Probing active-site residues of pyranose 2-oxidase from Trametes multicolor by semi-rational protein design

Biotechnology Journal

Volumn 4, Issue 4, 2009, Pages 535-543

  Salaheddin, Clara ^a,c   Spadiut, Oliver ^a   Ludwig, Roland ^a,c   Tan, Tien Chye ^b   Divne, Christina ^b   Haltrich, Dietmar ^a,d   Peterbauer, Clemens ^a,d  

^a UNIVERSITY OF NATURAL RESOURCES AND LIFE SCIENCES   (Austria)

^b ALBANOVA UNIVERSITY CENTER   (Sweden)

^c RESEARCH CENTRE APPLIED BIOCATALYSIS   (Austria)

^d Department Biotechnology   (Austria)

Author keywords

Protein design; Pyranose 2 oxidase; Saturation mutagenesis

Indexed keywords

96-WELL MICROTITER PLATES; ACTIVE SITES; CATALYTIC EFFICIENCIES; D GALACTOSE; D GLUCOSE; D-TAGATOSE; ELECTRON ACCEPTORS; ENZYMATIC OXIDATIONS; KETO DERIVATIVES; KINETIC INVESTIGATIONS; KINETIC PROPERTIES; MICHAELIS-MENTEN KINETICS; PROTEIN DESIGN; PYRANOSE 2-OXIDASE; RATIONAL DESIGNS; SATURATION MUTAGENESIS; SUBSTRATE BINDINGS; WILD TYPES;

DESIGN; GLUCOSE; MUTAGENESIS; OXIDATION; OXYGEN; SUGAR (SUCROSE);

SUBSTRATES;

GALACTOSE; GLUCOSE; OXIDOREDUCTASE; PYRANOSE 2 OXIDASE; UNCLASSIFIED DRUG;

ARTICLE; BIOTECHNOLOGICAL PRODUCTION; BIOTRANSFORMATION; CATALYSIS; CONTROLLED STUDY; ENZYME ACTIVE SITE; ENZYME BINDING; ENZYME KINETICS; ENZYME SUBSTRATE; GENE LIBRARY; MUTAGENESIS; MUTANT; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN STRUCTURE; TRAMETES; TRAMETES MULTICOLOR; WILD TYPE;

AMINO ACID SEQUENCE; BINDING SITES; BIOCATALYSIS; BIOREACTORS; BUFFERS; CARBOHYDRATE DEHYDROGENASES; ESCHERICHIA COLI; GALACTOSE; GENE EXPRESSION; GENETIC VECTORS; HYDROGEN BONDING; HYDROGEN-ION CONCENTRATION; KINETICS; MODELS, MOLECULAR; MODELS, THEORETICAL; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; MUTATION; OXIDATION-REDUCTION; PHOSPHATES; PROTEIN BINDING; PROTEIN ENGINEERING; PROTEIN STRUCTURE, SECONDARY; RECOMBINANT PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID; STRUCTURE-ACTIVITY RELATIONSHIP; SUBSTRATE SPECIFICITY; TEMPERATURE; TRAMETES;

TRAMETES OCHRACEA;

EID: 65549111724     PISSN: 18606768     EISSN: 18607314     Source Type: Journal    
DOI: 10.1002/biot.200800265     Document Type: Article

References (28)

