, P., Du, Y., Dong, N., Stoll, J., Yokoyama, W. M., and Brown, M. G. (2003) Molecular genetic characterization of the distal NKC recombination hotspot and putative murine CMV resistance control locus. Immu-nogenetics 55, 370-378. (Pubitemid 37255488)
4. Webb, J. R., Lee, S. H., and Vidal, S. M. (2002) Genetic control of innate immune responses against cytomegalovirus: MCMV meets its match. Genes Immun. 3, 250-262. (Pubitemid 38967550)
5. Poltorak, A., He, X., Smirnova, I., Liu, M. Y., Van Huffel, C., Du, X., Birdwell, D., Ale-jos, E., Silva, M., Galanos, C., Freudenberg, M., Ricciardi-Castagnoli, P., Layton, B., and Beutler, B. (1998) Defective LPS signaling in c3h/hej and c57bl/10sccr mice: mutations in tlr4 gene. Science 282, 2085-2088. (Pubitemid 29001265)
6. Hoebe, K., Georgel, P., Rutschmann, S., Du, X., Mudd, S., Crozat, K., Sovath, S., Shamel, L., Hartung, T., Zahringer, U., and Beutler, B. (2005) CD36 is a sensor of diacylglycer-ides. Nature 433, 523-527.
7. Balling, R. (2001) ENU mutagenesis: analyzing gene function in mice. Annu. Rev. Genom-ics Hum. Genet. 2, 463-492. (Pubitemid 35265015)
8. Favor, J., Neuhauser-Klaus, A., and Ehling, U. H. (1998) The effect of dose fractionation on the frequency of ethylnitrosourea-induced dominant cataract and recessive specific locus mutations in germ cells of the mouse. Mutat. Res. 198, 269-275.
9. Favor, J., Neuhauser-Klaus, A., Ehling, U. H., Wulff, A., and van Zeeland, A. A. (1997) The effect of the interval between dose applications on the observed specific-locus mutation rate in the mouse following fractionated treatments of spermatogonia with ethylnitrosou-rea. Mutat. Res. 374, 193-199. (Pubitemid 27122930)
10. Rinchik, E. M., Carpenter, D. A., and Selby, P. B. (1990) A strategy for fine-structure functional analysis of a 6-to 11-centimorgan region of mouse chromosome 7 by high-efficiency mutagenesis. Proc. Natl. Acad. Sci. U. S. A. 87, 896-900.
11. Rinchik, E. M. and Carpenter, D. A. (1999) N-ethyl-N-nitrosourea mutagenesis of a 6-to 11-cM subregion of the Fah-Hbb interval of mouse chromosome 7: Completed testing of 4557 gametes and deletion mapping and complementation analysis of 31 mutations. Genetics 152, 373-383.
12. Russell, W. L., Hunsicker, P. R., Carpenter, D. A., Cornett, C. V., and Guinn, G. M. (1982) Effect of dose fractionation on the ethylnitro-sourea induction of specific-locus mutations in mouse spermatogonia. Proc. Natl. Acad. Sci. U. S. A. 79, 3592-3593. (Pubitemid 12014515)
13. Russell, W. L., Hunsicker, P. R., Raymer, G. D., Steele, M. H., Stelzner, K. F., and Thompson, H. M. (1982) Dose-response curve for ethylnitrosourea- induced specific-locus mutations in mouse spermatogonia. Proc. Natl. Acad. Sci. U. S. A. 79, 3589-3591. (Pubitemid 12014514)
14. Justice, M. J., Noveroske, J. K., Weber, J. S., Zheng, B., and Bradley, A. (1999) Mouse ENU mutagenesis. Hum. Mol. Genet. 8, 1955-1963. (Pubitemid 29458674)
15. Concepcion, D., Seburn, K. L., Wen, G., Frankel, W. N., and Hamilton, B. A. (2004) Mutation rate and predicted phenotypic target sizes in ethylnitrosourea-treated mice. Genetics 168, 953-959. (Pubitemid 39491470)
16. Hoebe, K., Du, X., Goode, J., Mann, N., and Beutler, B. (2003) Lps2: a new locus required for responses to lipopolysaccha-ride, revealed by germline mutagenesis and phenotypic screening. J. Endotoxin. Res. 9, 250-255. (Pubitemid 36874536)