Immobilized metal affinity chromatography in open-loop simulated moving bed technology: Purification of a heat stable histidine tagged β-glucosidase

Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

Volumn 877, Issue 16-17, 2009, Pages 1651-1656

  Sahoo, Deepti ^a   Andersson, Jonatan ^a   Mattiasson, Bo ^a  

^a LUND UNIVERSITY   (Sweden)

Author keywords

His tag; IMAC; SMB

Indexed keywords

E. COLI; GLUCOSIDASE; HIS TAG; IMAC; IMMOBILIZED METAL AFFINITY CHROMATOGRAPHIES; IMMOBILIZED METALS; ONE STEPS; OPEN LOOPS; PURIFICATION FACTORS; SIMULATED MOVING BED TECHNOLOGIES; SIMULATED MOVING BEDS; SINGLE BANDS; SMB;

AFFINITY CHROMATOGRAPHY; CHROMATOGRAPHIC ANALYSIS; COLUMN CHROMATOGRAPHY; ESCHERICHIA COLI; METAL RECOVERY; PURIFICATION;

METAL REFINING;

BETA GLUCOSIDASE; BUFFER; POLYHISTIDINE TAG;

AFFINITY CHROMATOGRAPHY; ARTICLE; ELUTION; ESCHERICHIA COLI; HEAT; NONHUMAN; PRIORITY JOURNAL; PRODUCTIVITY; PURIFICATION; TECHNOLOGY;

BETA-GLUCOSIDASE; CHROMATOGRAPHY, AFFINITY; ESCHERICHIA COLI; HISTIDINE; HOT TEMPERATURE; METALS; OLIGOPEPTIDES; PROTEIN STABILITY; RECOMBINANT FUSION PROTEINS; THERMOTOGA NEAPOLITANA;

EID: 65549115827     PISSN: 15700232     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jchromb.2009.04.005     Document Type: Article

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