1. Volc, J., Denisova, N. P., Nerud, F., Musílek, V., Glucose-2-oxidase activity in mycelial cultures of basidiomycetes. Folia Microbiol. 1985, 30, 141-147.
2. Leitner, C., Haltrich, D., Nidetzky, B., Prillinger, H., Kulbe, K. D., Production of a novel pyranose 2-oxidase by basidiomycete Trametes multicolor. Appl. Biochem. Biotechnol. 1998, 70-72, 237-248.
3. Ander, P., Marzullo, L., Sugar oxidoreductases and veratryl alcohol oxidase as related to lignin degradation. J. Biotechnol. 1997, 53, 115-131.
4. Volc, J., Kubátová, E., Daniel, G., Prikrylová, V., Only C-2 specific glucose oxidase activity is expressed in ligninolytic cultures of the white rot fungus Phanerochaete chrysosporium. Arch. Microbiol. 1996, 165, 421-424.
5. Ruelius, H.W., Kerwin, R. M., Janssen, F.W., Carbohydrate oxidase, a novel enzyme from Polyporus obtusus. I. Isolation and purification. Biochim. Biophys. Acta 1968, 167, 493-500.
6. Leitner, C., Volc, J., Haltrich, D., Purification and characterization of pyranose oxidase from the white rot fungus Trametes multicolor. Appl. Environ. Microbiol. 2001, 67, 3636-3644.
7. Albrecht, M., Lengauer, T., Pyranose oxidase identified as a member of the GMC oxidoreductase family. Bioinformatics 2003, 19, 1216-1220.
8. Hallberg, B. M., Leitner, C., Haltrich, D., Divne, C., Crystallization and preliminary X-ray diffraction analysis of pyranose 2-oxidase from the white-rot fungus Trametes multicolor. Acta Crystallogr.D. Biol. Crystallogr. 2004, 60, 197-199.
9. Halada, P., Leitner, C., Sedmera, P., Haltrich, D., Volc, J., Identification of the covalent flavin adenine dinucleotide-binding region in pyranose 2-oxidase from Trametes multicolor. Anal. Biochem. 2003, 314, 235-242.
10. Hallberg, B. M., Leitner, C., Haltrich, D., Divne, C., Crystal structure of the 270 kDa homotetrameric lignin-degrading enzyme pyranose 2-oxidase. J. Mol. Biol. 2004, 341, 781-796.
11. Janssen, F.W., Ruelius, H.W., Pyranose oxidase from Polyporus obtusus. Methods Enzymol. 1975, 41, 170-173.
12. Artolozaga, M. J., Kubátová, E., Volc, J., Kalisz, H. M., Pyranose 2-oxidase from Phanerochaete chrysosporium - further biochemical characterisation. Appl. Microbiol. Biotechnol. 1997, 47, 508-514.
13. Massey, V., A simple method for the determination of redox potentials. In: Curti, B., Rochi, S., Zanetti, G. (Eds.), Flavins and Flavoproteins, Walter deGruyter, Berlin 1991, pp. 59-66.
14. Freimund, S., Huwig, A., Giffhorn, F., Köpper, S., Convenient chemo-enzymatic synthesis of D-tagatose. J. Carbohydr. Chem. 1996, 15, 115-120.
15. Giffhorn, F., Fungal pyranose oxidases: occurrence, properties and biotechnical applications in carbohydrate chemistry. Appl. Microbiol. Biotechnol. 2000, 54, 727-740.
16. Kujawa, M., Ebner, H., Leitner, C., Hallberg, B. M. et al., Structural basis for substrate binding and regioselective oxidation of monosaccharides at C3 by pyranose 2-oxidase. J. Biol. Chem. 2006, 281, 35104-35115.
17. Spadiut, O., Leitner, C., Tan, T. C., Ludwig, R. et al., Mutations of Thr169 affect substrate specificity of pyranose 2-oxidase from Trametes multicolor. Biocatal. Biotransfor. 2007, 26, 120-127.
18. Heckman, K. L., Pease, L. R., Gene splicing and mutagenesis by PCR-driven overlap extension. Nat. Protoc. 1997, 2, 924-932.
19. Georgescu, R., High-throughput tetramethylbenzidine (TMB) screen for peroxidases. Methods Mol. Biol. 231, 75-83.
20. Bradford, M. M., A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 1976, 72, 248-254.
21. Fraaije, M.W., Mattevi, A., Flavoenzymes: diverse catalysts with recurrent features. Trends Biochem. Sci. 2000, 25, 126-132.
22. Fraaije, M.W., van Den Heuvel, R. H., van Berkel, W. J., Mattevi, A., Structural analysis of flavinylation in vanillyl-alcohol oxidase. J. Biol. Chem. 2000, 275, 38654-38658.
23. Bayne, S., Fewster, J. A., The osones. Adv. Carbohydr. Chem. 1956, 48, 43-96.
24. Vuorinen, T., Cleavage of D-arabino-hexos-2-ulose and glyoxal with hydrogen peroxide. Carbohydr. Res. 1984, 127, 319-325.
25. Morley, K. L., Kazlauskas, R. J., Improving enzyme properties: When are closer mutations better? Trends Biotechnol. 2005, 23, 231-237.
26. Chica, A. R., Doucet, N., Pelletier, J. N., Semi-rational approaches to engineering enzyme activity: combining the benefits of directed evolution and rational design. Curr. Opin. Biotechnol. 2005, 16, 378-384.
27. Spadiut, O., Radakovits, K., Pisanelli, I., Salaheddin, C. et al., A thermostable triple mutant of pyranose 2-oxidase from Trametes multicolor with improved properties for biotechnological applications. Biotechnol. J. 2009, DOI: 10.1002/biot.200800260.
28. Jones, T.A., Zou, J.-Y., Cowan, S.W., Kjeldgaard, M., Improved methods for building protein models in electron density maps and the location of errors in these models. Acta Crystallogr. Sect. A 1991, 47, 110-119.